细胞的

On basis of mention , we investigate the ability of isolation, purification and expansion of adult rat mesenchymal stem cells to nerve cells in vitro.MethodsBone marrow mononuclear cells from adult rat femur and tibia were obtained sterilely by Percoll ( 1. 073 103 g/L ) gradient centrifugation .

根据MSCs与其它细胞的密度不同而采用Percoll(1.073×10~3g／L)分离液将其分离，此方法分离MSCs所获得的细胞种类较单一，Percoll分离液将骨髓细胞分为三层，取含MSCs的低密度层进行培养。

We tested whether inhibitory GABAergic synaptic transmission regulates Xenopus optic tectal cell dendritic arbor development in vivo by expressing a peptide corresponding to an intracellular loop ofthe gamma2 subunit of type A GABA receptors GABA(AR, which is required to anchor GABA receptors to the postsynaptic scaffold.

我们想证明如下设想：抑制性GABA能突触传递是否能调节早蟾视顶盖细胞的树突发育。因为GABA受体的gamma2亚基有一段细胞内的跨膜端序列，这段序列对锚定GABAA受体到突触后膜是必须的，所以我们的主要方法是设计了一段特异性的争对细胞内段的序列，这样就特异性的阻断了单个细胞GABA能的突触传递。

Methods: The human osteoblasts were isolated from the trabecular bone by ...

结果：所分离培养的细胞具有成骨细胞的形态特征，保持了其在体内的功能；MG63细胞株和人成骨细胞均表达PRA和PRBmRNA和蛋白质。

Methods: the expression of hALK in HepG2 cells was observed immunocytochemistry and reverse transcriptive PCR. Proliferation of HepG2 cells after neutralization was detected by 3H-TdR incorporation approach.

用免疫细胞化学以及RT-PCR的方法检测HepG2细胞hALR蛋白及mRNA的表达；用3H-TdR掺入法检测hALR特异性单克隆抗体中和hALR后HepG2细胞的增殖情况。

Analysis of the cells in HCC supports the presence of cells with stem-cell properties (e.g., immortality, transplantability, and resistance to therapy).

对于肝癌细胞的研究我们知道肝细胞型肝癌中的细胞具有干细胞特性，如永生性，可移植性以及对治疗手段的抵抗性。

They can reach 72.04% and 84.5% separately(P.05). 2. The methods such as Percoll uncontinuous density gradient centrifugation, isolation according to the different adhesiveness can be used to isolate and purify rabbit SSC effectively. Spermatogonia mainly distribute between the gradient 27% to 35%(density between 1.0410g/ml to 1.0508g/ml).

Percoll 不连续密度梯度离心和差异粘附等方法可以有效的对兔精原细胞进行分离纯化，其中精原细胞主要分布于密度梯度为27%-35%(密度 1.0410g/ml~1.0508g/ml)的带间，此带中精原细胞的纯度平均达到 60.42%(P.01)。

Results Human complement can be activated by unsensitized EL-4 cell through the first pathway.

结果 未致敏EL-4细胞可直接以Ca2+、Mg2+离子依赖的第一途径活化人血清补体系统，在细胞数为1×104～2×104时，1：200稀释的人血清补体为EL-4细胞的非致死剂量。

The results showed that though testosterone or 17-β-estradiol did not affect the expression of these AM in unstimulated HUVEC, both of them transiently increased the expression of E-selectin and VCAM-1 in TNF-α-stimulated HUVEC.

近几年研究发现，粥样硬化与炎症及免疫反应有关，TNF-α等细胞因子可以通过刺激血管内皮细胞粘附分子的表达及血管平滑肌细胞的增殖等而在粥样硬化的形成中起重要作用。

Results After six passages in BHK-21 cells, cytopathic effect was observed by microscopy. The rescued virus was rejuvenated once in unweaned mice, and then came back to cell passage. We found that the poly tract of rescued virus was shortened when the virus was passaged twice in BHK-21 cells. Although the data of LD50 in mice and TCID50 in BHK-21 cells showed that the virulence and infectivity of genetic engineering viruses were lower than its parental virus, no significant difference was observed between the genetic engineering viruses with the different length poly tract.

我们发现，基因工程毒在BHK-21上连续传代至第6代时，可见明显的致细胞病变效应(cytopathic effect，CPE)；在经过一代乳鼠接种之后再重新适应到BHK-21细胞的过程中，poly区段出现了缩短现象；乳鼠致病性试验和BHK-21细胞TCID50测定结果表明，尽管基因工程毒与母本毒相比毒力偏低，但含有不同长度poly的基因工程毒之间并无显著差异。

Methods: The bacteria inhibiting experiment in vitro was used to observe the bacteria inhibiting effects of usnic acid material and SMEDDS. The cytotoxic action on peritoneal macrophage and the mouse peritoneal macrophage releasing TNF-α induced by thermal inactivation bacillus coli of usnic acid material and SMEDDS were determined by MTT.

采用体外抑菌实验观察松萝酸原料及自微乳的抑菌效果；采用MTT法考察松萝酸原料及自微乳制剂对腹腔巨噬细胞的细胞毒作用，及对热灭活大肠杆菌诱导小鼠腹腔巨噬细胞释放TNF-α的影响。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。