细胞计数

At 4 h, 1 d and 2 d after poisoned by mustard gas, the suspended bone marrow cells (106 cells per time) were injected into mice respectively. After poisoned 3, 5, 7 and 10 d, the numbers of leukocyte and marrow nucleated cells were recorded periodically.

染毒后4h、第1天和第2天3次尾静脉输注雄性小鼠骨髓细胞悬液（每次约1×10^6细胞），染毒后第3，5，7和10天取血和股骨，计数外周血白细胞和骨髓有核细胞。

The CNTF and FGF2 positive cell number and the positive area percentage, as well as the number of neuron and neuroglia calculated by immunofluorescence analysis in the spinal cord.

将嗅鞘细胞移植入脊髓半横断SD大鼠，观察术后7天和21天嗅鞘细胞在脊髓内的存活情况，并对脊髓组织切片进行CNTF和FGF2免疫荧光组织化学染色，计数阳性细胞并计算阳性面积百分比。

The extent of vesiculation was reversible along the time course .the mortality was low even under the constant presence of PCE for six days. At the end of the experiments ,the formerly vesicular cytoplasm was replaced with well-developed smooth endoplasmic reticulum ,a cytoskeletal system ,and numerous regeneration organelles .

取而代之的则是产生大规模的自我吞噬及发泡反应的现象﹐培养到第六天时﹐不但发泡反应几乎完全消失﹐细胞质中更且充斥了极发达的胞器﹐尤其是数量极多的平滑内质网﹐细胞骨架系统﹐以及不同型态之再生性胞器﹐另外在整个为时六天的PCE处理下的细胞之共同特徵是难以计数的胞饮小泡﹐表示细胞非常活跃地进行著物质交换。

The retina in Andrias davidianus were observed with light and scanning electron microscope.

用光镜和扫描电镜观察了大鲵视网膜各类细胞的形态及分布，对视细胞和节细胞进行计数。

Eight kittens were deduced amblyopia by monocular chronic atropinization; four (2 normal and 2 amblyopia) kittens were perused in every three weeks. Changes in number and optical density of immunopositive neurons of the expression of VIP-protin and VIP-mRNA in the visual cortex area 17 were analyzed with immuno-cytochemistry, using the technique of in situ hybridization and SABC.

在用药后3周、6周、9周、11周分别每组中各处死两只猫，分离出视皮质17区行HE染色观察神经元形态、细胞数目以及电镜观察超微结构变化，VIP-mRNA原位杂交和VIP免疫组织化学染色观察免疫阳性细胞数以及阳性细胞平均光密度，并分别用光镜下计数和计算机图形分析软件得出结果。

The laboratory findings of a typical CCML patient comprised of peripheral blood leukocytosis, basophilia and eosinophilia,myeloid differentiation in different stages, and increased megakaryocytes. The immunohistochemical features of the CCML consisted of highly positive MPO and CD68, significant lowering of neutrophil alkaline phosphatase, positive for Philadel- phia chromosome or chimeric BCR/ABL gene, etc. But in most cases of juvenile CCML, the Philadelphia chromosome could not be detected.

临床表现以乏力、低热、贫血、肝脾及淋巴结肿大为主要特点；典型儿童慢性髓细胞白血病的实验室特点有白细胞计数高、嗜酸和嗜碱性粒细胞增多、骨髓不同阶段髓细胞分化和巨核细胞增多；免疫组化提示CD68，髓过氧化物酶，中性粒细胞碱性磷酸酶积分明显减低，Ph染色体或BCR/ABL融合基因阳性，而幼年型CCML的Ph染色体常为阴性。

Currently, the common methods applied in the measurement of the total amount of bacteria include Plate Count method, Impedance method and Flow Cytometry method, etc.

目前用于快速菌群检测的主要方法有平板计数法、阻抗法和流式细胞仪计数法等。

Using Flow cytometer,TriTest reagent and Trucount tube(TriTEST/TruCOUNT)to assess absolute counts of CD 3,CD 4 and CD 8T lymphocyte subsets and CD 4/CD 8 ratio and observe T lymphocyte subsets distribution in sex,age,ethnics in the 110 healthy adults(Age∶16-56)in Guangxi.

用流式细胞仪(FACSCalibur，FCS)、三色免疫荧光试剂和绝对计数管(TnTEST/Tru COUNT)，检测 110名广西健康成年人(16～ 5 6岁)静脉全血CD3 、CD4、CD8T淋巴细胞绝对数值和CD4/CD8比值，并观察T淋巴细胞亚群分类计数在性别、年龄和民族上的差别。

The general information,including primary diseases,therapeutic drugs,infection pathogens in 6 patients with pulmouary infection and unknown reason anemia were integrated and analyzed. The leukocyte and reticulocyte counts,total bilirubin,direct bilirubin,fasting blood glucose were all determined by conventional method. At the same time,the erythrocyte direct antiglobulin test,complement fixation test,cell culture and smear examination were carried out.

对2004年6例肺部感染且不明原因贫血分析的患者的基础疾病、治疗用药、感染病原体等消息进行归纳，对患者外周血进行白细胞计数、网织红细胞计数、分胆红素、间接胆红素、血糖检测，对患者的红细胞进行间接抗人球蛋白试验、补体结合实验、细胞培养与涂片镜检，对患者血浆中的抗体进行间接抗人球蛋白试验。

The general information,including primary diseases,therapeutic drugs,infection pathogens in 6 patients with pulmouary infection and unknown reason anemia were integrated and analyzed. The leukocyte and reticulocyte counts,total bilirubin,direct bilirubin,fasting blood glucose were all determined by conventional method. At the same time,the erythrocyte direct antiglobulin test,complement fixation test,cell culture and smear examination were carried out.

对2004年6例肺部感染且不明原因贫血分析的患者的基础疾病、治疗用药、感染病原体等信息进行归纳，对患者外周血进行白细胞计数、网织红细胞计数、总胆红素、直接胆红素、血糖检测，对患者的红细胞进行直接抗人球蛋白试验、补体结合实验、细胞培养与涂片镜检，对患者血浆中的抗体进行间接抗人球蛋白试验。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐