细胞的

Allabove indicated that the more antitumor ability of PELs was not theirmount,perhaps,but their quality.

以上这些说明微环境中一定水平IL-2的存在能使肿瘤的细胞免疫应答速度加快，免疫细胞获得杀伤能力所需时间短并伤百分率高，从而使在这种微环境中肿瘤细胞的生长明显受到抑制，同时也说明mIL-2〓诱导的PEL抗肿瘤能力，并非是通过免疫细胞数量上的增加来实现。

1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异；采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异；采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

This coupling of cell division and cell survival is unique – other genes that promote cell division, for example, Myc, also sensitize a cell to death.

这种促进细胞分裂和细胞生存的作用的偶联是唯一的——其它能促进细胞分裂的基因如Myc，却会促进细胞的死亡。

The inward current in OHCs was totally inhibited after the superfusion of 100μmolIL and 200μmo1/L linopirdine respectively. The outward rectifier K(superscript +) current I(subscript K was the dominant K(superscript +) current in the whole cell currents in Deiters' cells. In the presence of 200μmol/L linopirdine, the current was not significantly affected.

在细胞外液中，加入100μmol/L利诺吡啶后，OHCs中的四乙基二乙胺敏感的钾电流峰电流成分被抑制，稳态电流幅值减小，且电流的失活时间常数明显延长；在细胞外液中加入100μmol/L利诺吡吮后，OHCs的内向性钾电流I被完全抑制；而细胞外液中利诺吡啶终浓度为200μmol/L时，Deiters 细胞的外向整流性钾电流幅值无明显变化。

We review the effect of rare earth ions on bone metabolism as the following three aspects:(1)effect of rare earth ions on differentiation and function expression of osteoblasts and osteoclasts;(2)effect of rare earth ions on the osteogenic and adipogenic differentiation of bone marrow stromal cells;(3)effect of rare earth ions on bone mineral phase of animals.

本文回顾了稀土离子对骨代谢影响的研究进展，探讨了其药用和安全性问题，主要从以下三个方面进行综述：(1)稀土离子对成骨细胞细胞和破骨细胞的分化和功能表达的影响；(2)稀土离子对骨髓基质细胞成骨分化和成脂分化的影响；(3)稀土离子对动物骨矿物相的影响。

Results: T cell anergy was antigen-specific. The state of T cell anergy can be reversed by PHA, CD3 mAb and PMA plus A23187. IL-2 can prevent the induction of T cell anergy, but it can not reverse the state of un-responsiveness. IL-2 and IFN mRNA can not express in anergic T cells. In contrast, IL-4 and IL-10 mRNA were detectable.

结果：T细胞无能为抗原特异性的，多克隆激活剂PHA、CD3单抗和PMA+A23187可以恢复无能T细胞的活性；IL-2可以阻止T细胞无能的形成，但不能逆转其耐受状态；无能T细胞IL2和IFN mRNA是关闭的，而IL-4和IL-10 mRNA则是开放的。

Methods Ovarian GC DNA fragments of both atretic and developing follicles before and after estrogen (1 μg/ml), androgen (1 μg/ml) treatments were analysed by agarose gel electrophoresis. The expression of bcl-2 mRNA was also determined by northern blotting in GC of developing follicle after sex steroids addition. Results Internucleosomal DNA cleavage occurred in granulosa cells in atretic follicles and after androgen treatment.

应用细胞培养、选择性DNA抽提和琼脂糖凝胶电泳技术分析闭锁卵泡和发育中卵泡颗粒细胞的凋亡状况，及雌激素(1 μg/ml)、雄激素(1 μg/ml)对体外培养发育中的卵泡颗粒细胞凋亡的作用；用Northern印迹杂交技术检测经雌、雄激素刺激后发育中的卵泡颗粒细胞bcl-2基因mRNA表达的变化。

The illumination can urge the human body cell 5 times of speeds to grow,when the cytochrome in the cytogenous gland is started by the lamp-house,promotes glucose transformate to ATP,the cell metabolism energy rise, thus stimulates the collogen hyperplasia,the different lamp-house can have the different influence to the skin.

光照能促使人体细胞5倍速生长，当细胞粒腺体内的细胞色素被光源启动，促进葡萄糖转化为ATP，细胞的新陈代谢能量上升，从而刺激胶原蛋白的增生，不同的光源会对皮肤产生不同的影响。

Methods The IL17 cDNA and Thy1.1 fulllength cDNA were subcloned into MIT(MSCVIRESThy1.1) retrovirus vector, and the siRNAIL17, U6 promoter and Thy1.1 fulllength cDNA were also inserted into retrovirus vector of pMNDBANSHEE.The recombined vectors were transfected 293 packaging cells by DNA calcium phosphate coprecipitation. Virus supernatant which infected preactivated spleen cells from NOD/BDC mice was collected. After incubation, the IL17 expression in diabetogenic T cells was detected.

利用基因工程和细胞克隆技术分别将IL17的cDNA插入MSCVIRESThy1.1逆转录病毒载体，将Thy1.1、U6增强子(U6 promoter)基因和IL17的siRNA cDNA插入逆转录病毒载体pMNDBANSHEE，采用磷酸钙沉淀法将重组载体转染293包装细胞；收集病毒上清，转染预先活化的NOD/BDC小鼠脾细胞，测定致糖尿病性T细胞的IL17和siRNAIL17的表达。

The apoptosis was evaluated by AQ-stained fluorescin, DNA ladder by gel electrophoresis and subdiploidy by propidium iodide-stained flow cytometry. The level of lipid peroxide under KI treatment was determined by measuring the production of thiobarbituric acid reactive substances.

取健康的猪甲状腺细胞培养24h后，加入不同浓度的碘化钾，观察细胞的形态改变；琼脂糖凝胶电泳、流式细胞术分析细胞DNA的降解；MDA法测定细胞膜的脂质过氧化程度。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。