细胞组织学诊断

Abstract］ objective to study the pathological features and histopathological type and differential diagnosis of hepatic focal nodular hyperplasia.methods the clinicopathological characteristics of 40 cases of fnh were studied.all were evaluted by use of paraffin embedded sections and he staining before light microcope observation.results there were 28 females and 12 males fnh patients whose age were from 16 to 62 years（median 41.3）,all alpha-fetoprotein was negative and had no hepatitis history.25 cases were classic type showed characteristic central stellate fibrotic scar,composed of fibrous connestive tissue and tortuous blood vessels.8 cases were telangiectic type,the left were mixed type and adenomatoid type.conclusion fnh is a reactive proliferation of hepatic cells to local blood vessel anomalies,it is not realy a tumor.its differential diagnosis includes hepatic adenomatous hyperplasia nodule,hepatic anaplasia nodular hyperplasia,fibrolamellar hepatocellular carcinoma and hepatocellular adenoma.

目的 探讨肝局灶性结节性增生的病理形态特点、组织分型及鉴别诊断。方法分析40例肝局灶性结节性增生的临床资料，并采用石蜡包埋he染色光镜下观察其组织学特点。结果 40例肝局灶性结节性增生患者中，女28例，男12例，年龄18~62岁，平均年龄41.3岁，所有病例术前均无肝炎病史，甲胎蛋白阴性，组织学上25例为经典型，有特征性的中央纤维瘢痕，由纤维结缔组织及扭曲血管组成。8例为毛细血管扩张型，其余为混合型及腺瘤样增生型。结论肝结节性增生是一种肝细胞对局部血管的异常反应性增生，并非真性肿瘤，主要与肝腺瘤样增生性结节、肝间变性结节状增生、肝纤维板层癌及肝细胞腺瘤鉴别。

Results: 4 cases were geminoma, 3 cases were teratoma, 1 case was choriocarcinoma, 4 cases were glioma, 2 cases were meningiomas, pineoblstoma, neuroblastoma, dermoid cyst and arachnoid cyst each had one case. It maybe very difficult if we diagnosed only through MR signals. Due to the multitude origin of histology, we can diagnose through some special signals, such as adipose, cystic degeneration, hemorrhage and implant dissemination. We can also combined with clinical examination which is help to differential diagnosis.

结果：生殖细胞瘤4例，畸胎瘤3例，1例绒毛膜癌，胶质瘤4例，脑膜瘤2例，松果体母细胞瘤、神经母细胞瘤、皮样囊肿及蛛网膜囊肿各1例，仅通过MR信号较难诊断，由于肿瘤组织学来源多，可通过一些特殊信号如脂肪、囊变、出血、种植播散等，并结合临床检查来进行鉴别诊断。

As a diagnostic aid to traditional histologic evaluation, this assay can have significant clinical impact and improve classification of melanocytic neoplasms with conflicting morphologic criteria.

FISH检测是对传统组织学诊断的辅助，具有重要的临床意义，能提高那些与形态诊断标准有冲突的黑色素细胞肿瘤的分类。

Sample collection: Two hundred and fifteen sera samples of normal population were collected from the peripheral blood of healthy blood donors of Shenyang, and the sera were separated by centrifugation after blood clotting; The patients sera were collected from the outpatients and inpatients of No. 1 hospital of China Medical University and the Second hospital of Jilin University. Syphilis was confirmed by clinical and serum test of TPHA and RPR; That of pemphigus vulgarism was confirmed not only by clinical, histopathological examination, but also by direct and indirect immunofluorescence test. DIF test on lesion or non-lesion skin showed immunoglobin and/or C3 deposits in the intercellular space of epidermis, and IIF test showed positive anti- intercellular antibody in patient serum using mouse's or rat's tongue or esophagus as substrate.

标本的收集：正常人的血清：采集沈阳市正常献血员外周静脉血共215份，待血凝后离心分离血清；患者的血清：来自中国医科大学一院及吉林大学二院门诊及住院部的病人，梅毒患者除了病史及临床表现外，全部经血清学TPHA及RPR确认；寻常性天疱疮患者除了临床及组织病理诊断外，还进行了直间接免疫荧光的检测，直接免诊断疫荧光：在皮损区及非皮损区的表皮细胞间显示IgG和/或C3补体沉积；间接免疫荧光用鼠或兔的舌或食管作为底物，在病人的血清中显示有肯定的细胞间抗体。

Totally 31 patients with bacteriologically or histologically confirmed extrapulmonary tuberculosis in Peking Union Medical College Hospital received T-SPOT.TB assay to detect early secreting antigen target 6 or culture filtrate protein 10 peptides-specific T cells in the peripheral blood mononuclear cells.

对北京协和医院根据细菌学或／和组织学诊断的肺外结核患者31例，应用酶联免疫斑点技术检测6kD早期分泌靶向抗原和10kD培养滤过蛋白肽段库刺激后外周血中释放γ-干扰素的特异性T细胞。

Objective To investigate clinicopathological characteristics of unicystic ameloblastoma and histological markers for differential diagnosis with odontogenic cyst.

目的 研究单囊型成釉细胞瘤的临床病理及凝集素免疫组织化学特点，探索有助于诊断和鉴别诊断的组织学标记物。

During the surgery, the lesion was found to be a solid tumor. A surgical wedge biopsy was done and purulent dark yellow fluid was drained from the center of the mass. Histologically, there was a solid pattern of rhabdoid and anaplastic round or oval cells haphazardly arranged with poor differentiation. Immunohistochemical staining was positive for vimentin, a mesenchymal marker; the reticulin staining was consistent with carcinoma.

我们实施了手术切片及肿瘤中心脓状物引流，病理组织学上，我们发现了不规则或未分化的横纹状、圆形及椭圆形细胞；免疫组织染色上，细胞广泛的对非上皮标记vimentin呈阳性反应、且其reticulin染色呈现出腺癌的形式；肉瘤及腺癌的特色皆可在切片中看到，所以我们的诊断是类肉瘤性线癌。

These results were compared with the cytohistologic diagnosis according to the final clinical general diagnosis as standard for pancreatic cancer and mucinous neoplasms to evaluate the potential value of the mucins expression pattern in diagnosis of pancreatic cancer and mucinous neoplasms.

用免疫组化S-P方法检测粘蛋白家族(MUC1、MUC2、MUC5AC)在54例胰腺占位性病变的EUS-FNA组织标本的表达情况，根据临床综合判断的诊断，与细胞组织学检查结果比较。

Acetic acid stain was used in abnormal patients. visual observation, photography, colposcopy and cervical biopsy were used. tbs （2001） report grading system was used in cytological diagnosis including the positive diagnosis of atypical squamous cells or more specimens.

细胞学诊断采用tbs（2001）分级报告系统，阳性诊断包括意义不明的不典型鳞状细胞及以上标本；且所有ascus以上病变的患者全部有病理组织学诊断结果，并对两者进行了对照分析。

Methods Thin Prep liquid-based cytological examination was used in 17520 patients. 5% acetic acid stain was used in abnormal patients. Visual observation, photography, colposcopy and cervical biopsy were used. TBS （2001） report grading system was used in cytological diagnosis including the positive diagnosis of atypical squamous cells or more specimens.

细胞学诊断采用TBS（2001）分级报告系统，阳性诊断包括意义不明的不典型鳞状细胞及以上标本；且所有ASCUS以上病变的患者全部有病理组织学诊断结果，并对两者进行了对照分析。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失