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METHODS We used a dermatome to obtain thin endocardium,midcardium and epicardium transmural strips from left ventricular free wall of canine(weight 15-18 kg) and standard microelectrode techniques to record and measure the transmembrane action potential duration.

结果:①胺碘酮延长内、外膜层心肌细胞的动作电位时程,当基础周长为1 000 m s时,使用胺碘酮后心内膜层肌细胞的APD90由(254±38)m s延长为(274±37)m s(P.01),外膜层肌细胞的APD90由(205±37)m s延长为(229±16)m s(P.01);但其明显缩短中层肌细胞的APD,其APD90由(331±50)m s缩短为(290±62)m s(P.01)。

OBJECTIVE: To establish an inducing system for differentiation of bone marrow mesenchymal stem cells into chondrecytes in two-dimensional culture in vivo, to analyze the best concentration of transforming growth favor beta-1 (TGF-β1) to induce the differentiation and the correlated influence factors for the directional differentiation, and to observe the changes of cell form and phenotype.

目的:建立二维培养条件下骨髓间充质干细胞分化为软骨细胞的诱导体系,分析转化生长因子β1作为诱导条件的最佳浓度,以及诱导骨髓间充质干细胞定向分化为软骨细胞的相关影响因素,观察细胞诱导后的细胞形态及表型变化。

OBJECTIVE: To establish an inducing system for differentiation of bone marrow mesenchymal stem cells into chondrecytes in two-dimensional culture in vivo, to analyze tbe best concentration of transforming growth favor beta-1 (TGF-β1) to induce tbe differentiation and the correlated influence factors for the directional differentiation, and to observe the changes of cell form and phenotype.

目的:建立二维培养条件下骨髓间充质干细胞分化为软骨细胞的诱导体系,分析转化生长因子β 1作为诱导条件的最佳浓度,以及诱导骨髓间充质干细胞定向分化为软骨细胞的相关影响因素,观察细胞诱导后的细胞形态及表型变化。

It has little inhibition to the expression of Lamina and LaminB using Hematoporphyrin alone. The article basically involved the influence of cytoskeletal proteins after SDT. It also indicated the mechanism of cell necrosis and apoptosis and provided some data for SDT.

本论文在前期实验的基础上,初步研究声动力学疗法对S180肿瘤细胞的细胞骨架的影响,探讨其杀伤或诱导细胞凋亡的细胞生物学机制,为声动力学疗法治疗肿瘤积累相关资料。

Glucokinase, also called hexokinase Ⅳ, which catalyses glucose phosphorylation in hepatocytes and pancreatic β cells, is a key component of the glucose sensing machinery in mammals and plays a fundamental role in glucose homeostasis.

葡萄糖激酶(Glucokinase,GK)即Ⅳ型己糖激酶(Hexokinase,HK),主要作用是催化肝细胞和胰岛β细胞内的葡萄糖磷酸化为6磷酸葡萄糖,该步反应是细胞内葡萄糖代谢的初始反应,也是限速步骤之一,因此葡萄糖激酶又称为细胞的葡萄糖感受机制,在维持血糖稳定方面起着关键的作用。

The concept of cell differentiation; tissue-specific genes; and house-keeping gene; combinational control arouse expression of tissue-specific gene; the factors which have influence on cell differentiation; totipotency; cell differentiation and embryo development.

细胞分化的基本概念;组织特异性基因与管家基因;组合调控引发组织特异性基因的表达;影响细胞分化的因素;细胞的全能性;细胞分化与胚胎发育。

BACKGROUND: The technique of isolation and culture of human scalp dermal papilla cells is basic to study hair follicle biology. At present,microdissection to isolate the dermal papillae and culture DP cells is a primitive method,which has many drawbacks,such as laboriousness,low efficiency,uneasy adhesion and poor growth.

背景:毛乳头细胞的分离培养技术是研究毛囊细胞生物学的基础,目前采用的显微解剖法分离毛乳头进行毛乳头细胞传代培养是一种原始的方法,存在着分离效率低、劳动强度大、毛乳头贴壁率低、细胞生长缓慢等缺点。

Both the raising and lowering of temperature could inhibit the growth of rBHK cells compared with 37℃. More severe inhibition was observed when lowering temperature. The growth inhibition by temperature change derived mainly from the extension of lag phase, and specific growth rate of rBHK cells was almost independent on the change of temperature for rBHK cells in logarithmic phase.

相对于37℃培养温度,升高温度和降低温度对细胞生长都存在一定程度的抑制,降低温度培养条件下的抑制作用更明显,主要表现为延长了细胞生长的迟滞期,在细胞生长进入对数生长期后,培养温度对细胞的生长影响则不明显。

Methods LILs were isolated from the liver tissue samples from 11 CSHB patients and 5 normal controls by the method of combined grinding with semi-frosted microscopic slides and sedimentation of hepatic cells.

方法通过毛玻璃研磨加自然沉降肝细胞的方法,对11例慢重肝接受肝移植患者的 LILs 进行提取、分离。通过流式细胞仪测定慢重肝患者 LILs 及外周血中免疫活性细胞的频率并行对比分析,同时与正常人 LILs 及外周血中免疫活性细胞的频率进行对比分析。

Methods 40 healthy adult mice were randomly divided into control group, CTX immune-suppressed group, Fb treated group, Fb combined with CTX treated group. The influences of Fb in NK cell activity were determined by the method of NK mediated cytotoxicity test;the multiplicative capacity of lymphocyte was assayed by lymphocyte transformation rate of T cell,and the mice spleen T lymphoblast multiplication analytical method was used to measure the IL-2 level.

健康成年小鼠40只,随机分为对照组、环磷酰胺免疫抑制组、黄蘑碱溶性多糖活性组分正常小鼠给药组和Fb环磷酰胺伍用给药组,采用NK细胞介导的细胞毒试验测定自然杀伤细胞的活性,淋巴细胞转化试验测定T淋巴细胞增殖功能,鼠脾T淋巴母细胞增殖分析法测定白细胞介素2(IL-2)的活性,观察Fb对各组小鼠免疫指标的影响。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。