细胞的

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明，所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达；在缺乏AMBN情况下，与对照组相比，实验组牙胚在体外可以继续生长发育至钟状晚期，出现成釉细胞和成牙本质细胞的分化，成釉细胞可以分化成为分泌期型成釉细胞，胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体，缺乏溶酶体，表明对蛋白合成和脚的能力降低；实验组牙胚有牙尖形成和基质分泌，但牙尖形态异常，基质形成减少，牙尖周围基质最厚处为O.6卜m，明显薄于对照组的5.spin，基质中胶原纤维粗细不等，排列稀疏， 3 第四军医大学硕士学位论文未见钙化现象，充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程，影响胶原纤维和牙本质基质的合成，促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

PART ONE CONSTRUCTION OF VECTOR CARRYING HUMAN INSULIN-LIKE GROWTH FACTOR-1 GENE AND THE GENE EXPRESSION IN EUCARYOTE Insulin-like growth factor-1(IGF-1) is one of the most important regulate factors which can stimulate cartilage cell to differentiate and synthesis collagen typeⅡand proteoglycan to maintain cartilage phaenotype.

第一部分：携带hIGF-1基因重组载体的构建及在真核细胞的表达胰岛素样生长因子-1(IGF-1)是在软骨发育和软骨自稳态调节中最重要的生长因子之一，能刺激软骨细胞分裂增殖，并促进软骨细胞合成Ⅱ型胶原和蛋白聚糖，维持软骨细胞的表型等。

Bone marrow derived fibrocytes migrate to the asthmatic airway from blood circulation acts as a progenitor cell of fibroblast and myofibrolast.

近来研究也发现，骨髓生成的纤维球扮演了纤维母细胞或肌纤维母细胞的前驱细胞角色，在特定细胞激素的引导下，由血流循环中移行至发炎的气道。

The positive expression rates of Tissue Inhibitors of Metalloproteinase-2 protein in the cases of involuting groups were significantly higher than those in proliferating group and vascular malformation groups and normal skin group (P.05). Its difference was significant. Conclusions: Matrix MetallProtinase-2 and Tissue Inhibitors of Metalloproteinase-2 may play an important role in the pathogenesis of hemangiomas through angiogenesis. They may have no effect on vascular malformation and normal skin

血管瘤退化期，TIMP-2表达明显升高，说明 TIMP-2与血管瘤纤维化、消退有关，其机制可能是一方面通过抑制MMP-2介导的基底膜降解而在内皮细胞迁移、聚集中起作用，另一方面直接抑制内皮细胞的增殖及迁移，使内皮细胞高频率调亡，细胞外基质增多，组织出现纤维化，最终导致血管瘤的退化。2、血管畸形和正常皮肤小血管中MMP-2和TIMP-2不表达或表达较弱，说明血管畸形和正常皮肤小血管中并不存在异常的血管生成，据此可以对血管性疾病进行分型、分期，并指导临床治疗。

It is concluded that the liposomal transfection of ASON inhibits the alphaglobin gene expression of K562 cells, which may be the new target for gene therapy in βthalassemia.

我们以K562细胞作为实验对象，用脂质体作为转染ASON的载体以增加细胞的吸收度及提高导入率，观察其对 K562 细胞α珠蛋白基因表达的抑制作用及对细胞增殖的影响。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

Using immunofluorescence staining with antibodies of specific enzyme of mast celland phalloidin. MC subset was defined by sorting and measuring of flow cytomery. Distribution of secretory vacuole and microfilament in MC were showed by laser scanning confocal microscope.

利用肥大细胞的特征性蛋白酶抗体和鬼比环肽的免疫荧光标记；以流式细胞仪检测分选人皮肤组织中肥大细胞的亚型；使用激光扫描共聚焦显微镜显示肥大细胞内分泌颗粒和微丝的分布。

Using immunofluorescence staining with antibodies of specific enzyme of mast cell and phalloidin. MC subset was defined by sorting and measuring of flow cytomery. Distribution of secretory vacuole and microfilament in MC were showed by laser scanning cofoncal microscope.

分泌方法：利用肥大细胞的特征性蛋白酶抗体和鬼比环肽的免疫荧光标记；以流式细胞仪检测分选人皮肤组织中肥大细胞的亚型；使用激光扫描共聚焦显微镜显示肥大细胞内分泌颗粒和微丝的分布。

By suspending cell culture from the callus of P. thugbergii and by fluorescent observation, a new method, which is easy and sensitive to observe cell activity and to test the phytotoxicity of various bacteria, was used. This method can be utilized to study the bacterial toxins. Cultured filtrates of some strains of the nematode-carrying bacteria were bioassayed for their toxicity to suspending cultured cells from P. thugbergii callus. Strong toxic effects were observed by the filtrates of Pseudomonas spp., P. fluorescence, and Sphingomonas spp., these bacteria species are coincide with those in the early stage of pine wilt disease.

本试验使用了黑松愈伤组织的细胞悬浮培养方法，用荧光方法观察各种细菌对植物细胞的毒性测定，这种方法具对细胞的活性观察容易、灵敏度高等特点，证明可用于细菌的毒素研究；对松材线虫携带的细菌的部分菌株，用其培养滤液对黑松愈伤组织的悬浮培养细胞的生物测定初步证明，松材线虫携带的细菌中以假单胞菌、荧光假单胞菌和少动鞘氨醇单胞菌的滤液对黑松细胞具有较强的毒性，这与松树发病早期出现的细菌种类相一致。

Result: With a high cancer-inhibiting rate on the animal model having been transplanted the cancer, this medicine can remarkably restrain the growth of cancer cells cultivated in vitro and improve the life quality of mouse model, and also shows obvious promotion and enhancement function on the DTH ear-swelling of mouse with cancer, the proliferation of mouse spleen lymphocyte induced by ConA and the cytokine (TNF-α、IFN-γ) of mouse with cancer respectively. It can obviously decrease the adverse effects like weight reduction, damage of viscera and degradation of life quality caused by the chemotherapeutic medicines, and has a remarkable stimulation on the lymphocyte proliferation induced by ConA. It is also able to improve the ratio of SMMC-7721 cells in G0-G1 period and reduce the cell amount in G2/M period to cut down the possibility of stepping into the division period; additionally, it can obviously inhibit some cancer genes of the cancer cells and meanwhile up regulate the cancer-inhibiting gene.

结果：本品能明显抑制体外培养肿瘤细胞的生长，对在体肿瘤移植模型动物具有较高的抑瘤率，能明显提高模型小鼠的生存质量；对荷瘤小鼠DTH耳肿胀、ConA诱导的小鼠脾淋巴细胞增殖、荷瘤小鼠细胞因子（TNF-α、IFN-γ）呈现明显的促进或提高作用；能显著减少化疗药所引起的小鼠体重降低、脏器损害以及生存质量下降等不良作用；本品含药血清对ConA诱导的淋巴细胞增殖具有明显的促进作用；能提高SMMC-7721细胞G0-G1期比例，降低G2/M期细胞数目，而减少其进入分裂期；对肿瘤细胞的多个癌基因具有明显的抑制作用，同时上调抑癌基因等。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。