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GluLI was very weak at birth with very light staining of Purkinje cells and a very few granule cells.

二周时不光蒲氏细胞和它的树突,部分分子层细胞和GolgiⅡ细胞也有存在,颗粒细胞的阳性数还不多。

On day40 after infection,donor derived Thy-1.1~+ CD8Tm cells were detectable in various organs including peripheral blood,spleen,lymph nodes,and liver.These cells were CD44~,CD62L~/CD62L~ in phenotype and more importantly,these cells were readily detectable for intracellular IFN-γsecretion several hours after ex vivo restimulation with OVA_(257-264) peptide.

感染40天后,可以在包括外周血、脾脏、淋巴结和肝脏在内的脏器中检测到一定比例的Thy-1.1~+ CD8 T细胞,这群细胞具有记忆性CD8T细胞的表型特征,即CD44~,CD62L~/CD62L~,更重要的是,这群细胞在体外OVA_(257-264)肽再刺激数小时后即可检测到细胞内IFN-γ分泌。

The first split of microspore mother cell is observed after one week after beginning of temperature arised under high temperature but the split of secondary sporogenous cell doesn't be found. The split of secondary sporogenous cell is still found after one week but the first split of microspore mother cell is observed after 12d under low temperature. Formating speed of male gametophyte is affected by temperature.

在高温条件下,开始加温1周后即看到小孢子母细胞进行第一次分裂,未观察到次生造孢细胞的分裂;在低温条件下,1周后仍能看到次生造孢细胞的分裂,12d后才看到小孢子母细胞进行第一次分裂。

The result is consistent with previous studies. Whereas, tapetal cells of sterile anthers were disintegrated since tetrad cells were disjoined, indicating that disintegration of tapetal cells is earlier occurred in sterile anther than that in fertile anther. DNA ladder revealed that the cleavage of nuclear DNA into oligonucleosome-sized fragments to

结果表明,在花粉母细胞减数分裂后的四分体解离时期,不育花药的绒粘层细胞较可育花药提前开始裂解;在不育系S-Mo17rf3rf3花药和花粉S-(rf3)中均明显出现PCD过程的DNA片段化以及线粒体细胞色素C外渗的现象,证明了玉米CMS-S的花粉败育与花药绒粘层细胞的提前凋亡和小孢子细胞的细胞程序性死亡有关。

Kangdai Ⅰ has protective function to the damaged neurons and astrocytes: Main results:(1) It has direct protective function to the damaged neurons. It can increase the activity and survival rate, decrease the mortality and the transudation rate of LDH in cultured medium and the strong positive cell count of NOS expression of injured neurons.(2) It has also directly protective function to the damaged astrocytes. It can increase the activity and survival rate and protein content in conditioned medium.(3) It can strengthen the ability of BDNF, GDNF, bFGF, HSP and IL-6expression in damaged astrocytes.(4) It can also strengthen obviously the expressions of NSE, bFGF-receptor and bc1-2, lower the expression of bax and caspase-3.(5) It can indirectly protect and restore the damaged neurons by astrocytes. Because the effect of ACMK (ACM interfered by Kangdai Ⅰ) is stronger than ACM+K (ACM associated with Kangdai Ⅰ).

抗呆Ⅰ号对受损的神经元和星形胶质细胞均具有保护作用:主要表现为:(1)对受损神经元具有直接的保护作用,可提高受损神经元的活性和存活率,降低细胞培养液LDH的漏出率、细胞死亡率和NOS染色强阳性细胞的表达量;(2)对受损的星形胶质细胞也有直接的保护作用,可提高其活性、存活率以及培养液蛋白质的含量;(3)能增强受损星形胶质细胞分泌BDNF、GDNF、bFGF、HSP和IL-6的能力;(4)可明显增强受损神经元对NSE、bFGF的受体和bc1-2的表达,降低受损神经元对bax和caspase-3的表达;(5)抗呆Ⅰ号可通过星形胶质细胞间接地保护和修复受损的神经元,因为在多数实验组中经抗呆Ⅰ号作用的ACM的作用远大于ACM与抗呆Ⅰ号联合应用的作用,统计学上具有显著性差异。

Measure normal early pregnancy choria tissue and JAR cell by RT-PCR, LH/hCG receptor mRNA expression on both pregnancy choria tissue and JAR cell which is consistent with that be measured in ovarian granulosa cells, indicate that JAR cell and normal choria trophocyte presence LH/hCG receptor, hCG is one of the autocrine hormone.

二、用RT-PCR法可测到正常妊娠早孕绒毛组织、JAR细胞的LH/hCG受体mRNA表达,与正常卵巢颗粒细胞所表达的LH/hCG受体mRNA一致,提示JAR细胞与正常绒毛滋养细胞存在LH/hCG受体,hCG为其自分泌激素之一。

The morphology of tumor cells was observed and the change of quantity was assayed by trypan blue exclusion.

结果在 ACV作用下,GRC- 1细胞的形态及数量无明显改变;GRC- 1/ TK细胞被明显杀伤,数量明显减少,当含 2 5 。0 %GRC- 1/ TK细胞的混合培养细胞即可观察到明显杀伤作用。

To get more information of the function of human Era on cell cycle, the complete control inducible mammalian expression system was used to express human Era mutant and analyzed cell cycle of uninduced (add 1% ethanol) and induced (10μM Pon A) cell lines by flowcytometer, the primary results demonstrated human Era mutant could inhibit apoptosis induced by ethanol, and Era S36N may stimulate the expression of Bcl-2 as detected by Western Blot.

在构建稳定高表达蜕皮激素受体基因的细胞株3V3的基础上,转染突变型人Era的表达载体进行压力筛选,对所得到的单克隆细胞株进行诱导与未诱导条件下细胞周期的分析,结果表明未诱导加酒精的对照细胞可以引起细胞凋亡,而Era诱导表达后可以显著抑制凋亡细胞的数量,Western表明Era S36N可以诱导细胞Bcl-2表达,提示人Era可能与细胞凋亡相关。

Morphology of the EPCs was observed, and the growth curve of the EPCs was investigated. Surface antigens of the EPCs were analyzed by the flow-cytometer. The capability of intaking the acetylated low-density lipoprotein of the EPCs was detected using fluoresencent chemical method. The vasoformative capability and genetic stability of EPCs were cultured in matrigel, and examined by karyotype analysis.

观察培养细胞的形态,测定其生长动力学,采用流式细胞仪及免疫细胞化学方法检测其表型,应用细胞荧光化学法分析EPCs对乙酰化低密度脂蛋白(acetylated low-density lipoprotein, ac-LDL)的摄取功能,Matrigel上培养并检测其形成血管能力,染色体核型分析其遗传稳定性,裸鼠实验、软琼脂实验观察致瘤性。

However, higher concentration of CRP may increase the expression of protein and mRNA of inflammatory factor in endothelial cells; 2 Higher concentration of CRP may participate in the development of inflammatory process in endothelium by the mechanism that CRP induce cells apoptosis and activate signal pathway involved in cell proliferation. 3 CRP is the member of pentraxins family, in which two faces of pentamer form a special bi-phase structure: one face is ligand recognition phase; the other face is effector phase.

总结: 1CRP是一种保守蛋白,在正常生理水平下对内皮细胞没有明显的诱导炎症发生的作用,仅在高浓度条件下有可能诱导细胞炎症相关蛋白及mRNA的表达; 2高于生理水平的CRP对内皮细胞的致炎作用主要通过诱导细胞凋亡及激活细胞增殖相关蛋白信号通路的表达; 3CRP是一种双面五聚体蛋白,结构具有特殊的二相性,能够分别结合内皮细胞和E-LDL。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。