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Results AQP1 is expressed at the apical and basolateral membrane of the microvascular endothelium; AQP3 was detected at basal cells of both the bronchiole epithelium and submucosal gland acinus; AQP4 is present in the basolateral membrane of columnar cells in bronchiole; while AQP5 is expressed in the apical membrane of type Ⅰ pneumocytes, and also at the apical of columnar cells of superficial epithelium and submucosal gland acinar cells.

结果本研究发现AQPs基因在羊肺中的表达分布与人相似,AQP1在肺内的毛细血管内皮细胞表达;AQP3在小支气管黏膜上皮的基底细胞的基侧膜表达,AQP4存在于小支气管黏膜上皮的柱状纤毛细胞的基侧膜;AQP5存在于Ⅰ型肺泡上皮细胞的顶质膜,存在于小支气管黏膜上皮柱状纤毛细胞,以及在气道黏膜下腺的腺细胞的顶质膜表达。

The histiological characteristcs, and activities of alkine phosphatase and acid phosphatase of the implantan were examined to study the effects of fluoride on biological activities of BMP.

结果 氟对BMP的生物活性有直接的影响,氟能促进BMP将更多的成纤维细胞转化为成骨细胞,并能增强成骨细胞的功能,加速钙盐沉积,而破骨细胞功能有所下降。

Epithelial cell defenses include the ciliated epithelial cells, goblet cells, and other secretary epithelial cells of the tracheobronchial tree

表皮细胞的防御包括起纤毛作用的表皮细胞、环状细胞和气管支气管树的其他起分泌作用的表皮细胞。

Epithelial cell defenses include the ciliated epithelial cell s, goblet cell s, and other secretary epithelial cell s of the tracheobronchial tree

表皮细胞的防御包括起纤毛作用的表皮细胞、环状细胞和气管支气管树的其他起分泌作用的表皮细胞。

Results When Raji and B95-8 cells were cultured with 20-80 μg/ml of "Dongxia wan", the expression of EBV early antigen and EBV capsid antigen induced by croton oil and n-butyric acid and the natural expression of EBV-VCA were all inhibited significantly. The inhibition rates were 19.3%-49.84%, 34.63%-45.61% and 21.67%-47.78% respectively.

结果 20~80 μg/ml中药复方"冬夏丸"与靶细胞共培养能显著的抑制巴豆油和正丁酸联合诱发Raji细胞的EBV早期抗原和B95-8细胞的EBV壳抗原表达及B95-8细胞的EBV-VCA自然表达,抑制率分别是19.31%~49.84%,34.63%~45.61%和21.67%~47.78%。

Flow cytometry techniques was used to to determine cell apoptosis under the action of mifepristone. And expression of survivin gene was evaluated by reverse transcription polymerase chain reaction. Result: Four different condensations dose-dependently inhibited the proliferation of HO8910 cells.the inhibition by mifepristone plateaued at 20 y.

将HO8910细胞进行体外培养,采用MTT法测定,观察不同浓度米非司酮(20,10,5,2.5μmol/L)对HO8910细胞的杀伤活性;通过流式细胞仪分析用药后细胞凋亡的情况;逆转录PCR法检测了米非司酮培养前后Survivin扣只人A的表达,并将结果进行了相关分析。

objectiveto investigate the effects of yuegan capsule on ccl4 intoxicated primary cultured rat hepatocytes.methodshepatocytes injury models were induced by ccl4 in rats in vitro, and treated with yuegan capsule.structure of rat hepatocytes and effect of sdh、acpase、g 6 pase activeties and amount of hepatic glycogen were observed.

目的探讨悦肝胶囊对四氯化碳(ccl4)损伤原代培养大鼠肝细胞的保护作用。方法以ccl4诱导大鼠肝细胞损伤模型,观察悦肝胶囊对肝细胞的琥珀酸脱氢酶、酸性磷酸酶、葡萄糖-6-磷酸酶(g 6 pase)活性和糖原含量以及肝细胞形态结构的影响。

However, when two maturing oocytes fused and two spindle will form in the big cell, the chromosomes will not intermingle. In the present study, we removed GV from one oocyte and transfer to the perivetelline space of another GV oocyte. After fusion the resulting oocytes which contained two GVs were cultured further in MEM.

在本实验中,我们利用小鼠GV期卵母细胞将一枚卵母细胞的生发泡取出后移至另一未经去核处理的GV期卵母细胞透明带下,经三次电脉冲作用后将融合的含有两个GV的卵母细胞放入MEM成熟培养液中培养,在培养成熟的不同阶段分别收集卵母细胞进行免疫荧光染色观察微管及核的变化。

The results showed that after 2 h of culture in MEM the chromosomes of oocyte were seperated to form telophase I while a small spindle was observed around chromosomes of primary spermatocyte. However, two clear spindles were observed in the oocytes cultured in CB containing MEM. After further culture, the chromosomes of both primary spermatocyte and oocyte intermingled and formed one large spindle.

在无CB的培养液中培养的卵母细胞培养2小时后,卵母细胞已经进入第一次减数分裂的后期,染色体开始被拉向两极,而精母细胞的MI纺锤体才刚刚形成,虽然继续培养两者染色体可以合二为一并形成一个纺锤体,但是有些染色体发生滞后;当卵母细胞在含有CB的培养液中培养2小时后,在卵母细胞内形成两个相似大小的MI纺锤体,进一步培养形成一个大的纺锤体,染色体正常。

Bcl-2 mRNA was detected by RT-PCR in all cells;(2)After normal rat and bile duct-ligated 14d rat hepatocytes were added 100 μmol/L GCDC and kept for 24 hours, cells were evaluated by FCM and TUNEL. Results:(1)Normal rat hepatocytes did not express Bcl-2 by RT-PCR technique. Bcl-2 was expressed in 7-,14-,21- day BDL rats.

分离对照组及实验组14 d组大鼠的肝细胞行原代培养后,使用100 μmol/L甘氨鹅脱氧胆酸钠作用于两组肝细胞24 h后,用FCM法测定肝细胞的凋亡比率,用TUNEL技术进行肝细胞凋亡的原位检测。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。