细胞的

The study was done to compare the induction efficiency of human embryonic stem cells to neuroepithelial progenitor cells in human embryonic fibroblasts,mouse embryonic fibroblasts and feeder free systems.

中文摘要：［摘要］比较人胚胎干细胞在人胚胎成纤维细胞、鼠胚胎成纤维细胞饲养层和无饲养层3个诱导体系下形成神经上皮祖细胞的能力。

Nither CDMP-1, nor cryopreservation can effect cell cycle of the chondrocytes.

结果：残耳软骨细胞在体外培养时随代数增加呈异染性的糖胺多糖减少，第1、3代Ⅱ型胶原阳性细胞比例约68％/～71％，第5代为23％、显著降低；冻存后第3代为65％，与同代数的细胞没有差别，100ng/ml浓度的CDMP-1可以将第4代细胞的阳性比例维持在66％，而且冻存和生长因子不会引起细胞周期的变化。

Coli LLO/OVA as its creating pore activity helping OVA antigen escape from lysosomal vesicle of dendritic cells, and the bacterial content can then be processed by the proteosome and the antigen motifs presented onto MHC class I to CD8+ T cells, initiating stronger cytotoxic T cell immunity.

本实验选用LLO作为免疫佐剂，利用其穿孔素作用促OVA抗原从树突状细胞的吞噬溶酶体进入细胞浆，并经MHC-I类分子递呈给CD8+ T细胞，从而诱导较强抗肿瘤免疫。

The first passage cells were initially pured by enzyme digestion, then pured by enzyme digestion and repeated strapping alternatively in the second course of transferation and inoculation, the third period cultured cells showed typical clonali growth and were long fusiform fibroid cells with short growth cycle.

首次传代时应用胰酶消化法进行成纤维细胞的初次纯化，第2次转种时先后应用酶消化法和反复贴壁法再次进行细胞纯化，第3代以后基本可获得呈典型克隆性生长、长梭形纤维样细胞，生长周期短。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

The neurosecretory somata were located onthe proximal surface of the medulla terminalia of the eyestalk.Three types ofneurosecretory cells were distinguished on the basis of size, morphology,distribution,form of outgrowth and ultrastructural feature.

河蟹眼柄神经分泌细胞主要分布于眼柄视神经节端髓基部外侧，依据细胞的大小、形态、超微结构特征及离体生长形态区分出三种类型神经分泌细胞。

However in the preparation of ganglion-body wall, when the mechanical'toucher'was still on the body wall, the high frequence activity of AP eells could make the T and P cells discharge, but no synaptic potential could be found in the T and P cells at the same time. When the contralatcral root was cut, this kind of effects disappeared.

然而，在体壁-神经节标本上，当机械刺激物仍接触表皮时，AP细胞的高频发放使T和P细胞产生动作电位，但此时在T和P细胞上未见有突触后效应，切断神经根这种影响消失。

The etiology and pathogenesis is not very clear,may be due to excessive mechanical load or unbalanced distribution of cartilage wear,and then chondrocyte metabolic changes.Cell release a variety of catabolic enzymes that lead to aggrecan degradation,collagen network useless and cartilage unhomogeneity.

整合素作为细胞表面受体使整合素在细胞跨膜信号传递中起一定的作用，并且参与细胞的多种生理功能，如细胞的生长、发育、分化、凋亡等，同时参与多种病理过程，如淋巴细胞向炎症部位的聚集及肿瘤细胞的浸润等[2]。

Results: Compared with that treated with anti-thyroid drugs alone, after the combined therapy, the size of thyroid decreased significantly, P＜0.01. Typical apoptosis appearance, such as vacuolated cell, marginal nuclei, chromatin condensation and nuclear fragmentation, could be seen under light microscopy. The apoptotic rate before and after adding Chinese herbs was (2.11±1.78)%and (18.66±20.01)%respectively (P＜0.01). TUNEL positive cells were detectable for up to 2-20 weeks after Chinese herbal treatment.

结果：与单纯抗甲状腺药物治疗比较，患者经抗甲状腺药物与中药联合应用治疗2-10周后，甲状腺组织明显缩小(P＜0.01)；光镜下可见细胞发泡、核着边、核固缩、核小体、核分裂等凋亡细胞的典型形态；加中药治疗前后甲状腺细胞凋亡比率分别为(2.11±1.78)%和(18.66±20.01)%(P＜0.01)；末端标记呈阳性。

RESULTS:①Morphology of skeletal myoblasts: After 48-72 hours primary culture, the cells were completely attached and gradually extended in the shape of spindle with the round nucleus, paralleled along their longitudinal axis and appeared chromatospherite. After 5-day culture, the cells stretched out one or several apophyses.

结果：①骨骼肌卫星细胞的形态：原代培养48~72 h细胞完全贴壁，呈梭形或纺垂形，长轴平行排列，胞核圆形，核仁明显。5 d时细胞可伸出一个或数个突起。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。