细胞的

Results 17β-HSD type 2 was found in the cytoplasm of tumor cells in 5.3% of cases.In adjacent non-malignant tissues,82.9% of cases were positive and the enzyme was detected in the cytoplasm of epithelial cells of the acini and ducts.

结果：2型17β-HSD在乳腺癌细胞的细胞浆中有表达，阳性率为5.3%；在癌旁非恶性乳腺组织腺泡上皮细胞以及导管上皮细胞的细胞浆中表达，阳性率为82.9%。

After maturation with the stimulation of LPS, apophyses became obviously increased by SEM scanning; the expression percentages of 33D1 and CD11c exceeded 80% by immunofluorescence staining and those of CD86 and MHCⅡwere 99.0% and 32.5%; MLR showed that mature BMDCs could better stimulate the proliferation of homologous T cells.

以LPS刺激成熟后，扫描电镜发现细胞突起更加明显，免疫荧光发现表达33D1和CD11c的细胞数均达到80%以上；流式细胞术检测CD86和MHCⅡ的细胞表达率分别为99.0%和32.5%；混合淋巴细胞反应提示成熟的BMDCs能明显刺激同源T细胞的增殖。

The myoblasts are the precursor of the maturated muscle cells, while the satellite cells are a kind of mononucleus cells which have the characteristics and function of the myoblasts. The satellite cells participate in the growth of skeletal muscle, the repair after injury of the muscle and provide nuclei to the maturated muscle for the normal alternation of cell nuclei. They are very important to the maintenance of the function of skeletal muscle.

成肌细胞为成熟肌细胞的前体细胞，而肌卫星细胞则是成熟骨骼肌组织中具有成肌细胞特征和功能的一类细胞，参与骨骼肌的生长、损伤后的修复以及为正常骨骼肌细胞核的更替提供细胞核，对骨骼肌正常功能状态的维持很重要。

The inhibition of SPG-Rg3 on the growth of MCF-7 cells was detected by MTT assay,IC50 was calculated to obtain the effective concentration; flow cytometry was used to observe the cell cycle of MCF-7 cells; AO/EB fluorescence double-dye technology was performed to observe the apoptosis of cells in morphology,immunocellularchemistry and RT-PCR were utilized to investigate the apoptosis of MCF-7 cells and its relationship with caspase-8 from protein level and molecule level.

采用MTT法观察SPG-Rg3对MCF-7细胞生长的抑制作用，并计算出IC50，依据IC50值确定SPG-Rg3的有效浓度；流式细胞术检测SPG-Rg3作用后MCF-7细胞周期的变化；AO/EB双染从形态学上观察SPG-Rg3对MCF-7细胞凋亡的作用；免疫细胞化学染色和RT-PCR技术分别从蛋白水平和分子水平上检测SPG-Rg3对MCF-7细胞的诱导凋亡作用及其与caspase-8基因的关系。

To further demonstrate the role of tumor cells in the induction of IL-17+Foxp3+ cells, the in vitro coculture system were performed.

为了进一步证实肿瘤细胞对於Th17细胞的产生有直接相关性，我们利用体外共同培养实验证实了的确肿瘤细胞能诱导IL-17+Foxp3+细胞的产生。

Methods With cell floating method, which is separating the cultured cell from coverslip and putting the cell in front of perfusion tubing, whole cell patch clamp technique was used to record hASIC1a currents evoked by low pH external solution.

使用全细胞膜片钳技术，将记录细胞与玻片分离，并使细胞位于输液管前，呈漂浮状态，以记录在低pH值外液中，HEK293细胞的酸敏感性1a型离子通道的电流。

Concludes:Three subsets of mast cell in human tissue are sorted and depurated with antibodies of specific enzyme and immunofluorescence labelling and flow cytometry. Mast cell of containing profuse secretary vacuoles could be displayed by confocal microscopy. It demonstrate that mast cell have material substructure offering for fast reaction of I type allergies of human being.

利用肥大细胞的特征性酶抗体、免疫荧光标记和流式细胞仪可将人组织中的肥大细胞分选纯化为三种亚型；以共聚焦显微镜显示肥大细胞含有丰富的分泌颗粒，它说明肥大细胞具备了为人体I型变态反应提供快速反应的物质基础。

Methods: After 48 h action of -300 V and -500 V electrets in 3T3 cells, CaCl2, A23187, EDTA were added in proper order to 3T3 cells. The levels of intracellular Ca2+ and the cell number of apoptosis were detected with flow cytometry.

选用-300 V和-500 V 驻极体作用3T3细胞48 h，即时依次加入CaCl2、A23187、EDTA，通过流式细胞术检测细胞内Ca2+浓度的变化和3T3细胞的凋亡量。

BMSCs could differentiate into CLCs in vitro induced by the supernatant liquid of the cardiomyocytes, 30% of which can make the induction effects reach the top.

心肌细胞的培养上清能够诱导BMSCs分化为心肌样细胞，且30%心肌细胞培养上清是诱导BMSCs向心肌样细胞分化的最适浓度。

Although someone has narrated cell framework of acellular membrane,no ideal preparation of ABCB or histomoiphometry of bone lacunas have been reported in details.

虽然，一些人对脱细胞的骨细胞外骨架进行了描述，但迄今为止还没有见到较理想的脱细胞骨细胞外基质的制备及骨细胞外基质骨陷窝计量检测的详细报道。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。