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细胞色素

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We got the result: Bufo Raddei Strauch Tadpole (1) Bufo Raddei Strauch Tadpole was capable of lens regeneration, which originated from the epithelial cells at iris dorsal margin by dipigmentation.(2) Dipigmentation of Bufo Raddei Strauch by two methods: one was licked up by amoebocyte, the other one was flowed from the pigment cells.(3) In the lens regeneration cells of Bufo Raddei Strauch profiles of the mitochondria became complicated ribosome manifold, and the endoplasmic reticulum was cisternal dilation. During the process of deferentiation of lens fiber the cell nucleolus became little and chromatin shrinked.(4)The crystalline electrophoresis of Bufo Raddei Strauch Tadpole lens regeneration showed that during lens regeneration P-crystalline was expressed earliest then was γ-crystalline and α-crystallin, this result is consistent with newt.

结果表明:花背蟾蜍蝌蚪(1)具有晶状体再生的能力,其晶状体的再生来源于虹膜背缘的上皮细胞;(2)虹膜背缘去色素是转分化的前提,主要有两种方式:一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞,巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒;另一种方式是虹膜色素上皮细胞自身释放色素颗粒;(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化,核糖体增多,粗面内质网增多,晶状体纤维分化过程中细胞核逐渐凝聚;(4)SDS-PAGE电泳发现,花背蟾蜍蝌蚪晶状体再生中相关蛋白表达顺序与有尾类蝾螈相似,蛋白表达顺序依次是β-晶状体蛋白、γ-晶状体蛋白和α-晶状体蛋白,并且后两种蛋白几乎同时表达。

ResultsYAG laser could healing the pigment pathological changes, chromatophore present vacuolus.But the Vallate frame remaining Integrity.The surrounding tissue of chromatophore have not injury and still survival. Macrophage was heightened, pigment granule appear in the cell.

结果:YAG激光治疗色素病变可以消失,色素细胞呈现空泡状,但色素细胞轮廓的框架仍可完整保留,色素细胞周围组织并未受损伤仍可存活,并可见巨噬细胞增多,该细胞内出现色素颗粒。

Its main features are: algae body as a single cell or even into a variety of groups; cell walls composed of silica and pectin, but also by the structure of the shell and the shell under the combined set of the Cheng, vertical section view was "soap-box"-shaped; in the upper and lower shell surface of the shell are patterns arranged in patterns on both sides of the main mode of symmetry and radial symmetry is divided into two categories, for the classification of the most important basis; be able to campaign Type in the shell has a shell side seam, can not be changed no shell seam types; pigment body sheet or ribbon, 1 or 2, or for multiple small discoid, yellow green or brown, containing chlorophyll a, c, also contains fucoxanthin and silicon A flavin and other photosynthetic pigments; storage material mainly for oil droplets; reproduction mainly for cell division and cell division in diatoms obvious features are: each cell division, resulting from two sub-cells,, only one and the mother cell and so big, the other one is smaller.

其主要特征是:藻体为单细胞或连成各式群体;细胞壁由硅质和果胶质组成,而且在结构上都是由上壳和下壳套合而成,纵断面观呈"肥皂盒"形;在上下壳的壳面上有花纹,花纹排列的方式主要分为两侧对称和辐射对称两大类,为分类上的最重要依据;能运动的种类在壳面都有壳缝,不能动的种类均无壳缝;色素体片状或带状,1个或2个,或为多个小盘状,黄绿色或黄褐色,含叶绿素a、c,还含有墨角藻黄素和硅甲黄素等光合色素;贮藏物质主要为油滴;繁殖方式主要为细胞分裂,硅藻的细胞分裂的明显特点是:每次细胞分裂所产生的2个子细胞中,仅有1个和母细胞等大,另1个则稍小。

The results shown that:(1) Bufo raddei Strauch tadpole was capable of lens regeneration, which originated from the epithelial cells at dorsal iris margin by depigmentation.(2) Depigmentation of Bufo raddei Strauch during lens regeneration was by two methods: First, pigment granules initially dispersed all over the cytoplasm were moved towards the periphery of the cell, and then were directly taken up by the amoeboid cells. Second, pigment granules in a cell were first crowded in a mass, surrounded by a membranous structure in the cytoplasm, which were eventually discharged as a mass from the cell.

结果表明:花背蟾蜍蝌蚪(1)具有晶状体再生的能力,其晶状体的再生来源于虹膜背缘的上皮细胞;(2)虹膜背缘去色素是转分化的前提,主要有两种方式:一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞,巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒;另一种方式是虹膜色素上皮细胞自身释放色素颗粒;(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化,核糖体增多,粗面内质网增多,晶状体纤维分化过程中细胞核逐渐凝聚。

The cilia of retinal pig merit epithelium disappeared completely by TEM, the granola of retinal pigment epithelium decreased, the rough endoplasmic retinal, the mitochondrial crista breaks, the outer nuclear layer arrangemen disorderin, disc broad, the vacuole timer and outer plexi-form layer shaped, the ganglion cells and membranous of eye cell outer side got think, intermembranous space gotorgancelle disappeared mostly, the crista of cytoplasm ganglion cells layer swelled.

透射电说可见:视网膜色素上皮细胞表面纤毛完全消失,视网膜色素上皮细胞内颗粒减少,粗而内质网、线粒体嵴断裂,外颗粒层细胞排列紊乱,视细胞外段盘膜粗大,盘膜间隙增宽,内外丛状层空泡形成,神经节细胞、细胞器大部分消失,神经节细胞层可见细胞质有嵴性肿胀。

RESULTS: In the retinal region without light, the structure of every layer was clear, cells in neuroepithelial layer arrayed in rule, some bubble presented in external granular layer and internal granular layer, RPE cells were compact, and the color of pigment article was coincident. In the region with direct blue light and that with 30J/cm2+Acrysof one-piece/ pMMlA, cells on photoreceptor and external granular layer were lost partially, bubble increased, RPE cells were with different sizes, and cell edema, cell lost and pigment article cluster could be seen.. In region with 30J/cm2+Natural, a little disorganization could be seen comparing to that without light, but more normal than those with Acrysof and direct eradia tion.

结果:未照射部位:视网膜各层次结构清晰,神经上皮层内各细胞排列规则,整齐,RPE细胞排列紧密均匀,色素颜色一致;单独光照和透过Acrysof一片式I0L:光感受器层部分细胞丢失,外核层可见固缩核,浓密,内颗粒层细胞大量丢失,内丛状层内空泡明显增多,RPE表面,细胞大小不均,可见肿胀、缺失,细胞内色素浓密、聚集成块;透过Nat-ural一片:与未照射处相比,视网膜各层次结构仍有部分破坏,但程度明显减轻。

In nature, the core structure of chlorophyll, haemachrome, cytochrome, and other biological macromolecules are made up of various met-alloporphyrins. The MPs can participate in a series of important processes in the organism, especially in the transfer processes of messenger gaseous molecules.

在自然界,金属卟啉配合物构成了叶绿素、血红蛋白、及细胞色素等生物大分子的核心部分,参与生物体内信使气体分子的体内传输过程。

On the basis of the previous research work of our lab, the PAMAM/HA composition coatings have been deposited on the electrodes by continuous potential step method, to study the electrochemical behaviors of the biological molecule such as cytochrome c, glucoseoxidase, haemachrome.

本文在实验室已有的对HA电沉积研究的基础上,应用双电位阶跃法在铂电极和玻碳电极上电沉积制备羟基磷灰石涂层,考察细胞色素C、葡萄糖氧化酶、血红蛋白等生物大分子在HA修饰电极上的电化学行为。

We found, on the one hand, urea can cause cytochrome cboth on the electrode surface and in the solution to be denatured and then the denaturedprotein lends to move the formal potential of the native protein in the bulk solution to amore negative value; and on the other hand, urea can also cause the formal potential ofnative protein in the bulk solution to shift positively with decreasing the degrees ofdissociation of electrolytes and polarity of the urea-water solvent.

我们发现脲能引起电极表面和溶液中的细胞色素C的变性,变性蛋白质能导致本体溶液中天然蛋白质的标准式电位的负移;另一方面,通过降低电解质的离解度和脲-水溶剂的极性,脲能使本体溶液中天然蛋白质的标准式电位正移。

It was shown from electrochemical experiments that cytochrome cexhibited direct electrochemical responses on the both electrodes, but only the currentof diffusion existed on the SWNT/Au electrode while both the current of diffusionand adsorption of cytochrome c occurred on the SWNT/AET/Au electfode.

实验结果表明,细胞色素C在这两种电极上均表现出良好的电化学响应,但在SWNT/Au电极上只存在扩散电流,而在SWNT/AET/Au电极上同时表现出扩散和吸附两种电流。

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