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The results showed that the cells had classic characteristics of osteoclasts: multinucleated giant cells, staining positively for TRAP in cells, forming bone absorptive lacunae on the bone slices.

结果表明,直接分离的细胞具有典型的破骨细胞的形态特点:属多核大细胞,TRAP染色阳性,能在骨片表面形成吸收陷窝。

Specific siRNA was used to target LMO2 in Molt-4 cells. Results Higher expression of LMO2 was detected in some of patients with acute myeloblastic leukemia or acute lymphoblastic leukemia. However, enforced expression of LMO2 in K562 cells decreased cellular clonogenecity, suggesting the activation of the gene may not be important in the pathogenesis of myeloid leukemia.

结果 LMO2基因在很多急性髓系和淋巴系白血病患者的表达水平高于慢性白血病患者及正常骨髓细胞,但LMO2转染的K562细胞克隆形成率下降,提示该基因在髓系白血病细胞的表达异常是一种伴随结果而不具有致癌作用。

The results showed that the rates of mature oocyte were 75.2%, 73.1%, 69.8%, 63.5%and oocyte at telophase of MI were 16.3%, 15.9%, 16.9%, 27.0%, respectively. The rates of maturation of oocytes cultured in TCM199 with serum, EGF and TGFαwere significantly higher than that of with BSA team (P<0.05) and the number of oocyte stayed at telophase of MI in TCM199 with serum, EGF and TGFαteam were significantly less than that with BSA team(P<0.05); Significant higher rates (66.6%, 66.6%, 73.6%) of normalα-tubulin distribution in oocytes cultured in TCM199+ serum, EGF and TGFαwere compared to that of TCM199+BSA(43.3%)(P<0.05). The rates of oocyte with cortical granules in cortex were 58.8%, 33.9%, 54.7%and 47.9%respectively, there was significant difference between oocytes cultured with serum, EGF and BSA(P<0.05). In conclusion, TGFαand EGF can promote the oocyte nuclear transition from telophaseⅠto metaphase of meiosisⅡ, and improved the expression and distribution ofα-tubulin during the ovine oocytes maturation; EGF and serum could promote oocyte cytoplasm maturation. The results suggested that EGF and TGFαmight substitute some substance in serum to improve the quality of oocyte nucleus maturation in vitro, but EGF might be more functional than TGFαto promote the maturation of ovine oocyte ooplasm.

三、EGF和TGFα对卵母细胞成熟的影响未成熟卵母细胞分别在TCM199基础培养液+血清、TCM199基础培养液+BSA+EGF、TCM199基础培养液+BSA+TGFα和TCM199基础培养液+BSA四组成熟培养系统中成熟培养,22小时后上述各组卵母细胞的核成熟率分别为75.2%、73.1%、69.8%、63.5%,处于第一次减数分裂末期的比率分别为16.3%、15.9%、16.9%、27.0%,在添加血清、EGF、TGFα各组中核成熟率显著高于其它各组(P<0.05),处于末期的比例明显低于其它各组(P<0.05):添加血清、EGF、TGFα各组在a-Tubulin蛋白的分布与表达上(66.6%、66.6%、73.6%)明显高于BSA组的正常率43.3%(P<0.05);各组CG发生迁移较好的卵母细胞的比率分别为58.8%、33.9%、54.7%、47.9%,血清组和添加EGF组CG迁移至皮质区的比例明显高于仅添加BSA处理组(P<0.05)。

To determine whether F3, similar to other large DNA iruses, encoded proteins that lock or delay apoptosis, mock- and F3-infected FHM cells were osmotically shocked and assayed for DNA fragmentation 3 hours later. NA fragmentation was clearly seen whether or not shocked cells were preiously infected with F3, indicating that infection with F3 did not block apoptosis induced by osmotic shock in FHM cells.

为了证明F3是否象其大DNA病毒一样编码能阻止或延迟凋亡的蛋白,在模拟感染和正常感染的FHM细胞被渗透压休克后3小时检测DNA片段化;结果显示无论是否休克F3感染都能诱导FHM细胞的DNA片段化,渗透压休克并不能阻止细胞凋亡。

BMP, TGF-β and bFGF possessed their own different characteristics of expression and distribution and cooperatively modulated proliferation of osteogenetic cells and differentiation of chondrocytes and osteoblasts for fracture healing.

BMP、TGF-β和bFGF有着各自的表达和分布特点,并共同调解骨原细胞的增殖和成骨细胞、软骨细胞的分化,最终完成骨折修复。

Objective To investigate the proliferative and osteogenic activity of periosteal osteoblasts, calvarial osteoblasts and bone marrow stromal cells from the same embryo.

目的 比较从同一胚胎来源的骨膜成骨细胞、颅骨成骨细胞和骨髓基质干细胞的分裂增殖能力和细胞的功能状态。

The mechanism of its whitening function was studied at cell level,and we found that there are three approaches by which GN can exert its function:① inhibiting the activity of tyrosinase;② repressing the growth of melanocyte;③ stimulating skin cell to produce lysosome to phagocytose the melanin.

一种海洋生物提取物GN具有美白的功效,从细胞水平探讨其作用机理,发现GN不是通过一种途径,而是通过多种途径起作用:①抑制酪氨酸酶的活性;②可能阻碍黑素细胞的生长;③可能刺激细胞产生溶酶体吞噬黑色

In situ hybridization and immunocytochemical studies indicated that treatment with 17beta -estradiol increased the number of aromatase-immunoreactive cells and their immunoreactivities, especially in the preoptic area of the hypothalamus, where the density of the aromatase-immunoreactive cells was 2.6 folds higher than the control group. Results indicated that brain aromatase may be involved in the feminization induced by 17beta -estradiol of the grey mullet.

原位杂交和免疫细胞化学定位表明:雌二醇处理组鲻鱼脑内芳香化酶阳性细胞数量和反应强度均提高,尤其在下丘脑视前区,芳香化酶阳性细胞的分布密度为对照组的2.6倍,说明17beta-雌二醇诱导幼鲻雌性化可能与脑芳香化酶的表达有关。

These findings have suggested the development stages at which to transplant cells--for instance, photoreceptor cells need to be relatively more mature than stem cells, according to Thomas Reh, who studies retinal development at the University of Washington.

在华盛顿大学从事视网膜发育研究的Thomas Reh表示,这些发现提示,进行细胞移植--比如感光细胞的移植时,被移植细胞的发育阶段应比干细胞要相对成熟一些。

RESULTS: Similar to the control, the cells in Jagged1 group displayed irregular shape with spiny or sheetlike dendrites and the cellular surface was rough. In contrast, the cells in DAPT group exhibited little dendritic processes and most of them were of round shape and with smooth surface.

结果: 与对照组相似, Jagged1组的细胞形态不规则,伪足分化和突出的片状结构较为明显,且表面粗糙;相反, DAPT组细胞的伪足不明显,多数细胞趋于圆形,表面相对光滑。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。