细胞的

Preadipocytes from epiploon of newborn calf were cultured by monolayer adherent method, and total RNA of the preadipocytes was extracted on day 4,6,8,10,12,14 and 16 post-culture, respec-tively. The abundance of ADPN mRNA and HSL mRNA was detected by real-time fluorescent quanti-tative RT-PCR during differentiation period from preadipocyte to adipocyte.

选取犊牛大网膜脂肪组织，进行牛前脂肪细胞的单层贴壁培养，在细胞接种后第4、6、8、10、12、14、16d分别提取细胞总RNA，采用实时荧光定量RT-PCR方法，检测了体外培养的犊牛前脂肪细胞不同时期ADPN基因mRNA和HSL基因mRNA的表达情况。

Methods Corneal epithelial cells of limbal, peripheral and centeral cornea were removed using improved enzymatic disaggnegation and cultured in vitro. The cells were counted in a hemocytometer at different time to determine the doubling time .

用体外细胞培养的方法，从生长曲线、细胞倍增时间、细胞DNA合成的活跃程度等几个方面，观察人角膜缘部、周边部及中央部角膜上皮细胞的生长特征。

Researchers have had systematically studied on this technique for many years, and this technique has been utilized in application and made a lot of effects;2 Combining with the techniques of cell engineering (nuclear transplantation and electric fusion etc.), and through the process of culturing histiocyte of fish, cryopreservation and re-culture after thawing, carrying out somatic cell breeding of fish.

多年来，国内外学者对各种鱼类精液的冷冻保存进行了大量的系统研究，目前这项技术已达到实用水平，并日益发挥作用；二是对鱼类培养的组织细胞冷冻保存，通过鱼类细胞的培养、超低温冻存、解冻后再培养过程，结合细胞工程技术进行体细胞育种；大量的研究结果表明鱼类体细胞核具有发育的全能性，随着细胞培养技术、细胞工程技术日益发展成熟，完全具备实现鱼类物种种质长期保存的理论基础和技术条件。

Treated with high concentration of piperine （0.56 mmol/L） for 24 hours and 48 hours respectively, following a period of growth retardation, the cells of 24-hour-group could recover slowly while the 48-hour-group showed inconvertibility.

由此认为：胡椒碱对C6/36细胞株有毒性作用，但药物诱导的细胞凋亡在细胞毒性作用机制中不占主导地位；胡椒碱对C6/36细胞的作用有时间依赖性，延长作用时间，药物对细胞的毒性作用增强。

Results ERα immunoreactivity was detected in the nuclei of epithelial cells lining lobules and ducts.The positive cells located in the inner layer of the two epithelial layers in the lobules and intralobular ducts,but in the interlobular ducts the positive cells located in the outer layer.

结果：ERα在小叶上皮细胞和导管上皮细胞的细胞核内检测到，在小叶和小叶内导管分布于上皮细胞的内层，在小叶间导管分布于上皮细胞的外层。

Methods: Study the newly found cell with the weak silver carbonate staining method of del Rio-Hortega. Using transmission electron microscopy, compare the newly found cell with the atypical cell found by Marc Lenoir and Philippe Vago, and extend young SD rat model to adult SD rat, young and adult Wistar rat,and extend drug of Neomycin to Amikacin to study the universality.

采用经典的小胶质细胞特殊染色方法——银染法(Hortega氏碳酸银法)对大鼠耳蜗药物损伤后出现的新细胞进行研究；通过透射电镜与Marc Lenoir和Philippe Vago实验动物模型中的"非典型细胞"进行了比较与扩展研究；运用逆转录PCR技术检测耳蜗基底膜上神经干细胞标志物nestin的表达情况，对新细胞的来源及其与神经干细胞或nestin的前体细胞的关系作进一步研究。

Therefore, in order to find out the most important bioactivity of Hoshouwu, the present research chooses cell regeneration and immunomodulation as platforms for validation. The bone marrow cavity consists of two rapidly regenerating systems: hematopoiesis and bone remodeling process.

骨髓是人体持续进行细胞再生作用最活跃的区域之一，本论文分别以体外细胞培养及动物实验方式测试何首乌成分对骨髓中具有细胞再生功能的两大族群：造血干细胞和基质细胞的相关影响。

Mast cells and macrophages may be involved in the development of PH and/or the lesion caused by PH accelerate the accumulation and activation of mast cell and macrophages.

结论肥大细胞及巨噬细胞参与了肺动脉高压的形成或/和肺动脉高压造成的损伤促进了肥大细胞及巨噬细胞的聚集与活化。

A micropipette aspiration technique was employed to investigate the adhesive properties of hepatocellular carcinoma cells to collagen type Ⅰ, based on comparison between single cells isolated from the primary culture of human fetal hepatocytes and from the culture of HCCs.

重庆大学生物工程研究院，重庆 630044本文以正常胎肝细胞的原代培养及肝癌细胞的传代培养为基础，应用微管吸吮技术在单个细胞水平上研究肝实质细胞癌细胞在胶原蛋白Ⅰ裱衬表面的粘附力学特性。

PartⅠ/chapter 3 The viability of cultured cerebral endothelial cells was determined by measuring the release of intracellular enzyme lactate dehydrogenase by nonviable cells with increased permeability.

第一部分第三章乳酸脱氢酶（1actate dehydrogenase，LDH）是一种细胞内酶，当细胞失去活力膜通透性增加时即释放到细胞外。本文通过测定释放到培养液中的LDH的量来测定牛脑微血管内皮细胞的活力。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。