细胞计数

And thaw, and cell fusion; Understanding the principles and operation procedures for autoradiograph; Mastering the skills of writing experiment reports and graphic drawing under microscope.

本课程是农业与生物学院本科学生的一门实验课程，本课程主要内容包括：掌握和了解普通光学显微镜、几种特殊显微镜和电镜的一般原理和使用方法；掌握显微镜临时标本的制备，会进行显微测量、细胞计数和染色体观察；初步观察细胞生理现象、细胞化学成分显示、染色体标本制备；掌握细胞培养、细胞冻存与复苏、细胞融合等技术的原理和方法；了解细胞放射自显影技术的原理和操作步骤；掌握实验报告的书写和镜下绘图的技能。

Methods HPDLFs were primary cultured from tissue explants, and the cells of the 5th to 8th passages were used after immunohistochemical identification of keratin and vimentin expressions. The cells were divided into 5 groups and treated with TP at 1, 0.5, 0.25, 0.125, and 0.0625 mg/ml, respectively, with another group without TP treatment as the blank control group. Cell counting and MTT colorimetric assay were performed to assess the cell proliferation, and flow cytometry was employed to determine the DNA content of the HPDLFs.

采用组织块培养法培养原代人牙周膜成纤维细胞并传代，经免疫组化SABC法检测角蛋白和波形丝蛋白鉴定，取5~8代细胞用于实验；按茶多酚不同浓度分1mg/ml、0.5mg/ml、0.25mg/ml、0.125mg/ml、0.0625mg/ml组和空白对照组，采用细胞计数法、MTT法检测细胞增殖情况，流式细胞术检测细胞DNA含量。

The aim of this study was to explore the potential relationship between the enhancement of instant hemostatic function in vivo of cryopreserved platelets and its procoagulative related molecule activities. The ability of platelet binding factor Ⅴ, density of GPIb-Ⅸ-Ⅴ(CD42a) at platelet member surface were detected by flow cytometry, the clotting time induced by activated platelets were evaluated by coagulometer and platelet count, MPⅤ and PDW were measured by hemocytometer before and after fresh platelets were cryopreserved.

为了探索血小板冰冻保存后膜表面促凝血活性相关分子变化与冰冻血小板体内即刻止凝血功能增强之间的可能联系，用流式细胞术检测了新鲜血小板冰冻保存前后Ⅴ因子结合能力、血小板膜表面GPIb-Ⅸ-Ⅴ分子(CD42a)密度，用血凝仪测定激活血小板诱导血浆凝固时间变化，用血细胞计数仪测定血小板计数、MPⅤ和PDW。

NHEK945 proliferation was assessed by MTT assay and direct cell count with a Coulter Counter.

人表皮细胞NHEK945增生采用MTT测定法和细胞计数仪直接计数法。

So, the nucleocytoplasmic ratio decreased. 10^(-5)MATRA significantly suppressed the proliferation of os732 with only 39.5% cells when compared with untreated cells. Cells in G1 phase increased significantly, and the number of S phase decreased. Expressions of cy-clinD1, cycimD2 and CDK4 mRNA declined, especially cyclinD2 and CDK However, expressions of cyclinD3 and P21WAF1 mRNA changed little.

结果 ATRA作用后，OS732细胞胞浆展开，细胞核变小，核浆比例变小；瘤细胞生长减慢，细胞计数为正常对照组的39.5%； G0/G1期细胞明显增多，S期细胞减少，细胞阻滞于G0/G1期；CyclinD1、CyclinD2、CDK4mRNA表达均下降，尤以CylinD2、CDK4下降最明显，CyclinD3、P21WAF1mRNA未见明显改变。

Methods The total and the differential cell counts with workstation and compared it to the chamber counting.

用工作站法进行脑脊液细胞计数和分类计数并与计数板法比较。

Human umbilical venous endothelial cells were cultured, and were exposed to different concerntrations of HCY with/or not with CuSO4; Cell counting of detached cells were performed with a hematocytometer; the cell survival was monitored by MTT assay and the cellular injury was evaluated by LDH release; Hoechst 33258 staining was used to observe nuclear morphological changes and the apoptosis was measured by DNA agarose gel electrophoresis ;the percentage of apoptosis was quantified by flow cytometry.

体外培养人脐静脉血管内皮细胞，用不同浓度的HCY伴／不伴生理浓度Cu~(2+)与内皮细胞作用；细胞计数板检测脱落细胞率；MTT法及乳酸脱氢酶释放率的测定来衡量细胞的存活与损伤；Hoechst 33258荧光染色观察细胞核形态变化和DNA琼脂糖凝胶电泳检测细胞凋亡，并采用流式细胞术定量测定细胞凋亡率。

Counting method of microorganisms in environment;②preparation of smears for observing forms and structures of bacteria、Actinomyces、yeasts and molds, methods and technologies of staining and oil immersion objective;③cell counting techniques and microscope examination techniques;④preparation of microbic media and sterilization techniques;⑤aseptic operations and microbic inoculation methods ;⑥technics of microbic separation、purification and cultivation;⑦physiological and biochemical reactions of microorganisms and the microbial examination techniques of food sanitation to strengthen students the operation techniques.

课程内容：本课程实验内容包括：①环境中微生物检测计数；②细菌、放线菌、酵母及霉菌细胞形态、结构的制片，染色和显微镜观测，油浸物镜的使用技术；③细胞计数技术和显微测量技术；④微生物培养基制备、压力蒸气灭菌技术；⑤无菌操作技术、移种技术；⑥微生物分离、纯化和培养技术；⑦微生物的生理、生化反应及食品卫生微生物检测技术等旨在加强学生微生物学的操作技术。

6 Days after plate culture, the cell number and cell viability of PLGA microspheres was much better than that of PELA microspheres and free bFGF.

4培养1天后，四组细胞计数、活力均无明显差异，4、6天PLGA组细胞计数和活力优于PELA组和游离bFGF组。

Immunohistochemical identification of cultured conjunctival cellsWhen the second cultured generation of conjunctival cells close to confluence, the glass coverslips were removed in situ fixation by methanol and DAB color immunohistochemical staining by CK13 monoclonal antibody in time.3. growth curve of conjunctival cellsThe 1st,3rd,5th passage cells were selectecd randomly and subcultured at the density of 5×10~4/ml,after culturing for another 1-9 days, were counted and compared the cell number every day.

采用消化后组织块法培养结膜原代细胞，并进行传代。2、培养结膜细胞的免疫组化鉴定把第2代的结膜细胞接种于置在培养皿中的盖玻片上，当细胞接近融合时，及时取出作原位甲醇固定，CK13单抗、DAB显色免疫组化染色。3、结膜细胞生长曲线的测定随机选取第1、3、5传代细胞，以5×10~4／ml密度传代、分别培养1-9天，每天分别细胞计数并进行分析比较，重复3次。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。