细胞胚基

For these years, residua of metanephric blastema and delay of differentiation in the development of kidney have been considered the cause of nephroblastoma.

近年来的研究发现，肾发育过程中的胚基残留，分化障碍可能是肾母细胞瘤发病的原因之一。

Wilms" tumor is one of the most common solid tumors in children, and the genesis of Wilms" tumor is believed to be associated with delayed differentiation of metanephric blastema in fetus and retaining proliferation in postnatal period.

肾母细胞瘤（Wilms tumor， WT）是儿童最常见的实体瘤之一，其发病与后肾胚基细胞分化受阻并在出生后继续增殖有关。

The proembryo from zygotic cell divisions may be divided into three parts: proper, hypoblast and suspensor .

玉米合子细胞分裂形成的原胚分为胚柄、基与胚体三部分。

At the reproductive stage, RA68 was expressed in the inflorescence meristem, the tip of rachis branch, spikelet primordia, macrospore sac and pollen grains.

原位杂交分析结果表明：在幼苗期RA68主要在幼芽胚芽鞘的内外层细胞和幼叶原基的表层细胞中表达；转入生殖生长期后，在花序分生组织、枝梗原基顶端、花器官原基、大孢子囊和花粉粒中表达。

When the pollen tube comes into the micropyle and contact one of the synergids, both synergids do not show any sign of degeneration.

在二细胞原胚的顶细胞和基细胞中，均可观察到与合子中形态相似的双亲质体和线粒体。

It also indicates that the expression of WT1 is regulated strictly in time and space, and the aberrant expression of WT1 may result in retarded differentiation of blastemal cell leading NR or nephroblastoma.

提示在散发性肾母细胞瘤发生中除WT1突变外，WT1表达延迟可能是其中起重要作用的机制之一；WT1基因与肾母细胞瘤的组织分化有关，可能诱导胚基细胞向上皮细胞方向分化。

This result indicates that WT1 gene plays an important role in differentiation and development of fetal kidney and may be the factor that promotes metanephric blastemal cell to differentiate into epithelial cell.

结果显示小胎龄肾组织中WT1蛋白在胚基细胞和幼稚肾小球细胞核表达而大胎龄组肾组织中WT1在肾小管细胞胞浆表达，阳性率分别为57.1％(8/14)和46.2%(6/13)，提示WT1基因在胚胎肾分化发育的过程中起着重要作用，WT1蛋白可能是促进后肾胚基细胞向上皮细胞分化的调控因子，其表达在时间上和空间上都受到严格的调控，WT1的表达异常可能导致胚基细胞分化停滞。17例肾母细胞瘤WT1蛋白表达阳性率为41.2%(7/17)，阳性部位在胚基型和上皮型肿瘤细胞核，表达部位和阳性率与早期胚胎肾相似，其中间质型肾母细胞瘤均为阴性，胚基型和上皮型肾母细胞瘤阳性率70%(7/10)，两组间阳性率有显著差异。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示：未妊娠时，泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达，波形蛋白在子宫内膜基质细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，牦牛子宫任何部位均不表达角蛋白7；妊娠30天左右时，泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达，波形蛋白在子宫内膜基质细胞和内胚层细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，角蛋白7在尿囊细胞内表达，偶尔在腔上皮细胞的细胞核边缘表达；消化法进行原代培养时，组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞；分离得到的子宫内膜基质细胞活率达90%以上，并可在体外传代7次以上；分离得到的子宫内膜腺上皮细胞活率可达85%以上，并可在体外传代5次以上；RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长，维持子宫内膜基质细胞正常生长的FBS添加量为20%，维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

This experiment further investigated the possibility of differentiation of BMSCs in rats into neural stem cells in vitro, and into neural cells and neuroepithelial cells, so as to provide seed cells for cell transplantation of nervous system disease.

目的：已证实骨髓基质细胞可分化为中胚层组织细胞，实验予进一步探讨体外分离培养的骨髓基质细胞向神经干细胞分化的可能性，以及是否能继续定向分化为神经细胞及神经胶质细胞，为神经系统疾病细胞移植治疗提供种子细胞。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明，所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达；在缺乏AMBN情况下，与对照组相比，实验组牙胚在体外可以继续生长发育至钟状晚期，出现成釉细胞和成牙本质细胞的分化，成釉细胞可以分化成为分泌期型成釉细胞，胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体，缺乏溶酶体，表明对蛋白合成和脚的能力降低；实验组牙胚有牙尖形成和基质分泌，但牙尖形态异常，基质形成减少，牙尖周围基质最厚处为O.6卜m，明显薄于对照组的5.spin，基质中胶原纤维粗细不等，排列稀疏， 3 第四军医大学硕士学位论文未见钙化现象，充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程，影响胶原纤维和牙本质基质的合成，促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。