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Restrictive changes of TCR Vp repertoire and clonal expanded T cells could be found in peripheral blood T cells from patients with T-ALL. However, the clonal expanded T cells' property (clonal expansion leukemic cells or leukemia antigen-specific expansion T cells) should be further characterized. It may play a certain role on detection of minimal residual disease and design of anti-leukemia idiotypic vaccine. The dominant utilization of TCR VjJ repertoire could be found in peripheral blood T cells from patients with B-ALL, meanwhile clonal expansive T cells were existed. It may be a feature of the host immune response for leukemia-associated antigen. The clonal expansive tendency of T cells in V021 and VP23 subfamilies was rather obvious, which may be correlated with certain malignant B-cell clone. The CDR3 sequences of monoclonal expansive T cell in T cell strains were different, which was the base of developing DNA vaccine.

T-ALL患者外周血T细胞的TCR Vβ谱系出现限制性改变,均可检测到克隆性增殖T细胞,尚需进一步鉴定其性质(肿瘤性或抗原特异性增殖),对于研究微小残留病变检测和设计抗白血病独特型疫苗均有一定的意义。B-ALL病人外周血T细胞的TCR Vβ谱系呈现优势利用的特点,并存在克隆性增殖的T细胞,这可能是机体对白血病相关抗原产生的特异性免疫反应;Vβ21和Vβ23亚家族T细胞发生克隆增殖改变的趋向性比较明显,可能与B-ALL相关抗原刺激有关。T细胞株中检测到的肿瘤克隆其CDR3序列具有高度的特异性,是构建DNA疫苗的基础。

Increase the bone marrow cell component while reduce the fat component, abnormal the caryon and cytomembrane structure of bone marrow cell and stromal cell, enrich the ribose body, mitochondrion and rough surfaced endoplasmic reticulum in cytoplasm of several organs and tissues, embody abvious improvement of heart muscle, liver, immnune organs, incretory organs and sexual gland.

增加免疫介导型再障小鼠的骨髓细胞成分,减少脂肪成分。使骨髓细胞和基质细胞的细胞核和细胞膜结构趋于正常,使多种脏器组织的细胞浆内核糖体丰富,线粒体及粗面内质网发达。对心、肝、免疫器官、内分泌器官及性腺等亦有明显的改善作用。

In conclusion, features of morohopathology, IHC profile and ultrastructural findings reveal obvious divergence between CHP and HP and resemble MP, so we suggest that histogenesis of CHP is pericyte with myoid differentiation.

根据此研究之结果得知,无论在肿瘤发生部位、组织病理形态学、免疫组织化学染色结果以及超显微结构的特徵,CHP与HP具有明显的差异性,反而与MP之特徵较为相近,此结果可间接证实CHP之肿瘤细胞来源与MP的肿瘤细胞之来源较为相似,可能为血管周围细胞来源并具有类肌肉细胞的分化。

The results showed that:(1) Many proliferating cells were distributed unevenly in the ventricular zone of telencephalon, and the [3H] labeled cells were found to be populated in the lateral ventricular zone of neostriatum at brain level L12~L14,forming proliferation "hot spots";(2) Having underwent mitosis twice, the proliferative cells in the VZ began to migrate laterally 6 days after intramuscular injection of [3H] thymidine, and these migrating precursor cells terminated at their targets and differentiated into new neurons on the 30th day.

结果发现:①端脑室带区中存在不均匀分布的[3H]标记细胞,大量标记细胞分布在端脑尾侧新纹状体水平(L1~L14)的VZ,形成标记细胞的&热点&区;②标记细胞经两次有丝分裂后,大约在肌肉注射[3H]的第6天后开始从VZ向外迁移,在30天左右到达目的脑区并分化成为成熟神经元。

Cells were collected to undergo HE staining and immunocytochemistry reaction against Thy-1 to identify RGCs. Axons length of retinal neurons and RGCs were measured by microruler under microscope respectively to evaluate the neurotropic effects of B vitamins in improving the regeneration and growth of axons for retinal neurons and RGCs. Finally, we observed the reaction of retinal neurons when exposed to the insult of glucose deprivation. The neuroprotection of B vitamins were also evaluated at the same time.

对B族维生素的神经营养和保护作用的研究过程分为三个阶段,首先确定维生素B族对视网膜神经元是否有神经营养作用,并根据细胞活力找出最佳有效作用浓度;然后在接种细胞的即刻用各种维生素的最佳有效作用浓度作用于视网膜神经元,并在不同时间终止培养,收集细胞,HE染色和RGCs的免疫细胞化学染色,分别测量视网膜神经元和RGCs的轴突长度,评价维生素B族是否有促进视网膜神经元和RGCs轴突再生伸长的神经营养作用;最后,观察视网膜神经元对低糖损伤的反应,及维生素B族对受损的细胞是否有保护作用。

A few nonadherent cells in the culture system couldn't interfere in the generation of DC. The structure of"veil"of DC made of graded dendritic projections twiested enlarged the area of membrane and became the foundation of structure to capture antigens.

四、结论: 1。该方法可以诱导出的成熟DC细胞,形态典型,而且少量悬浮细胞不影响DC细胞的分化和成熟;&面纱&状结构是由多级树突状突起相互缠绕形成,扩大了DC细胞的膜结构,是有效捕获抗原的结构基础; 2。

This experiment first studied in the mature nutrient medium to increase the hormone to the porine ovocyte in vitro mature process in the chromosome configuration and the mature rate influence, by explored the porine ovocyte in vitro mature rule, enhanced in vitro raise maturity, was the ROSI technology system preparation high quality ovocyte; Then has carried on the experiment in view of the porine round sperm cell in vitro separation, through to the ROSI operation and ICSI (Intracytoplasmic sperm injection, the comparison which ICSI) operated studies has affected the ROSI operation the factor.

本试验首先研究了成熟培养液中添加激素对猪卵母细胞体外成熟过程中染色体构型和成熟率的影响,以探索猪卵母细胞体外成熟规律,提高体外培养成熟度,为ROSI技术体系准备优质卵母细胞;然后针对猪圆形精细胞的体外分离进行了试验,进而通过对ROSI操作和ICSI(Intracytoplasmic sperm injection,ICSI)操作的比较研究了影响ROSI操作的因素。

Objective To explore the significance of DNA ploidy and cell proliferous ratio of prostate intraepithelial neoplasia in diagnose and differential diagnose.

目的探索DNA倍体类型、细胞周期在前列腺上皮内瘤中诊断和鉴别诊断的价值。方法应用细胞图像分析技术检测前列腺上皮内瘤细胞的平均DNA含量、DNA平均倍体值及细胞周期的各个时相的变化。

A lot of organelles such as mitochondria, rough endoplasmic reticula and Golgi bodies were observed in fiber cell. Then, double karyotheca disappeared, and the organelles disintegrated. Multivesicular bodies appeared in fiber cytoplasm. With the further development of fiber, fiber wall underwent continual thickening with aging, and polylaminate structure gradually appeared. While the agglutinated nucleus, transfer vesicles, plasma membrane and plasmodesmata still presented.

研究发现,次生壁形成早期,细胞核具有双层核膜,染色质凝聚,可见大量的线粒体、粗面内质网和高尔基体等细胞器存在于纤维细胞中;随后,双层核膜消失,细胞器将逐渐解体,多泡体开始出现在纤维细胞的细胞质;随着年龄的增加,纤维细胞壁逐渐增厚,并出现多层结构现象,而运输小泡、细胞膜、胞间连丝和凝聚的染色质将持续存在。

Their structures are different, the cytoplasm of the distal retinular cells is more compact than that of the proximal cells, but the numbers and kinds of the organelles of the distal retinular cells are less.

两部分的小网膜细胞在结构上有明显差异,上部的4个小网膜细胞的胞质比较致密,但是细胞器种类及数量较下部的小网膜细胞少。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。