细胞计数

Objective To count the number of ribbon synapses in cochlear inner hair cell using three-dimensional reconstruction model.

目的利用三维建模的方法对耳蜗内毛细胞带状突触进行计数分析，解决由于内毛细胞带状突触数量少、位置深在造成的计数困难，为内毛细胞带状突触的可塑性研究提供有效可靠的计数方法。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

Immunohistochemistrical section: observe the VEGF protein expression in different cells, the positive ratio detected by a morphometrical analysis system was used as the amount of the VEGF protein expression.

免疫组化染色切片：观察不同给药时间股骨头内VEGF的表达情况；高倍视野下（400倍）沿骨小梁表面计数50个成骨细胞中的阳性细胞数，VEGF成骨细胞阳性率=阳性成骨细胞数/50×100％，3次取平均值；计数每个高倍视野软骨细胞中的阳性细胞数，VEGF软骨细胞阳性率=阳性软骨细胞数/软骨细胞总数×100％，5次取平均值。

Methods: Forty mice were divided into four groups randomly:normal group,model group,ligation group and treatment group.The model was made by the method of partly ligating the recta outside the body.The level of endotoxin in blood plasma was measured by dynamic nephelometric method and the content of TNF-a in blood serum was detected by the method of radioimmunity.(2)After the mice were killed,their left lungs were taken out for bronchi-alveoli fluid, then the content of MDA in BALF was tested by TBA method.(3)The vitality of PAM in BALF was countied on the counting set of erythrocyte,and the blue-dyed cellswere counted, then the percentage of PMN was worked out.(4)The pathological features of the mice\'s lung tissue were observed with routine and ultramicro pathological mechanism.

①将40只大鼠随机等分为正常组、模型组、解扎组、治疗组，以体外直肠不全结扎法造模，以动态浊度法检测各组大鼠血浆内毒素水平，放射免疫分析法检测各组血清TNF-α含量；②大鼠处死后，取左肺进行支气管肺泡灌洗，以TBA法测定支气管肺泡灌洗液中丙二醛含量、白蛋白含量及肺表面活性物质水平；③在红细胞计数盘上作BALF中肺泡巨噬细胞活力计数，计数兰染的细胞数，求出死亡百分率；在光镜下计数BALF沉渣200个细胞，计算中性粒细胞所占百分比。

So in one way IGF can actually change your genetic capabilities in terms of muscle tissue and cell count.

因此，在胰岛素样生长因子的一种方式实际上可以变更您的遗传能力方面的肌肉组织和细胞计数。

SCM adhibits CCD image cell as the counting scenery position.

单片机adhibitsCCD图像作为细胞计数风光位置。

Methods This study consisted of 110 women with 8-40 weeks of pregnancy (60 with normal pregnancy, 30 with pathopregnancy, and 20 with fetal disorder). Counting of FNRBC in the maternal blood and its relationship with gestational weeks, pathological gestation and abnormal fetus were evaluated by single density centrifugation and Wrights staining.

方法对110例8～40孕周的妇女(60例正常妊娠，30例病理妊娠，20例胎儿异常)外周血进行单密度梯度离心，瑞氏染色和细胞计数，分析母血中FNRBC数量在病理妊娠和胎儿异常时的变化及其与孕周的关系。

Didanosine, and Nevirapine combined therapy for one year. All cases were followed up. After been treated 3 months, 6months, 9months and 12 months, the plasma HIV-l RNA level and CD4(superscript +)T cell count of the patient were measured at beginning of treatment, the third month, the sixth month, the ninth month, and the twelfths month.

选择符合抗病毒治疗条件的艾滋病9例，给予口服司他夫定＋去羟肌苷散＋奈韦拉平一年，在治疗前及治疗后第3，6，9，12月分别检测血中HIV RNA病毒载量、CD4T细胞计数等。

After been treated 3 months, 6months, 9months and 12 months, the plasma HIV-1 RNA level and CD4^＋T cell count of the patient were measured at beginning of treatment, the third month, the sixth month, the ninth month, and the twelfths month.

选择符合抗病毒治疗条件的艾滋病9例，给予口服司他夫定＋去羟肌苷散＋奈韦拉平一年，在治疗前及治疗后第3，6，9，12月分别检测血中HIV RNA病毒载量、CD4^＋T细胞计数等。

WBC count and classification, lung X-ray, acidophil sorting in sputum and serum IgE were tested for all of the patients.

检测每组的外周血WBC及分类，胸片，痰中嗜酸性细胞计数，血清IgE定量。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。