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The results showed that after treatment of neural stem cells by RA, apoptosis factor cytochrome c was released.

同时应用Western blot检测RA诱导神经干细胞凋亡过程中细胞色素C的变化,结果发现在细胞质中有细胞色素C的释放。

Preliminary standard reference spectra for the unliganded enzyme and for the spectral changes induced by sodium dithionite and succinate were obtained. The wavelength and intensity of the UV signal observed in agreement with results of ox-reduced state of heme b.

其次我们使用不同还原剂succinate、Na2S2SO3分别处理两种纯度的样品,通过分光光度计检测细胞色素b的氧化还原状态,观测到了不同还原剂影响下的细胞色素b的特征光谱。

By employing two kinds of molecular marker of mitochondrial DNA, i. e. Cyt b and CoI gene, the sequences of 15 species representing 6 families from Passeriformes were compared. We analysed their sequences compose and characteristic with the sorft of MEG3.1, and built the phylogenetic trees in PAUP~*4.0. We deeply analysed the feasibility that CoI gene qua the marker to research the phylogenetic relationship of Passeriformes, and afford the academic proof for building the DNA barcoding of the birds.

本文分别以线粒体DNA的细胞色素b和CoI基因作为分子标记,对雀形目6科15种鸟类的细胞色素b基因全序列(1,143bp)和CoI基因部分序列(1,176bp)的组成特点及其系统发育树进行了比较分析,还探讨了CoI基因作为雀形目系统发育研究和构建鸟类物种DNA编码库的分子标记的可行性,旨在为DNA编码库的构建积累基础资料。

Mainly using protein - film voltammetric technique, we have achieved direct electrochemical response of lots of proteins such as hemoglobin, myoglobin, cytocrome c, cytocrome b5, horseradish peroxiase, GOD ,Vitreoscillia sp. haemoglobin, etc., at a series of protein membrane modified electrodes, for instance, protein/kieselgubr, protein/polyethylene glycol, protein/polyethyleneimine, protein/phosphatidylcholine, protein/DNA, protein/montmorillonite/polyvinyl alcohol, protein/silver nanoparticle/ cysteamine membrane modified electrode, etc., which are prepared by casting, incorperating or layer by layer self-assembly methods. Electrochemical behavior of these proteins in the films have been studied, and their structural and conformational changes have also been examined with spectrometric techniques.

三年来,在基金委的支持下,我们以蛋白膜伏安法这一新技术作为主要的研究手段,并结合其他生物、化学技术,研究了血红蛋白、肌红蛋白、细胞色素C、细胞色素b5、辣根过氧化物酶、葡萄糖氧化酶以及好氧革兰式阴性菌透明颤菌中的血红素类蛋白等多种蛋白质在以涂布和自组装方法制成的蛋白质/硅藻土膜、蛋白质/聚乙二醇膜、蛋白质/卵磷脂膜、蛋白质/聚乙烯亚胺膜、蛋白质/蒙托石/聚乙烯醇自组装膜以及蛋白质/银胶/半胱胺自组装膜等修饰电极上的直接电化学行为,结合各种光谱技术,对蛋白质在膜中的构象进行表征,探索其电子传递机理。

By using of the Tripos sybyl software, three-dimensional(3D) model of p450nor2 was established based on high sequence similarity to homologous protein p450nor1 structure and the perfect homology modeling was obtained.

基于细胞色素P450nor2与同源蛋白P450nor1氨基酸序列的相似性,采用Tripos sybyl软件对细胞色素P450nor2进行同源模建和分子叠合,获得了理想的同源模建结果。

Mitochondrial cytochrome b gene were sequenced in 44 geese of Chinese domestic geese(15 breeds) and European domestic geese(2 breeds). Anatidae molecular clocks was calibrated with 2 consensus sequences which come from Chinese domestic geese and European domestic geese and 13 sequences obtained from GenBank.

本实验测定了中国家鹅15个品种、欧洲鹅2个品种共44个个体的细胞色素b基因全序列,分别取鸿雁和灰雁家鹅的一致序列与GenBank库中的13条鸟类(鸡形目4条、鸭科鸟类9条)细胞色素b基因部分序列(1045bp)共15条序列构成本研究的数据集。

The bacterial, mammalian and plant cytochrome P450s involved in metabolism of herbicides are reviewed in this paper. The action modes of cytochrome P450s on herbicides including dealkylation, ring-methyl hydroxylation and aromatic ring hydroxylation etc are summarized.

本文综述了代谢除草剂的细菌、哺乳动物和植物细胞色素P450酶系,概述了细胞色素P450酶系参与除草剂代谢的作用方式:脱烷基化作用、环甲基化羟基化作用和芳环的羟基化作用等。

PartⅢEffect of DM on release of cytochrome C in neurocyte mitochondria Objectivity: To study the effect of DM on release of cytochrome C in neurocyte mitochondria.

第三部分溴氰菊酯对神经细胞线粒体细胞色素C释放的影响目的:探讨溴氰菊酯对神经细胞线粒体细胞色素C释放的影响。

Long period illumination could lower the activity of cytochrome oxidase in tissues, so that this enzyme activity in green leaves was much lower than that in etiolated leaves. The activity of isolated cytochrome oxidase, however, was not affected with light of varied intensities.

虽然光的长期作用能降低细胞色素氧化酶活性,使绿叶中该酶的活性大大低于黄化苗,但各种强度的光对提取的细胞色素氧化酶活性没有影响。

Measurement of cytochrome oxidase activity in germinated seeds, etiolated leaves and green leaves of pea indicated that the cytochrome oxidase activity was correlative to the importance of energy generation in the process of mitochondrial coupling respiration.

豌豆萌发种子、黄化苗和绿叶的细胞色素氧化酶活性测定表明,细胞色素氧化酶活性在黄化苗、萌发种子、绿叶中依次递减,与线粒体偶联呼吸产生能量在该组织中的重要性呈正相关。

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