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It is suggested that the stratum intermedium participate in the differentiation of ameloblast and formation of enamel.

中间层细胞的增殖和凋亡及其TNSAP的表达与成釉细胞具有相关性,提示中间层细胞有可能参与成釉细胞的分化及釉质形成。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling . Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied.

在研究过程中,采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术,系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化,T和B淋巴细胞、T淋巴细胞亚群的动态变化规律,淋巴细胞表面某些粘附分子的变化,淋巴细胞和神经元的坏死与凋亡,雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律,脑组织中围官性细胞浸润的细胞类型。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling. Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied. The key research results were.(1)The average percentage of CD19+cell in blood, bursa and spleen, after 3, 5 days of inoculation, was significantly Phybridization revealed that the number of IL-8 and MIP-1βproducing cells was increased in the infected brain.

在研究过程中,采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术,系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化,T和B淋巴细胞、T淋巴细胞亚群的动态变化规律,淋巴细胞表面某些粘附分子的变化,淋巴细胞和神经元的坏死与凋亡,雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律,脑组织中围官性细胞浸润的细胞类型。

Subcellular localization of ATPase activity in the flesh of developing apple fruit was carried outusing a lead phosphate precipitation technique via electron-microscope. The ultrastrcture of phloemand its surrounding cells was also observed. The results showed that ATPase activity wasdominantly localized on the plasma membranes of sieve element/companion cell complex.

应用铅沉淀的细胞化学方法,研究了发育过程中苹果果肉内卸载区(韧皮部及其周围薄壁细胞)ATP酶的亚细胞分布和变化,并且,作为补充,观察了韧皮部及其周围薄壁细胞的超微结构。

These"cross-talk"may also provide useful information on regulation of mucosal immune response.

在体外建立免疫细胞与上皮细胞共培养技术是研究免疫调节对上皮细胞功能影响以及上皮细胞的免疫调节作用的一种实验技术,是免疫生理学研究的一个突破。

Exogenous E2 alone has no effect on cell viability and apoptosis of H9C2 cells, but it can decrease the sensitivity of H9C2 cells to H_2O_2,hence,protect cells against apoptosis caused by oxidative stress.2Exogenous E_2 has downregulation effect on TβRⅠand TβRⅡof H9c2 cells,blocking ER may delete this effect of estradiol.H9c2 cells express ERβonly,suggests that E_2 may exert modulating effect on TGF-βpathway through ERβ.

外源性E2本身并不能影响H9c2细胞凋亡,但能够降低H9c2细胞对H_2O_2的敏感性,保护细胞抵抗氧化应激引起的凋亡。2外源性E2对H9c2细胞的TβRⅠ以及TβRⅡ具有下调作用,阻断ER可消除E2对TβRⅠ和TβRⅡ的下调作用;E2可进一步通过TGF-β通路降低受体后磷酸化smad2/3的水平;H9c2细胞仅表达ERβ,提示E2可能是通过ERβ发挥其对TGF-β通路的调控作用。

During denuded mice oocyte mature process in vitro, the cortical granulesmay have abnormal numbers or generation problems. This may be another reason thatmakes denude oocytes matured in vitro have a clearly deficiency on fertilization invitro.

3去除卵丘细胞的小鼠卵母细胞在体外成熟过程中,皮质颗粒可能存在继发性生成障碍或者数量异常,该结果可能是去除卵丘细胞的小鼠卵母细胞存在体外受精障碍的原因之一。

It is widely accepted that AP depolarize the plasma membrane and open voltage-dependent calcium channels , the calcium influx through VDCCs trigger the fusion of vesicles with plasma membrane and subsequent transmitter release.

现在被普遍接受的观点是当可兴奋细胞受到动作电位的刺激后,细胞的电压依赖性钙离子通道被打开,在动作电位的下降相引起细胞外钙离子的内流从而升高细胞内的游离钙离子浓度,触发许多细胞内的过程,譬如说神经递质分泌、信号转导和基因表达等。

RESULTS: Cells were spindle shape before induction, obviously varied following preinduction, and then the body began contracted after being induced in the medium for 1 hour, with the presence of a few small orbicular-ovate or spindle cells. Following 24 hours of induction, about 60% cells became double pole, multipolar or cone shape, showing neuron-like structure.

结果:①诱导分化前呈梭形细胞,在加入预诱导培养基后未出现明显的形态变化,换用诱导培养基1 h,胞体开始收缩,出现少数较小的卵圆形或纺锤形细胞,诱导24 h后,约60%细胞变成双极形、多极形和锥形,出现类似神经元细胞的形态。

At last, the classification method is discussed in accidence and a classification method based on decision-tree is presented. In the method, the characteristic parameters of cells are extracted through different methods such as the morphologic, chroma and texture of cells, and then normal cells are eliminated gradually by the means which screens out normal cells from roughly to accurately, and in the end suspicious tumor cells are sorted according to their dubiety.

最后,对细胞的分类方法进行了深入的研究,提出了一种基于判别树的层次分类方法,采用细胞的形态学、色度学和纹理等不同方法获取的细胞特征参数,采取"由粗到精、逐步筛选"的策略逐步剔除正常细胞,并根据可疑程度对疑似肿瘤细胞排序,辅助病理专家的诊断工作。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。