细胞的

Methods: Density gradient centrifugalization and absorption technique wererespectively applied to detach peripheral blood mononuclear cells andperipheral blood lymphocytes,T lymphocytes were got by Nylon postabsorption method, and CD4+T and CD8+T lymphocytes divided with anti- CD4 andanti-CD8 antibody by alexin cytotoxic method.

密度梯度离心法分离外周血单个核细胞，玻璃器皿吸附法分离外周血淋巴细胞，尼龙棉柱法分离 T 淋巴细胞，补体介导的细胞毒法分别制备 CD4+T和 CD8+T 淋巴细胞，采用 T 淋巴细胞与角质形成细胞共培养技术研究银屑病患者外周血 T 细胞对角质形成细胞的影响，免疫组化法检测共培养角质形成细胞 Ki67、c-Myc 及 Bcl-xL 蛋白的表达。

The two neck cells of the archegonium divided to fo rm four neck cells before fertilization, this division happened prior to the div ision of the central cell, but at times they divided synchronously.

颈卵器的2个颈细胞在受精前进行一次斜向分裂形成4个颈细胞，此分裂一般早于中央细胞的分裂，偶尔也可见颈细胞和中央细胞同时分裂。

Methods the rabbit arterial smooth muscle cells were cultured ex vivo, cells of 4-7 generation were divided into groups interfered with ang-（1-7）, positive control groupsinterfered with ang-ii(10-6mol/l) and blank control group .cell counting with trypanblau dying and mtt were used to evaluate the growth of cells in different groups and results were compared .

体外培养兔肺动脉平滑肌细胞，鉴定后传代培养，取4～7代细胞，应用不同浓度ang-（1-7）干预，并与血管紧张素-ii(ang-ii，浓度10-6mol/l)、空白组对照。通过台盼蓝染色法细胞计数、四甲基偶氮唑蓝比色法，确定肺动脉平滑肌细胞的生长趋势变化。

Quantitative chromatics analysis of large bowel tumorous adenoma and adenocarci-noma was made by the methods of computer image processing.

本文通过对大肠腺瘤和腺癌组织进行计算机图像定量分析，表明大肠腺瘤和腺癌的腺上皮细胞以正常杯状细胞和柱状细胞的色度学特征分布为基准可划分为两大类：杯状细胞性腺上皮和柱状细胞性腺上皮。

Cell cycle is the cell through a series of orderly changes in the cells to achieve cell growth and proliferation process of cell cycle regulation and individual development, differentiation, growth, regeneration, aging and cancer cells is closely related to eukaryotic cell cycle mitosis two key processes, namely DNA replication and sister chromatid evenly assigned to the sub-cells.

细胞周期是指细胞通过一系列有序的细胞内变化，实现细胞生长和分裂增殖的过程细胞周期的调控与个体发育、分化、生长、再生、衰老以及细胞的癌变密切相关真核细胞有丝分裂周期有两个关键的过程，即DNA的复制和姐妹染色体均衡地分配到子细胞中。

Manual microdissection in the isolation and purification of ciliated cells, can reduce the interference of impure cells, increase the cell activity, and is a satisfied technique to offer pure cells.

将手工显微分离技术应用于纤毛细胞分离和纯化降低了杂质细胞干扰，提高了细胞活性，满足了提供纯净目的细胞的实验要求。

Methods:Using cell culture technique,the inhibitory effects of NCTD on 3AO and AO cells were measured by way of crystal violet dyeing.The alterations of cell cycle induced by NCTD were analyzed with flow cytometry.

利用细胞培养技术，以结晶紫染色法测定NCTD对卵巢癌3AO和AO细胞的抑制作用，并利用流式细胞仪测定分析NCTD作用下两种肿瘤细胞的细胞周期变化。

The animal experiment stage: 40 rabbits underwent corneal epithelium curettement and limbal ablation to induce limbal stem cell deficiency and were randomly divided into four groups, simple amniotic membrane group, non-frozen cells group, glycerol group and dimethyl sulfoxide group(n=10 for each group). Searching for the activity of frozen and non-frozen cells more completely by transplanting subculturing cells and observing the accomplishment of cells function.

动物实验阶段：将40只角膜缘干细胞损伤的模型兔随机分成单纯羊膜组、未冷冻细胞组、DMSO保存细胞组和甘油保存细胞组，每组10只，用传代培养2周后的角膜缘上皮细胞作移植实验，观察细胞体外功能完成情况来进一步比较深低温保存前后角膜缘上皮细胞的活性。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分为4个部分，第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究，确立了一种较好的培养方法：与颗粒细胞共培养，找到了一种适合猪卵母细胞体外成熟的培养基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸，成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%，国外报道的最高成熟率为(85.7±4.1)%；第二部分对猪卵母细胞孤雌激活的化学方法进行了研究，确立了化学激活猪卵母细胞的两种最佳方法：1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min，再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中，卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min，再与8mM的DTT共孵育30min，卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%；第三部分对孤雌激活胚胎的培养条件进行了研究，确立了一种最佳的胚胎培养条件：在SOF简单培养基中添加颗粒细胞进行前3天的培养，然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养，其桑椹胚和囊胚的发育率为(59.5±3.2)%；第四部分研究了IGF-I

In Chapter Two, some methods such as electron microscope, DNA ending label and MTT measurement were used to studied the changes of apoptosis, necroses and cell vigour of leukaemia K562 cell trunk and HL-60 cell trunk irradiated with millimeter wave. Different effects of millimeter wave to leukaemia cell trunks and human marrow cells were observed clearly. Human marrow cells were not so sensitive to millimeter wave radiation as leukaemia cells.

第二章，利用电镜技术、DNA末端标记、MTT活力测定等方法，研究了毫米波对红白血病K562细胞株、人类急性早幼粒白血病细胞株HL-60及正常人骨髓单核细胞的细胞超微结构、细胞凋亡、细胞活力的影响，结果发现毫米波辐射对正常人骨髓细胞及白血病细胞的影响明显不同，正常人骨髓细胞所受影响较小，特别是功率密度小的毫米波对其无影响；而白血病细胞受辐射后发生显著的凋亡或坏死，细胞活力显著下降，该效应具有明显的功率窗和时间窗。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。