细胞色素

Mitochondrial injury will ultimately lead to the release of cytochrome C into cytoplasm,as reflected by the two-fold increase of cytochrome C protein content in alcohol group.

线粒体损伤最终导致细胞色素C释放入胞浆，表现为模型组肝脏胞浆细胞色素C蛋白含量增加至正常对照组的2倍。

Objective To observe the pathological changes and investigate the hepatocyte apoptosis relating with CYP2E1 in alcoholic liver diseaseof rat. Method the Model of Alcoholic liver Disease: Rats were randomly divided into model group(n=37)and control group(n=33). The dose of 40％ethanol was administerd at 8g/kg body weight by garage twice daily for 8 weeks in model group, and control group was received isovolume saline by gavage. End of 8th week, the activities of serum ALT and AST were detect by autobiochemistry instrument. The pathological changes of liver was observed under light microscope after HE staining, hepatocyte apoptosis was detected by the TUNEL method.

目的： 研究酒精性肝病细胞凋亡程度与细胞色素P4502E1表达的关系方法：用酒精灌胃法制备大鼠酒精性肝病模型，将70只wistar大鼠随机分为正常对照组(33只)和酒精灌胃组(37只)，连续灌胃8周后病理切片观察细胞凋亡程度，干化学法测定ALT、AST的含量变化，用PCR法测定细胞色素P450 2E1的表达变化。

It was found from the structure-electrochemical studies of cytochrome c that (1) the secondary and tertiary structural changes of cytochrome c are greatly influenced by the reductions of tyrosine residues and iron ions in heme;(2) the reduction reaction dose not induce the configuration transition;(3)α helix, parallel β sheet, antiparallel β sheet and random coil take part in the complex secondary structural transitions;(4) mechanism and parameters about the electroreduction have also been obtained.

在细胞色素c的结构-电化学研究中发现：（1）细胞色素c的二级和三级结构受电场和酪氨酸残基及血红素中Fe的电化学还原的影响，其中酪氨酸残基还原的影响较大；（2）细胞色素c的电化学还原没有发生构型转化现象；（3）细胞色素c参与二级结构变化的构象有α螺旋，平行β折叠，反平行β折叠和无规卷曲；各组份随电位的变化复杂；（4）获得细胞色素c电化学还原的机理和参数

Results: When the concentrations of artemisinin were 1×10^(-4), 1×10^(-5) and 1×10^(-6)mol/L, the growth of cells was inhibited remarkably in a dose-dependent manner. Fluorescence staining showed obvious apoptosis, such as karyopyknosis and agglutination. RT-PCR assay shows the expression of Caspase-3. Artemisinin could lead to the decrease of mitochondrial cytochrome c concentration, which in turn lead to the increase of cytoplasmic cytochrome c concentration.

结果：青蒿素的浓度为1×10^(-4)，1×10^(-5)，1×10^(-6)mol/L时，细胞生长受到显著抑制，并呈剂量依赖性；Hoechst33342/PI双荧光染色可观察到明显的核浓缩、凝集等细胞凋亡表现；RT-PCR检测到Caspase-3的表达；Western-blot检测1×10^(-5)mol/L药物处理细胞后线粒体细胞色素C表达水平下调，细胞浆出现明显细胞色素C蛋白条带。

Moreover, CoI gene was more conservative and a lower evolutionary rate than Cyt b gene. CoI gene was an effective marker in analysing the phylogenesis of families in Passeriformes. It can be used in identifying the species of Passeriformes, but it was revealed that CoI gene was more suitable to identify the phylogenetic relationship of avian family unit than Cyt b gene, and CoI gene can be a molecular marker to identify avian species. But in species identification, CoI gene was less stable and accurate than Cyt b gene. We suggested youd better employ the other marker if you have the CoI gene only.(2) In the phylogenetic trees of birds from Lanius, L.

比较分析了雀形目6科15种鸟类的细胞色素b全序列和CoI基因部分序列，结果显示细胞色素b和CoI基因序列的变异位点分别为454个和366个、简约信息位点为337个和303个，而且线粒体CoI基因比细胞色素b基因略微保守、进化速率也较低；CoI基因在确定雀形目科级阶元之间的系统发生关系方面是一种有效的分子标记，同时它也能够用于雀形目鸟类的物种鉴定，但在物种鉴别方面不如细胞色素b基因稳定、准确。

IntroductionSmooth surfaced endoplasmic reticula contain plenty of cyto-chromes, which are mainly cytochrome P450 ( CYP450 ) and b5 and are very important in the process of the biotransformation of exogenetical fat - soluble compounds and the metabolism of some endogenetical matrices, such as bile acid forming^ bile pigment metabolism and steroid synthesis.

CYP450是50年代中期Williams和Klingeberg发现、60年代初木村和佐藤验证的一类b族细胞色素，由于还原型细胞色素与一氧化碳结合后在光谱的450nm处具有最高吸收峰，因而命名为CYP450以别与其它b族细胞色素，P是英文Peak的缩写。

Confocal microscope detection experiments showed that, in morulae and blastocysts, with theincreasing concentration of BPA, embryo of cytochrome C release rate increased. This showedthat the cytochrome C involved in BPA-induced the embryos apoptosis process. This processis regulated by the mitochondrial pathway.

激光共聚焦显微镜检测细胞色素C结果显示：在桑椹胚期和囊胚期，随着BPA处理浓度的升高，释放细胞色素C的胚胎比率逐渐增加，说明细胞色素C参与了BPA诱导胚胎细胞凋亡的过程，这一过程是通过线粒体通路来调节的。

Meanwhile, Va145 situating at the binding surface of the two proteins contributes greatly to the binding and electron transfer between Cyt 〓 and Cyt c.

而位于细胞色素〓与细胞色素c结合面上的Va145残基的改变对细胞色素〓与细胞色素c的结合与电子传递都有较大的影响。

Mitochondrial cytochrome b sequence were used to study the molecular evolution and phylogeny of Anser cygnoides(15 breed of Chinese goose), Anser anser(2 breed of domestic European goose) and Anser albifrons(the lesser white-fronted goose, Genbank accession number AF363031). Sequence analysis revealed that there were 29 variable sites and 4 haplotypes in 45 sequences, nucleotide diversity and haplotype diversity were 0.0068,0.45 respectively, insertion/deletion or frameshift mutation were not found in Cyt-b gene sequences(1143bp).

本实验测定了中国家鹅15个品种、欧洲鹅2个品种共44个个体的细胞色素b基因全序列，与Genbank库中自额雁细胞色素b基因序列合并在一起比对分析，17个家鹅品种和白额雁细胞色素b基因全序列均为1143bp，序列中没有缺失、插入或移码突变，共检测到29个变异位点、4种单倍型，核苷酸多样度和单倍型多样度分别为0.00648和0.45。

The effects of the mutation of some conserved residues on the structure, stability and redox potential of cytochrome 〓 have been discussed based on the structural comparison of the five mutants with wild type Tb5, respectively.

为阐明某些保守残基在细胞色素〓的结构和功能的相互关系以及电子传递过程中所起的作用，探讨制约或影响细胞色素〓的构效关系的主要因素，本论文对野生型Tb5及其一系列突变体进行了结构比较，并根据这些结果对细胞色素〓的三维结构与生物功能的相互关系进行了初步探讨。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。