细胞计数

Objective To investigate the correlation between the conspicuity of local hepatic lesions on feridexs-enhanced MR images and the number of Kupffer cells in the hepatic lesions in histopathologic findings, as compared with that in the background liver.

结果良、恶性肝内局灶性病变间Kupffer细胞比率差异有统计学意义，随着HCC分化程度的降低，病灶内Kupffer细胞计数比率呈下降趋势(P05)。T2WI强化比率与Kupffer细胞计数比率呈负相关(r=-0780，P05)；T1WI强化比率与Kupffer细胞计数比率呈正相关(r=0389，P05)。

Results There were significant differences between MDS and CAA in Hb, red cell distribution width-coefficient variation, immature reticulocyte fraction, BPC, the ratio of G1 (the sum percentage of myeloblast and premyelocyte) to G2 (the sum percentage of neutrophilic myelocyte and metamy-elocyte), the ratio of E1 (the sum percentage of proerythroblast and early erythroblast) to E2 (the sum percentage of intermediate erythroblast and late erythroblast), megakaryocyte count,erythroblast PAS, neutrophil alkaline phosphatase, and serous levels of indirect bilirubin,lactose dehydrogenase, folic acid, VitBl2 and ferritin.

结果 MDS患者血红蛋白，红细胞体积分布宽度。变异系数、未成熟网织细胞比率、血小板计数、骨髓原始细胞及早幼粒细胞之和与中性中幼粒细胞及中性晚幼粒细胞之和的比值、原始红细胞及早幼红细胞之和与中幼红细胞及晚幼红细胞之和的比值、巨核细胞计数、有核红细胞糖原染色阳性率和阳性指数、中性粒细胞碱性磷酸酶染色阳性率和阳性指数、血清间接胆红素、乳酸脱氢酶、尿酸、叶酸、维生素B12(VitB12)、铁蛋白水平等常规实验室指标与CAA患者比较差异有统计学意义。

Combined with the data of cell density by hemocytometer counting or MTT staining, the equations that account for the dependence of apoptotic/necrotic percentages on the fluorescence intensities of YP/PI were derived.

结合细胞密度的计数结果(细胞计数板、MTT比色法等)，建立了用YP、PI荧光强度值计算细胞凋亡和坏死百分数的方法。

Quiescent RASMCs were incubated respectively in 10% FBS DMEM as control group, 10% FBS DMEM supplemented with 0.1% dimethyl sulfide as vehicle group and 10% FBS DMEM supplemented with panaxynol(at concentration of 1,3,9×10-6 mol·L-1) as panaxynol groups. 1. Cell counting and double time. Cell viability was examined with trypan blue exclusion test and the cell number was counted at 0, 24, 48, 72h after the incubation using a hemocytometer.

实验中细胞分成如下几个处理组：①正常对照组：含10％FBS的培养液；②溶剂对照组：0.1％DMSO；③药物处理组：panaxynol 1、3、9×10-6 mol·L-1组。1、细胞计数及倍增时间测定：于接种并同步化后处理0、24、48、72h，用血细胞计数板计算活细胞数量，绘制生长曲线并计算细胞倍增时间。

According to the bone apposition events at the interface, we measured the osteoblast attachment rate with the hemocytometer, the osteoblast growth curve with modified MTT method, the ALPase activity and protein content of cellular layers with modified kinetic method and modified Coomassie'method respectively, and osteocalain content in the cell medium with RIA , at different levels of cell responses, as osteocompatibility indexs.

根据骨内种植体界面的骨愈合过程，分别从成骨细胞反应的不同层次选择了细胞贴壁率、细胞生长曲线、细胞层ALPase活性和蛋白质含量以及细胞培养液中骨钙素的含量作为体外评价材料骨性生物相容性的指标。采用细胞计数板计数法测定细胞贴壁率，采用改良MTT法测定细胞生长曲线。

The expression of VEGF was observed by using image analysis and the secretion of VEGF by macrophagus was tested by using immunohistochemistry method.

结果：在口腔鳞癌中，VEGF的表达和微血管计数、巨噬细胞计数呈现正相关(P.05)；VEGF的表达、巨噬细胞的浸润和肿瘤的淋巴结转移有关(P.05)，口腔癌浸润的巨噬细胞中有29%~10%的细胞内有VEGF的表达。

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

Pyriformis cells cultivated in liquid complex culture media containing 0, 0.05, 0.25, 0.5, 2.5, and 5 mg/mL of melamine were plotted by recording the number of cells present in the culture medium during the course of a 52-hour cell growth experiment, with a strictly controlled time interval of 4 hours.

本实验利用倒置光学(来源：A83B38f6C论文网www.abclunwen.com)显微镜下血球计数板计数法进行细胞计数，每两次细胞计数之间的时间间隔为4小时。

Blood samples were drawn from every patient at admission to the detect the leukocyte counts in peripheral blood, including total leukocyte count, neutrophil count, eosinophil count, lymphocyte count, monocyte count and basophil count.

对所有患者进行人体测量(体质量指数与收缩压、舒张压的测量)，检测患者的外周血中白细胞计数(包括白细胞总数、中性粒细胞计数、嗜酸性粒细胞计数、淋巴细胞计数、单核细胞计数和嗜碱性粒细胞计数)。

Methods After Candida albicans were inoculated into the specific pathogen free mice, the total amount of Candidas in cecum and the amount of which attached to the mucosal membrane were counted at different interent intervals. The lymphocyte proliferation in Peyer's patch and in lamina proper was shown by BrdU incorporation, meanwhile B cells isotype switch in PP was investigated. IgA plasma cells were shown by immunohistochemical staining. Specific IgA antibodies to Candida albicans were measured with ELISA.

采用无特殊病原菌动物经口喂入白念菌，在不同时相处死后，观察肠内白念菌总数及肠粘膜表面白念菌粘附数量；取肠系膜淋巴结做白念菌选择培养，观察移位体内发生率；采用Brdu体内掺入显示局部粘膜淋巴细胞的增殖情况；流式细胞计数潘伊尔氏结细胞表面IgA阳性率；免疫组化染色后计数固有层中IgA浆细胞数量变化；ELISA法测定肠粘液中特异抗白念菌IgA含量。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。