细胞计数

The number of EOS in peripheral blood were counted by counting plate and the morphological changes in bronchia and EOS infiltration in lung tissue were observed. The content of NO and ET-1 in blood serum and BALF were measured by biochemistry and radioimmunity methods.

用计数板计数外周血嗜酸性细胞数量，观察小支气管形态学改变和肺组织中EOS浸润情况；生化法和放免法测定血清和肺泡灌洗液中一氧化氮和内皮素－1(ET-1)的含量。

Cell numbers were counted using the hemocytometer.

并使用血球计数盘来对细胞进行计数。

A549 cell line was stimulated with LPS (10 μg/mL) and then treated with Rs504393 (10 μg/mL) for 6 hours. ALI model was established with intranasal administration of LPS (5 mg/kg) in C57BL/6J mice. RS504393 (5 mg/kg) was administered 30 min before LPS dripped nasally. IL-8, IL-1β, plasminogen activator inhibitor-1, monocyte chemoattractant protein-2, and the expressions of CCR1 and CCR2b were studied by using Realtime-RT-PCR, ELISA and cyto-flowmetry.

体外实验选用A549细胞系，应用LPS(10μg/mL)刺激合并RS504393(10μg/mL)治疗6 h后，流式细胞仪技术检测CCR1和CCR2的表达，ELISA法检测细胞培养上清液内白介素-8的浓度；体内实验选用C57BL/6J小鼠，腹腔注射RS504393(5 mg/kg)预处理30 min后经鼻滴入JPS(5 mg/kg)，LPS刺激4 h后收集BALF和肺脏标本，计数BALF内细胞总数，应用BCA法检测BALF内蛋白浓度，Realtime-RT PCR和ELISA法检测BALF和肺脏IL-1β、凝血酶原激活物抑制物-1和单核细胞趋化蛋白-2的表达。

Oenococcus oeni was used as experimental material to count its number with three methods of colony counting method, nephelometer method and spectrophotometer method respectively.

以酒酒球菌为试验材料，分别用平板计数法、浊度计法、分光光度计法进行计数，所得的细胞数分别与对应的NTU值、OD值建立回归方程，方程分别为y=(0.0913+3.0075x)×107；y=(73.561x-4.8994)×107。

Beijing 100034,China(The School of Oncology,Beijing Institute for Cancer Research,Beijing Medical University,Beijing 100034,China)(The School of Oncology,Beijing Institute for Cancer Research,Beijing Medical University,Beijing 100034,China)Abstract : Objective In order to study the effects of ATP on U937 cells. Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used.

应用ATP(0.23g/L)作用于人白血病细胞U937，(1)用白细胞计数器每日计数细胞和计算抑制率；(2)用流式细胞分析仪文献检测 ATP对U937细胞周期改变和凋亡的影响；(3)苏木精-伊红染色和电子显微镜文献检测凋亡小体的形成过程；(4)用苯胺黑染色文献检测凋亡小体的生命本性。

One day post challenge, mice were measured via whole body plethysmography. Mice were sacrificed and bronchoalveolar lavage fluid was collected in the next day.

第3天剖杀，进行支气管肺泡灌洗、细胞总数计数、嗜酸粒细胞计数和肺组织炎症、支气管黏液分泌HE染色观察。

Result show that TRAP-positive mononucleated and multinucleated cells were markedly induced at the compression side at 3, 5, and 7 days. Mononucleated osteoclast reached peak level at 3 days after tooth movement, while multinucleated cells reached peak level at 5 days.

破骨细胞酶组化标记和计数统计显示，TRAP阳性细胞数量在牙移动第3、5和7天时与对照组比较有显著性差异；其中单核破骨细胞在第3天、多核破骨细胞在第5天时达到峰值。

Result: Schwann-like cells disperse in the hole of nerve graft at oneweek under Scanning Electronic Microscope, the processes of thesatellite-like cells adhere to the wall of graft.There was no statisticaldifference of activity of cells in both group(P＞0.05) using MTT. At 12weeks,the result of appearance, histology and TEM were similar, therewere no statistical difference in SFI%, NEP and quantitative analysis ofrecovery rate of myelinated fiber populations, diameter of myelinatedfiber and thickness of myelin sheat.

结果：1w后扫描电镜下可见接种的类Schwann细胞分散在材料表面的孔隙内，细胞形态为星状，细胞突起与支架管壁粘附；MTT法测定细胞活性示神经支架组在各时间点与对照组无显著性差异P＞0.05；12w时两组动物坐骨神经外观、组织学光镜观察、透射电镜观察结果相似、两组坐骨神经功能指数恢复率、神经电生理、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率等指标相比无显著性差异(P＞0.05)。

The adipose derived stem cells of inbreds train of rat wereinduced to differentiate into schwann-like cells and the cells were injectedinto the acellular nerve allograff.The proliferation or adhersion of these cells on the graft were observed by inverted microscope or ScanningElectronic Microscope. Activity of cells were detected using MTT. 10mmnerve gap of inbreds train of rats in two group were bridged by tissue-engineered peripheral nerve or autogenous nerve, the effect wereappraised by naked eye, recovery rate of sciatic function index,nerve-electrophysiological, histology, Transmission ElectronMicroscopy and quantitative analysis of recovery rate of myelinated fiberpopulations,diameter of myelinated fiber and thickness of myelin sheat.

按照前述方法培养并诱导近交系大鼠脂肪干细胞向类Sehwann分化，将诱导分化的脂肪干细胞悬液注入已制备的去细胞同种异体神经支架管中，倒置显微镜观察细胞生长情况；扫描电镜下观察细胞在材料上附着情况；四唑盐比色试验测定细胞活性；取两组近交系大鼠，分别用组织工程化神经和自体神经桥接10mm神经缺损，通过大体观察、坐骨神经功能指数恢复率的测定、神经电生理的测定、组织学光镜观察、透射电镜观察、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率的测定等指标评价实验效果。

The culture was treated with lutein of different concentrations 10^(-8mol/L、10^(-7)mol/L、10^(-6)mol/L, respectively. The culture cells were fixed and were stained for tartate-resistant acidic phosphatase after 7d. The formation of osteoclasts was quantified by counting the number of TRAP(superscript +) multinuclear cells. The percentage of bone resorbed surface, TRAP activity and the expression of receptor activator of NF-KB mRNA were analysed. The appearance of reduced mice osteoclasts was typical.

受试细胞分为对照组、叶黄素低剂量组10^(-8mol/L、叶黄素中剂量组10^(-7mol/L和叶黄素高剂量组10^(-6mol/L，并设立空白对照组。7 d后取细胞玻片进行抗酒石酸酸性磷酸酶染色，观察破骨细胞并计数；取骨片进行甲苯胺蓝染色，光镜下统计骨吸收陷窝面积；测量抗酒石酸酸性磷酸酶活性以及破骨细胞表面NF-κB活化受体mRNA表达量。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐