细胞结构

On the morphological differentiation,by inducing of fetal calf serum,it wasshown that the cytotrophoblast cells underwent aggregation first,and the adjacentcytoplasm membranes became tightly contacted through desomosomes andcytoskeleton.Then the adjacent cytoplasm membranes disintegrated fragmentallyto form fusion pore-like structure.Finally the multinuclear cells formed and inwhich the intercellular desmosomes disappeared.Immunocytochemical stainingfurther demonstrated that the formation of syncytium was through cell membranefusion rather than the amitosis process.

细胞形态分化的研究结果表明：早孕胎盘来源的细胞滋养层细胞在血清诱导下，首先发生聚集，并通过桥粒和细胞骨架使相邻的细胞膜紧密接触；之后细胞膜发生片段断裂，形成融合孔样结构；细胞间桥粒消失，最终形成多核的合体细胞；免疫细胞化学分析进一步证实合体化是通过细胞膜融合而非无丝分裂（amitosis即细胞核分裂而细胞质不分裂）实现的。

OBJECTIVE: Three sesquiterpene lactones 1-O-acetylbritannilactone, isoalantolactone and britannilactone were isolated from the roots of Inula helenium and the flowers of Inula japonica. The antitumor activities of the three sesquiterpenoids in the cell lines of HeLa, HEC-1, SHIN3, HOC-21 and HAC-2 were measured, and the relationship between structure and activity and the possible mechanisms were explored.

目的： 观察菊科植物中分离出的3种倍半萜化合物异土木香内酯、1-氧-乙酰大花旋覆花内酯和大花旋覆花内酯对体外培养的人子宫颈癌HeLa细胞、人子宫内膜癌HEC-1细胞、人卵巢透明细胞癌SHIN3及HOC-21细胞和人卵巢囊腺癌HAC-2细胞增殖的影响，研究这3种倍半萜化合物的体外抑制肿瘤细胞增殖作用及其结构与活性之间的构效关系，进而探讨3种倍半萜化合物的体外抑制肿瘤细胞增殖的可能机制。

We also have observed some organs pathological changes of juvenile Jian Carp, such as pancreas acinous gland and zymogen granules of acinous gland cell disappearing, intercellular substance hyperplasia and inflammatory cells soakage, hepatatrophia, liver cell granular or vacuolar degeneration and necrosis, karyolysis or pyknosis, glycogen granules decreasing, metanephros atrophy, metanephric canaliculus epithelium granular or vacuolar degeneration and necrosis, mitochondrion swelling and mitochondrion cristae disappearing, karyolysis, distal convolutal tubule microvilli desquamating, spleen marrow cell degeneration and necrosis, intercellular substance of spleen hyperplasia, spleen atresia, blood corpuscle disappearing.

后肾土黄色、淡褐色或苍白色，肾小管上皮细胞肿胀、颗粒或水泡变性、坏死，细胞内有大量血细胞流出，线粒体肿胀，嵴结构消失，细胞核溶解，肾间质甲状腺滤泡和拟淋巴细胞增生，远曲小管微绒毛脱落、管道细胞界限不清。心脏肌纤维肿胀、颗粒或空泡变性，严重的肌纤维溶解、变细或断裂，肌纤维间水肿、炎性细胞浸润，部分心肌细胞核浓缩。脾脏暗褐色，脾髓质细胞变性、坏死，拟淋巴细胞明显减少，淋巴细胞岛少见、岛中细胞成份减少，黑素巨噬细胞中心减少、体积缩小，脾脏网状基质水肿，脾窦闭锁，血细胞减少。

In order to investigate the theory and method in researching mechanical properties of hepatocellullar carcinoma cells with micropipette aspiration , hepatocellullar carcinoma cells.

细胞的力学特性与细胞的结构和功能密切相关，细胞粘弹性是细胞的一种本质属性，也是细胞力学特性研究的重要内容，微吸管法是一种在单个细胞水平上研究细胞力学特性的先进手段。

Elastic fibers were well-distributed,and vascular endothelial cell did not immigrate.We suggested that expression of ectogenic VEGF triggered paracrine and autocrine of VEGF of chondrocyte and co-acted with VEGF receptor 2 to enhance permeability of chondrocyte and improve internal construct of engineering cartilage,and prevent vascularize proceed.

转VEGF基因软骨细胞作为组织工程的种子细胞与pluronic F-127复合后可于裸鼠体内形成转基因组织工程软骨，与对照组相比，转VEGF基因组织工程软骨具良好的生物学特性，结构均一且与正常软骨组织相似，软骨ECM的GAG、COLⅡ、COLⅩ增多，RunX2、Sox9表达增高，细胞处于增生期的肥大状态，初步分析其原因可能是转染后外源性的VEGF持续表达触发了软骨细胞VEGF自分泌，并通过VEGFR-2作用于软骨细胞，提高了软骨细胞活性，促进其存活与增殖，但未在软骨组织内引起血管内皮细胞的迁移及小血管形成。

The effects of several components in the culture medium on Lithospermum erythrorhizon cell growth and secondary metabolite synthesis were studied, as well as structural dynamic model. The two-liquid-phase culture of Lithospermum erythrorhizon was carried out by choosing the proper organic solvent as the second phase. The bioactive carrier for adsorption was prepared and the condition of cell immobilization was determined. We combined the technique of two-liquid-phase culture and immobilization to carry out the culture. We chose the suitable type of reactor, studied its characteristics and results of cell culture using this reactor. The fed-batch operation was also studied on the basis of twoliquid-phase culture and immobilization used in culture in the reactor.

本文研究了紫草细胞悬浮培养中培养基中多种成分对细胞生长与次生代谢产物合成的影响，进行了结构化的动力学模型研究；通过选择合适的有机溶剂对紫草细胞进行了双液相培养研究；通过确定以吸附为细胞的固定化方法，进行了生物活性吸附载体的制备与固定化细胞的制备研究；并结合双液相培养技术，对紫草细胞进行了固定化培养及其动力学模型的研究；对反应器进行选型，并进行冷模与热模研究；在反应器中进行了固定化紫草细胞的双液相培养条件下的流加操作研究。

That GER cells which are likely hair cell progenitors could be cultured in vitro and generate stereociliary bundle-like structure when forced to express Hath1 suggests that the misexpression of Hath1 probably can induce the predifferentiation of pure hair cell progenitors into hair cells in vitro.

GER细胞作为毛细胞前体细胞可以实现体外原代培养，在Hath1基因重表达情况下，部分细胞的表面可见不成熟的纤毛样结构，提示Hath1基因也许可以诱导离体培养下的纯的毛细胞前体细胞向毛细胞的初步分化。

When Fas and c-myc protein were coexpressed in cytoplasm, a strong positive sign appeared in highly differentiated osteosarcoma, fibrous dysplasia of bone and osteoplast of bone callus, a weak sign was found in low-differentiated tumour cells and fibroblasts.

Fas和c-myc蛋白胞浆内阳性，在骨肉瘤的高分化细胞(骨母细胞型和软骨细胞型瘤细胞)和骨化性纤维瘤、骨纤维结构不良、骨痂组织中骨母细胞呈强表达；而在低分化瘤细胞和纤维母细胞则呈弱表达。

Affect on the liver pathological state after XIEZHIPING treated: the golden hamster hepatic tissue morphous structure of normal control was normal, which had regular hepatic lobules and no lipid droplet;that of model control manifested diffuse heavy adipose degeneration and heavy ballooning degeneration with inflammatory cell infiltrate which mainly composed of lympholeukocyte;that of high dose group showed midrange ballooning degeneration with chronic inflammatory cell infiltrate which mainly composed of lympholeukocyte and plasmocyte;that of media dose group indicated slight focus ballooning degeneration with a small quantity normal hepatic cells in some locals;that of low dose group appeared midrange adipose degeneration with a few lympholeukocyte cell infiltrate in the parts of central veins and port area.

泻脂平治疗后对肝脏病理状况的影响：空白对照组金黄地鼠的肝组织形态结构正常，肝小叶规则，无脂滴发现；模型对照组肝脏弥漫性重度脂肪变性，发生重度气球样变，伴见以淋巴细胞为主的慢性炎性细胞浸润；泻脂平高剂量组肝细胞呈现中度气球样变，伴见以淋巴细胞、浆细胞为主的慢性炎性细胞浸润；中剂量组肝细胞轻度灶性气球样变，局部可见少量正常肝细胞；低剂量组肝细胞呈中度脂肪变性，可见中央静脉及汇管区些许淋巴细胞浸润。

Before treatment,there are abundant microvtlli on the cell surface,many filoplodia on the cell rim,few of endoplasmic reticulums , Golgi complexes and mitochondrions with abnormal structure and lots of free ribosomes in the cytoplasm. The shape of nucleus is irregular with high nuclear-cytoplasmic ratio, many dark pellets of heteromatin and a few of nucleoluses in which there ar...

经1.5×10~2g/L地塞米松处理后，细胞表面微绒毛和丝状伪足显著减少或消失，出现皱褶状和小泡状结构；细胞核形规则，核质比值减小，异染色质团块减少，核仁数目减少、结构致密；细胞质中粗糙型内质网、高尔基体、线粒体数量增多，形态结构典型，游离核糖体减少，这些变化说明地塞米松改变了MGc80-3细胞的超微结构特征，具有明显的诱导分化作用。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新