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细胞色素

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Because of sharing characteristics with both Galliform groups, Tragopans are classified as partridges by some authors and pheasants by some others. In this study we set out to sequence the cytochrome b-gene of 19 species pheasants. and took the sequence for those 22 species from GenBank. We amplified 828 base pairs using the PCR technique. Primers designed by ourselves were based upon Galliform sequence data. Neighbor-joining method and Minimum evolution method were used to analyse cytochrome b sequences with Anhima cornuta and Kachuga dhongoka as outgroups.

通过PCR扩增鸡形目Galliformes黄腹角雉Tragopan caboti等10个属19个个体的线粒体DNA细胞色素b的部分基因,获取片段长度为828bP,以及从GenBank获取22种样本的相应序列,以角叫鸭Anhima cornuta和海龟Kachuga dhongoka为外群,分别用邻接法(Neighbor-joining, NJ)和最小进化法(Minimum-evolution, ME),对鸡形目和将雉科的15个属分为5个属及雉族和鹑族构建分子系统树,NJ和ME系统树中,都是角雉与雉类相聚。

AIM: To determine the distribution and frequency of functionally important allelic variants in the cytochromes P450 2C19, arylamine N-acetyltransferase 2 (NAT2), and thiopurine S-methyltransferase genes in the Han Chinese population and compare them with those of other ethnic populations.

目的: 了解细胞色素P450(cytochromes P450,CYP)2C19,N-乙酰基转移酶2(arylamine N-acetyltransferase 2,NAT2)和硫嘌呤甲基转移酶(thiopurine S-methyltransferase,TPMT)基因常见的遗传多态性在河南地区汉族人群中的分布及其频率。

In this paper, the cloning, expression and phylogenetic analysis of AmphiSDHD and AmphiS27 from the gut cDNA library of amphioxus Branchiostoma becheri tisingtauense are carried out.AmphiSDHD, encoding the cytochrome b small subunit in mitochondrial succinate-ubiquinone oxidoreductase, was isolated from the gut cDNA library of amphioxus Branchiostoma belcheri tsingtauense.

本文从青岛文昌鱼(Branchiostoma belcheri tsingtaunese)肠cDNA文库中,分离得到克隆L095和094,经鉴定它们分别编码文昌鱼线粒体琥珀酸-泛醌氧化还原酶中细胞色素b的小亚基和核糖体蛋白S27(AmphiS27)。

The present study eatablished genechip technique for Ceratitis capitata, C. rosa and C. cosyra, in which the mitochondrial cytochrome oxidase Ⅰ gene was used as molecular marker, and the DNA sequences of fruit flies (Diptera: Tephritidae) were used as target sequence. The genechip detection system for fruit flies was composed of detecting probes and quality-control probes for C.

本研究选择线粒体DNA细胞色素氧化酶Ⅰ基因为分子标记基因,以双翅目实蝇科昆虫DNA序列为目标,建立了我国进境植物检疫害虫地中海实蝇Ceratitis capitata、芒果小条实蝇C.cosyra和纳塔尔小条实蝇C.rosa等生物芯片检测方法。

In order to develop an accurate and efficient method for the identification of tiger DNA,mtDNA Ctyb sequences of 5 tiger subspecies and 6 other feline species and 6 cervid species were downloaded from Genbank,and compared by Wdnasis(V2.5) software.

为了建立对虎DNA进行特异性检测的有效、精确的方法,本研究中,从Genbank下载虎及其他6种猫科动物和6种鹿科动物的mtDNA细胞色素b基因序列,并用Wdnasis(V2.5)软件进行了种间序列比较。

Different sizes AuNPs were obtained by adding different amount of trisodium citrate to reduce the chloroauric acid in a boiled and stirred condition, and then the protein was labeled by AuNPs.

用不同量的柠檬酸三钠在煮沸搅拌条件下还原氯金酸制得不同粒径的纳米金,利用纳米金易与蛋白结合的性质标记细胞色素P450。

Phylogenetic analysis and relative-rate test of 9 Clupeidae fishes were performed using thecytochromeb gene sequence data.

对鲱科9种鱼类的线粒体细胞色素b基因序列进行综合分析。

objective to study the effects of coadministration of aspirin with erythromycin ethylsuccinate on liver microsomal cytochrome p450 isoenzyme and multidrug resistance gene in rats.

目的以大鼠作为实验动物,形成疾病模型,研究阿司匹林合用琥乙红霉素对大鼠肝脏细胞色素p450同工酶和多药耐药基因的影响。

Because many antineoplastic drugs are also metabolized by the CYP system, coadministration with cART could result in either drug accumulation with increased toxicity, or decreased efficacy of one or both classes of drugs.

因为有许多抗肿瘤药物也是通过细胞色素酶系统来代谢的,当它们和抗逆转录病毒药物联合使用时会导致药物累积毒性增强,或者是某一类或几类药物疗效的下降。

The nucleotide composition and the phylogenetic relationship among 14 species in Coreidae based on 412bp sequences of Cyt b gene were studied. The results showed: the average contents of T, C, A and G were 34.2 %、11.4 %、35.7 % and 18.7 %, respectively; The content of A+T (69.9 %) was obviously higher than that of G+C (30.1 %), and come up to 82.8% at the third position of codon.

以线粒体细胞色素b基因作为分子标记,首次对缘蝽科4亚科14种昆虫进行序列测定,获得Cytb基因412bp的序列片段,该片段中碱基T、C、A、G的平均含量分别为34.2%、11.4%、35.7 %和18.7 %,A+T平均含量为69.9 %,明显高于G+C含量(30.1 %)。

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