细胞结构

The book introduces the animal models representing natural infections ranging from uni- to multicellular and from endo- to ectoparasites in fish, chickens and mammals, including structural and physiological aspects of cell death; Role of apoptosis in non-mammalian host-parasite relationships; thionine-positive cells in relation to parasites; animal models for the study of innate immunity; interaction between sea lice and their hosts; pathophysiology of salmonids cryptobiosis and glossina-transmitted mammalian trypanosomiasis in livestock; avian coccidiosis; interactions betweem fish hosts and monogeneans.

该书介绍了鱼类，小鸡和哺乳动物从单细胞到多细胞和从内寄生虫到外寄生虫的天然传染的动物模型，包括细胞死亡的结构和生理学方向；非哺乳动物宿主和寄生虫关系中细胞凋亡的作用；硫堇阳性细胞与寄生虫的关系；用于先天免疫研究的动物模型；海虱与其宿主的相互作用；鲑鱼隐生想象的生理病理学和家畜中舌蝇传播的哺乳动物锥虫病；鸟类球虫病；鱼类宿主与单殖亚纲动物的相互作用。

The results indicate that:(1) Using SUZI method can improve the fertilization rates of denuded oocytessignificantly. This result suggests zona pellucida may be one of the reasons that makedenude oocytes matured in vitro have a clearly deficiency on fertilization in vitro.

结果表明：(1)利用精子带下注射技术可以极显著的提高去除卵丘细胞的卵母细胞的受精率，表明透明带结构的改变是去除卵丘细胞的卵母细胞存在受精障碍的重要原因之一。

Using cytohischemical staining methods, with the results of comparison the dynamics of proteins and polysaccharides in the anthe wall cells and colule cells of different developmental phases in female and male flowers, the anormogenesis of anther tapetum in the inflecting phase of from bisexual flower to unisexual flower was observed. In microspore developed phase, the tapetum functions of preserving and transportingmutritive material for microspore development and of secreting callosal enzyme for decompositing callosal cell lost no normal guadrant formed, of being abnormal meiose of pollen mother cells, and then, the stamen aborted selectively in female flowers and pistil in male flower.

利用细胞组织化学染色技术，对雌、雄花雄蕊花药壁细胞及花药内细胞发育过程中多糖及蛋白质动态进行了比较，实验中观察到雌花在从两性至单性花转变时期雄蕊绒毡层在整个发育过程中表现异常，在小孢子发育过程中未能起到贮藏、转运营养物质供小孢子发育及适时分泌胼胝质酶溶解胼胝质壁的功能，并且花粉母细胞减数分裂异常而未形成四分体结构，进而导致雌花雄蕊选择性败育，而雄花中雌蕊组织也发生了选择性败育过程。

What was observed under a light microscope included: tumor cells were mulberry and micropapillary shaped or were of glandule tubular arrangement; there was obvious interspace between cancer nest and neighboring areas; micropapillary was empty of fiber blood vessel axes, with micropapillae floating freely in spongy spaces and separated by fibrous septa.

光镜下特征性表现为肿瘤细胞呈桑椹状、微乳头状或小腺管样排列，癌巢与周围间质形成明显的空隙，微乳头缺乏纤维血管轴心，每个微乳头细胞团和周边的纤维组织均存在无细胞的间隙样结构；瘤细胞CerbB-2、CgA和EMA。

Increase the bone marrow cell component while reduce the fat component, abnormal the caryon and cytomembrane structure of bone marrow cell and stromal cell, enrich the ribose body, mitochondrion and rough surfaced endoplasmic reticulum in cytoplasm of several organs and tissues, embody abvious improvement of heart muscle, liver, immnune organs, incretory organs and sexual gland.

增加免疫介导型再障小鼠的骨髓细胞成分，减少脂肪成分。使骨髓细胞和基质细胞的细胞核和细胞膜结构趋于正常，使多种脏器组织的细胞浆内核糖体丰富，线粒体及粗面内质网发达。对心、肝、免疫器官、内分泌器官及性腺等亦有明显的改善作用。

The sex pheromone-producing gland of Ancylis.sativa Liu is amodified intersegmental membrane as a dorsal bag between eighth and ninthabdominal segments.

对枣粘虫雌蛾性信息素腺体的扫描和透射电镜观察表明，枣粘虫性信息素腺体是由第八、九腹节间的节间膜特化而成，是位于背部的一个囊状结构，它由两部分构成，前部的方形囊状体和后部的三角形囊状体，二者之间有一突起的脊；雌蛾静止时，腺体随第八和第九腹节一起嵌缩于第七腹节内，求偶时，腹部末端外伸，腺体细胞表皮外露，释放性信息素；腺体细胞呈方形，其表面覆盖几丁质表皮，表皮顶端无孔；细胞核很大，呈椭圆形，由双层核膜包被，位于细胞中下部，内有丰富的染色质；细胞质内有丰富的内质网，其内还有大量的大小不等的脂肪滴，以及线粒体和溶酶体等细胞器。

A strain of breast tumor cell line ZHL2006 was established and biologically identified. The ZHL2006 cells grew fast in vitro and the population doubling time was 43.6h. Electronmicroscopical observation showed that there were microvilli on the surface of the cells, nucleus irregularity, karyoplasmic ratio increased, nucleolus distinctness, et al.. Chromosome number and structure were abnormal. Immunohistochemistry showed that PCK were expressed. The ZHL2006 cell line could form onto clone in soft agar and had oncogenicity in athymic mice.

ZHL2006细胞在体外生长迅速，群体倍增时间为43.6h；倒置相差显微镜及Giemsa染色镜下观察细胞为多边形及梭形；透射电镜观察可见细胞表面有微绒毛，核高度不规则，核浆比例增大，核仁明显等；染色体数目和结构异常；广谱细胞角蛋白染色阳性，说明其自上皮而非来自基质；同时ZHL2006细胞系在软琼脂中可形成克隆，具裸鼠致瘤性。

There were nanobacteria in the nucleus and intracellar and extracellar endothelial cells under transmission electron microscopy after co-culture. The cell vacuolar degeneration, membrane structural necrosis, mitochondrin edema, mitochondrin vacuolar degeneration and lysosome myelinization were found in the endothelial cells.

混合培养后的内皮细胞透射电镜观察见细胞间、细胞内均有纳米细菌存在，细胞核内也可见纳米细菌，细胞空泡变，膜性结构类似坏死，部分线粒体水肿，空泡变，溶酶体呈现髓鞘图像。

In conclusion, features of morohopathology, IHC profile and ultrastructural findings reveal obvious divergence between CHP and HP and resemble MP, so we suggest that histogenesis of CHP is pericyte with myoid differentiation.

根据此研究之结果得知，无论在肿瘤发生部位、组织病理形态学、免疫组织化学染色结果以及超显微结构的特徵，CHP与HP具有明显的差异性，反而与MP之特徵较为相近，此结果可间接证实CHP之肿瘤细胞来源与MP的肿瘤细胞之来源较为相似，可能为血管周围细胞来源并具有类肌肉细胞的分化。

Methods BMSCs isolated from rabbit iliac bone marrow were culture-expanded in vitro. The injectable tissue-engineered bone constructed from autologous PRP, FG, and BMSCs was cultured in vitro, and its biological characteristics were observed including the time of gel formation, histological features, seed cell survival and microscopic structures. Results The constructed injectable tissue-engineered bone began gel formation within 20 to 30 s, and after a week-long culture, the gelatine began to degrade, and numerous well viable fusiform cells could be seen to adhere to the bottom of the Petil dish, Scanning electron microscopy identified globular and olivary cells embedded in the fibrin glue, and numerous small particles could be seen around of the cells.

从兔髂骨处抽取骨髓体外培养BMSCs并诱导向成骨细胞分化，抽取兔自体动脉血提取PRP，以FG、BMSCs、PRP共同构建可注射型组织下程骨并体外培养观察其生物学特性如凝胶形成时间，组织学特点、细胞存活情况及其超微结构特征等结果构建的可注射型组织工程骨可在短时间内形成凝胶，体外培养1周时其中细胞生长良好，电镜下见纤维蛋白网格结构致密，种子细胞及血小板颗粒分布广泛。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新