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Methods MSCs were obtained from tibias by density gradient centrifugation and were tested the rate of adhesion. The configuration was observed under phase-contrast microscope consecutively and the cell growth was measured by MTT method. Cell cycle and surface antigens were detected through flow cytometer.

测定其接种贴壁率,在倒置显微镜下连续观察细胞的形态变化,利用甲基噻唑基四唑(methyl thiazdyl tetrazolium, MTT)法测定 MSCs 生长曲线,并应用流式细胞术测定细胞表面标记物。

Then, TAMs inhibitory effect united with elemene on MCF7 cell line was analyzed by using CCK8 test.

接种细胞并加入实验药物,用CCK8法检测三苯氧胺联合榄香烯对细胞的增殖抑制效应,用酶标仪在450nm测量每孔吸光度值,计算每组细胞抑制率。

Then, TAMs inhibitory effect united with elemene on MCF7 cell line was analyzed by using CCK8 test.

接种细胞并加入实验药物,用CCK8法检测三苯氧胺联合榄香烯对细胞的增殖抑制效应,用酶标仪在450nm测量每孔吸光度值,每组细胞抑制率。

The number of CD4+CD25+ regulatory T cells (CD4+CD25+ Treg cells) in spleens was measured using flow cytometry.

同时, 采用流式细胞术分析其脾细胞悬液中CD4+CD25+调节性T细胞的数量变化。

The number of CD4+ CD25+ regulatory T cells (CD4+ CD25+ Treg cells) in spleens was estimated using flow cytometry.

同时, 采用流式细胞术分析其脾细胞悬液中CD4+CD25+调节性T细胞的数量变化

Rat primary hepatocytes cultured on chitosan microcarrier were observed by using phase contrast microscope and scanning electron microscope.

用其进行原代大鼠肝细胞培养,利用相差显微镜和扫描电镜对细胞形态进行观察,测定细胞的代谢活性。

Objective To investiga te the ability of dendritic cells ac quiring antigen from apoptotic glioma cells and inducing CTLs activati on.

目的 观察树突状细胞从凋亡脑胶质瘤细胞获取抗原后,体外诱导抗肿瘤免疫应答及对胶质瘤细胞的特异性免疫杀伤效果。

Different cells,cell density and cell shape lead to comparatively great difference in wood properties and physical mechanical properties, change of wood cells affects change of wood properties including wood quality,wood property and all other aspects,and wood properties decide economic value and directly affect manufacturing and utilization of wood,therefore,the research and analysis on wood cell structure become especially important.

由于树木生长不均匀,使各种树木的木材构造具有多样性,不同细胞体、细胞密度和细胞形状导致了木材材性和物理力学性能存在较大差异,木材细胞的变化影响着木材性质的变化,木材的性质又决定木材经济价值,并直接影响木材的加工和利用,由此对木材细胞结构分析研究就变得尤为重要。

Objective To observe the change of T cell second cluster and NK cell after exchange transfusion in newborn of hyperbilirubinemia and to provide theory foundation for exchange transfusion.

目的动态观察高胆红素血症新生儿换血前后,T细胞亚群及NK细胞的变化,为高胆红素血症新生儿换血疗法提供细胞免疫学基础理论。

Rabbit digestive tract were investigated by the method of immunohistochemical technique and histochemical technique (toluidine blue or neutral red staining). There was immunoreactive positive reaction in rabbit digestive tract except in esophagus part.

利用免疫组化技术和组织化学技术,对家兔消化道5-HT免疫反应阳性细胞、肥大细胞进行了研究,并通过邻片法比较探讨了2种细胞的对应关系。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。