细胞计数

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗；(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力；(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性；(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡；(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型，应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况，明确其靶向性；(6)将荷瘤裸鼠分3组进行放射免疫治疗，分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

Methods: Human colon cancer cell line DLD1 was treated with Bcl-XL siRNA, The protein level of Bcl-XL in the cells was determined by Western blot; the apoptotic ratio and survival condition were detected by flow cytometry assay and trypan blue-stained cell counting with hemocytometer, respectively. Results: The level of Bcl-XL protein in DLD1 cells was obviously down-regulated by Bcl-XL siRNA.

采用人结肠癌细胞株DLD1，利用人工合成的Bcl-XL靶向小分子干扰RNA转染DLD1细胞，通过Western blot法检测转染细胞Bcl-XL蛋白的表达，流式细胞仪检测处理后细胞的凋亡情况，锥虫蓝染色法计数存活细胞。

The cells were incubated in RPMI 1640 without or with different contents of glutamine and with 10%fetal calf sera, at 37℃,5% CO2 and saturation humidity for 4 days.The initial living cell density was 5×108/L. After 4 days incubation,the cells were counted,collected, smeard and stained with Wright-Giemsa, DNA, POX, NAE and NaF inhibition,gulping ink and NBT etc. cytochemical technology. The stained cells were investigated under oil immersion lens.

从18例未经治疗的APL病人外周血中提取APL原代细胞，在无Gln的RPMI 1640培养液中补加10%胎牛血清，以活细胞密度5×108/L接种细胞，在37 ℃， 5%CO2和饱和湿度下培养4 d，计数活细胞，并收集细胞行Wright-Giemsa、DNA、POX、NAE及NaF抑制试验、墨汁吞噬试验和NBT还原试验等细胞化学染色，于油镜下观察。

Methods: Separate and abstract the effective components of Huangqi (Omni-Saponin、total-flavone) and Danshen (Danshen-ketone、 Danshen-Quinone). Applying L-Arginine methyl ester to pregnant rats by I. P to make Nitrogen Oxide Synthesis blocking models (uterus-placenta-fetus lacking blood and oxygen model or pregnant hypertension syndrome model). On day 12, 18 of pregnancy paunched the rats and took out the fetus and placenta/uterus. Measured the basal BP during pregnant period and BP、protein、basal activity each day. Observed placenta/deciduas ultramicroscopic structure by electron microscope and took count of languishment cells. Measured the expressive intensity of bcl-2、Bax of placental nutrient cells by SABC method.

一氧化氮合成酶阻滞剂L-精氨酸甲酯妊娠大鼠腹腔给药，制作一氧化氮合成阻滞模型（子宫-胎盘-胎儿缺血缺氧或妊娠高血压模型），妊娠第12、18天剖腹取胎、胎盘／子宫，妊娠期测基础血压，每日测血压、蛋白质量、基本生理活动；电镜观察胎盘／蜕膜超微形态结构，并进行细胞凋亡计数；免疫组化检测胎盘滋养细胞bcl-2、Bax表达强度；原位杂交法检测胎盘滋养细胞、血管内皮细胞VEGF、eNOS、iNOS、MCP-1、MMP9（基质金属蛋白酶9）mRNA表达。

The survival time of these mice were observed. The count of white blood cells and the percentage of immature granulocyte and monocyte obtained from tail vein were determined on 1 day before transplanting WEHI-3 cells and 7, 14, 19, 24, 28 days after transplanting. Myeloblast and monoblast percentage of bone marrow were checked as soon as these mice died.

BALB/c小鼠分别经尾静脉和球后接种1×10^6 WEHI-3细胞，观测其生存天数，光镜监测BALB/c小鼠外周血白细胞计数、未成熟粒单核细胞百分率及骨髓原始粒单核细胞百分率，电镜观测其骨髓白血病细胞形态。

The concentration of diluted rv virus solution was determined by counting the nih3t3 cells with fluorescent expression. then the rv/egfp virus was used to transfer sk n sh neuroblastoma cells. the transfection efficiency in sk n sh cell was measured by flow cytometer.

将稀释的rv病毒液感染nih3t3细胞，计数nih3t3荧光表达细胞的数量来确定病毒的浓度；使用rv载体感染sk n sh细胞，通过流式细胞学荧光检测明确rv在sk n sh细胞的感染效率。

The CCND2 expression cell proliferation, cell cycle and cell apopotosis of the transfected LP-1 cells were studied by RT-PCR, trypanosome staining, flow cytometry and annexin V assay.

设计、合成CCND2短发夹RNA双链DNA模板，构建表达质粒pGenesil-2／CCND2，转染LP-1细胞，以RT—PCR检测对CCND2表达的抑制作用，以锥虫蓝染色计数和MTI检测细胞增殖，以Annexin V检测细胞凋亡，流式细胞术检测细胞周期。

Methods: Viable cell numbers and viability were counted by means of trypan－blue exclusion.

在台盼蓝排除法计数活细胞和细胞活力的基础上，通过细胞形态学和流式细胞仪检测细胞凋亡。

The uptake of 〓I-OxLDL in adipocytes was determined by gamma ray counter and normalized to protein concentration of each sample, was expressed as nanograms of 〓I-OxLDL uptake per milligram of cell protein. Reverse transcription polymerase chain reaction was used to evaluate PPARγ and CD36 mRNA expressions.

采用酶联免疫吸附法检测血浆IL-6以及脂肪细胞分泌IL-6的水平；采用gamma射线计数仪来检测脂肪细胞对〓I-OxLDL的特异性摄取情况，采用改良Lowry法进行细胞蛋白浓度测定，细胞对〓I-OxLDL的特异性摄取量以ng／mg细胞蛋白表示；采用逆转录聚合酶链式反应来检测PPAR γ和CD36 mRNA在脂肪细胞的表达情况。

At the same time, group B cells were sequential planted by allowing cells to adhere to tissue culture dishes during two successive 2-hour incubations in order to remove non-adherent cells.

分别采用常规方法和序列黏附法培养。A组细胞于接种第4天洗去未黏附细胞，然后隔d换液1次；B组细胞在接种后每2h去除1次未黏附细胞，共2次。2组细胞均在第7天计数早期克隆，持续培养直到晚期克隆出现。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐