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RESULTS: Positive immunohistochemical signals of MMPs and TIMPs could be found in fetal skin at different gestational periods.

结果 MMP -2、MMP -3、MMP -9、TIMP -1和TIMP -2在不同时期的胎儿皮肤内均有表达,它们主要分布于表皮细胞、成纤维细胞、毛囊和汗腺上皮细胞以及血管内皮细胞的胞浆中。

Methods Chromosome, HE stainings and autoradiography were made for the cornea of human and monkey.

目的 探讨灵长目动物角膜内皮细胞的再生能力及方式方法对猴及人角膜做染色体染色、HE染色及放射自显影结果成年创伤活体猴角膜内皮细胞有再生能力成年及幼年灵长目动物角膜内皮细胞在生理和病理条件下的再生方式均为无丝分裂结论灵长目动物角膜内皮细胞在一定条件下有再生能力,其再生方式为无丝分裂

This article is to detect the effect of rabbit sera on cell circle and DNA of Shope carcinoma cells with Flow cytometer.

用流式细胞仪检测兔血清对shope肿细胞的细胞周期及细胞DNA的影响。

Results:We hae screened out the best method of drawing the materials from OB,which is crushing directly. we also conside the immunoadsorption as the best method of puring OECS.Coating the petri dish is profitable to pure the cells. meanwhile the appropriate cells density inoculated is important for eentual purity of culture.

结果:我们从中筛选出最佳取材方法为直接挤压法,最好的纯化嗅鞘细胞方法为免疫吸附法,同时发现包被培养皿对纯化细胞意义重大,而高密度接种细胞对提高细胞的最终纯度也是非常有利。

Part III: Peripheral blood dendritic cells in coronary atheroscleroticdiseaseObjectives: Dendritic cells subsets, Myeloid DC and plasmacytoid DC, regulate immune reactions by polarising naive T-helper cells into a Th1 or Th2 response, respectively.

第三部分:冠心病患者外周血树突状细胞亚型的变化的研究目的:树突状细胞的两个亚型,髓样DC和浆细胞样DC分别是促使T辅助细胞向Th1和Th2免疫应答转化的抗原呈递细胞。

GFAP overexpessed but NOS neuron decreased, distorted and pale. The glia in SM-DB complex of ONR hypertrophied, but NOS neuron no degeneration. The increased GFAP immunoreactivity and the morphometric and cytological changes in SM-DB complex astrocytes may reflect a sustained upregulation of cellular activity with the insults of neurons, resulting in hypertrophy of glial perikarya and cell processes.

老年减退组星形胶质细胞的细胞体密度较老年正常组和青年组明显增加(P 在GFAP免疫细胞化学和NOS组织化学双重染色的切片上,老年减退大鼠SM-DB星形胶质细胞明显肥大,突起粗壮弯曲,GFAP超量表达,而NOS阳性神经元数量减少,胞体变形,突起减少,染色深度下降。

LDH in culture fluid, SOD, XOD, free calcium in L-6TG cell and mitochchondral respiration were evaluated in each group; apoptosis was detected by flow cytometer with PI staining method and agarose gel electrophoresis; the immuneohistochemical method was used to determine the expression of bcl-2 and Caspase-3 ; the morphoiogic changes were observed with microscope.

将培养的L-6TG大鼠肌母细胞随机分为3组:①正常对照组,②缺血再灌注组(1R组),③缺血预处理组,观测了培养上清中LDH、细胞内SOD、XOD、Ca(上标 2+)含量的变化;采用MTT法检测线粒体的功能;利用流式细胞仪和细胞DNA电泳结果检测细胞凋亡情况;采用免疫组织化学的方法检测bcl-2及Caspase-3的蛋白表达情况,结合自动图像分析系统对其结果进行定量分析;在光镜下观察细胞的形态学改变。

Compared with the sense group,the mRNA expression and the protein expression of HIF 1α and TGF 3β decreased in antisense group.

滋养细胞的多功能细胞因子TGF 3β表达,与滋养细胞增殖和分化有关,可调节滋养细胞的浸润功能。

The levels of E2F1 proteins could return to normal at 2h and 16h in high MOI infected cells treated with large dosage of allitridin.⑤The effects of allitridin on regulating the transcription and expression of dhfr gene in HCMV infected cells: The higher transcription of dhfr gene of high MOI infected cells within 4-16h was partially or wholely antagonised by allitridin. The up-regulated dhfr mRNA levels of low MOI infected cells nearly returned to normal after allitridin treatment.

大蒜新素处理HCMV感染细胞对E2F蛋白表达的影响:大蒜新素处理低MOI和高MOI感染细胞可使病毒诱导上调的E2F蛋白表达水平下调,大剂量药物处理高MOI感染细胞2h和16h时E2F蛋白回复至正常细胞水平,其作用特点为:药物对高MOI感染细胞的拮抗作用优于对低MOI感染细胞的处理;大剂量药物作用优于中剂量药物;对病毒在感染早期(16h内)诱导效应的拮抗作用强于对病毒感染中期(24h)诱导效应的拮抗。

Results:AQP4 was expressed mainly in the normal gastric tissue basal side of parietal cell and principal cell and absent in carcinoma tissues.

结果:AQP4主要分布在正常胃底及胃体的壁细胞及主细胞的基底侧,细胞浆中表达较低,而在肿瘤细胞中表达则表达缺失?

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。