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At 4 wk, the cells in the liver bud migrated into the domain of transversum mesenchyme, where endothelial cells began to form vascular structures, and branched to form a number of hepatic cords.

结果:3wk时靠近肝芽的间充质中的血管内皮细胞多,而肝芽内未见血管内皮细胞及造血细胞:4wk时,肝索形成处可见较多正在形成的新生血管增多,肝索间开始出现少量的造血细胞:5wk时肝索间出现原始的肝血窦结构,血窦内造血细胞的数量较4wk明显增多。

It was possible that the direct transport of JA from unwounded tissue to wounded site could account for the increase of JA after leaf wounding.

用免疫荧光显微镜技术和免疫胶体金电镜技术证明JA在蚕豆叶片下表皮和气孔保卫细胞上是普遍存在的,它主要分布在保卫细胞的叶绿体、细胞核和细胞壁上;叶片的局部灼伤可提高JA在质外体和气孔保卫细胞中水平。

The incompatible results have been reported even with the same methods But these studies were carried out on the basis that the structure of nucleolus is regarded as a static state. However, the nucleolus is a highly dynamic structure during the cell cycle. It disassembles in the late prophase and reassembles at the end of mitosis.

不过以前对核仁结构和功能的研究大多只是对细胞间期中某个时期细胞的核仁进行静态研究,然而核仁是一个高度动态变化的结构,在细胞分裂前期解体,在细胞分裂末期又开始重建。

This model suppled a reliable pathological cell tool for the study of atherosclerosis .

本实验从离休细胞培养方面探寻建立小鼠巨噬细胞源性泡沫细胞的可能性,为动脉粥样硬化的形成机理分析和防治研究提供了一种可靠的病理细胞模型。

Results VEGF-C and VEGFR-3 are all expressed on both mesenchymal cells and vascular endothelium of the embryos during early period. In middle and latter period, VEGFR-3mRNA is mainly expressed on cell plasm of alimentary canal, but it has no expression on vascular and lymphatic endothelium; The expression of VEGFR-3 is extensive and positive expression is seen on the vascular and lymphatic endothelium.

在胚胎中、后期消化道组织中VEGF-C主要表达于消化管壁组织细胞以及肠系膜内,在血管和淋巴管内皮细胞则无表达;VEGFR-3亦表现为广泛的表达,于血管和淋巴管内皮细胞可见阳性表达,并随着胚胎的发育,其在淋巴管内皮细胞的阳性表达率明显增加。

Results No effect of tomato juice on the growth of human normal cells and tumor cells when the proportion of tomato juice in cell culture fluid under 15%; cell growth was inhabited when the proportion of tomato juice in cell culture fluid was up to or higher than 20%.

结果 当番茄汁体积在培养液中所占比例低于15%时,对正常细胞和肿瘤细胞的增殖没有任何影响;当番茄汁在培养液中所占比例达到或超过20%时,此时的培养液不是细胞生长的最适条件,表现为细胞生长抑制。

The organization fixing of apoenzyme subtypes by IHC: The most increasing expressing were cPLA_2 and sPLA_2 in pancreas, they were found all over in the pancreatic acinar cells in 6h after PT, and spread out from the cells in 24h,then decreased after 72h.

用IHC技术对酶蛋白亚型组织定位方面:胰腺内的表达以sPLA_2和cPLA_2增加为主,6h时即遍布于胰腺腺泡细胞;24h时由于胰腺细胞的大量破坏,腺泡细胞外均可见sPLA_2和cPLA_2的高表达;72h后细胞内外sPLA_2和cPLA_2逐渐下降。

Three conditions of spermatogenic cells coculture with Sertoli cells in vitro were studied in the third experiment.

通过观察在这三种不同培养条件下生精细胞体外共广西医科大学硕士学位论文生精细胞体外培养的研究培养的生长行为、培养前与培养后不同时间内细胞的存活率以及生精细胞体外分化情况,进行统计学分析比较。

Methods The astrocytes were isolated from cerebral cortex of neonatal rats which are born within three days, cultivating by DMEM media added with 15% fetal calf serum and depurating by swinging and passaging, to simulate nulli-nutrition model by substituting medium for PBS buffer solution 3h then applying methylprednisolone and recnutrition immediately, we will go on culturing for three days, verificating astrocytes by immunofluorescent technique; detecting activity of AST; determining GFAP of change by PCR.

采用出生3d内的SD大鼠的新生鼠的大脑制成细胞悬浊液后,应用含15%胎牛血清的DMEM培养液中培养,通过摇床和逐渐传代纯化星形胶质细胞,通过PBS缓冲液代替培养基模拟缺营养模型3h后即刻应用甲基强的松龙(10umol/l),然后继续培养3d,免疫荧光技术鉴定星形胶质细胞;MTT法检测细胞的增殖活性和凋亡;PCR技术测定细胞GFAP表达的变化。

After introduction of the recombinant plasmid into BME cells, the capability of proliferation was obviously enhanced and the DNA content was changed, the cell proliferation lifespan was elongated to 13 population doublings to control.

导入STAT3基因后,细胞增殖能力增强,染色体倍数发生变化,细胞的增殖寿命较对照细胞延长了13代,表明导入STAT3基因后,能延长体外培养的牛乳腺上皮细胞的寿命。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。