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Those proteins are the cell special proteins of neuron, glia, photoreceptor, bipolar cell, amacrine cell, horizontal cell and the ganglion cell of retina respectively.

并且可表达视网膜的终末分化细胞的标志,包括:感光细胞标志Rhodopsin、双极细胞标志PKC、无长突细胞标志Syntaxin、水平细胞和无长突细胞标志Calbindin和视网膜节细胞标志Thy-1。

Of the 89 patients, 59 had columnar metaplasia with goblet cells, which were further separated into low-density goblet cell and high-density goblet cell groups based on the percentage of crypts with goblet cells, and 30 patients had columnar metaplasia of the esophagus without goblet cells.

这89例中,59例含杯状细胞,我们依据粘膜隐窝杯状细胞的比例又进一步将这59例分成低密度杯状细胞组和高密度杯状细胞组,另外30例不含杯状细胞。

Results一. Culturing and identification of human conjunctiva cells1. morphous of conjunctiva cellsCells were harvested by tissue cultivation after digestion. Ponderosus cells adhered after 48 hours. Cells were shown in shape of round, ellipse and polygon. Cell body was loose and lucency with nucleus in center, cell membrane was also clearly seen. Cells were arranged in inlay and fuse in film after 12-14 days.2. Immunochemistry testNomogeneous immunochernical positive granules against CK13 were observed in endochylema of human conjunctiva cells.3. growth curve of conjunctival cellsSubcultured cells were aged and feeble after 5th passage.

结果一、正常人结膜细胞的培养与鉴定1、活体细胞的形态消化后组织块培养法:48小时后见大量上皮细胞贴壁,细胞不规则呈圆形、椭圆形、多边形,细胞体肥大透亮,细胞核位于中央,胞膜清楚,约12-14天后细胞呈镶嵌状排列,融合成膜状。2、免疫组化鉴定培养的人结膜上皮细胞CK13免疫组化染色见阳性颗粒均匀分布于胞浆中。3、培养结膜细胞生长曲线传代培养多数样本于P5代以后开始出现衰老征象。

The present study is to culture otocyst cells in fetal, cochlear sensory epithelial cells of neonatal and mature respectively in vitro with refinement of culture media and techniques. Immunocytochemistry and Bromodeoxyuridine labeling are used to detect properties and mitotic status of cultured cells. With the observations about proliferation and differentiation in cultured cells, we want to find the origin of regenerative cells and to understand how these progenitors proliferate and differentiate.

本研究分别对胎鼠听囊、新生鼠听觉上皮和成年鼠听觉上皮用自然培养方法进行体外培养,并应用免疫细胞化学方法,对培养细胞进行染色标记和观察,系统了解哺乳动物听觉细胞发生、发育过程,以及这些细胞在体外的增殖和分化过程,旨在了解哺乳动物听觉细胞听觉上皮感觉细胞的来源,发现细胞生长的前体细胞。

Results: the positive cells mostly included ependymal cells of myelocoele, glial cells, anterior horn motor neurons, and epithelial cells of spinal pia mater and their expressions were mainly located on cell membrane. interestingly, aqp4 distribution in glial cells and ependymal cells showed certain directivity, but the former was mainly in the membrane side contacting with capillary endothelial cells and the latter mostly in the basolateral membrane of ependymal cells.

结果: 免疫组织化学染色和原位杂交结果显示胶质细胞、前角运动神经元、中央管室管膜细胞、软脊膜上皮细胞均表达阳性,aqp4主要分布于细胞膜,其中,胶质细胞和中央管室管膜细胞表达存在极性,前者主要分布在与毛细血管内皮细胞接触的一侧,后者主要分布在中央管室管膜细胞的基底侧膜。

Spinous layer: the innermost prickle cells are polygon, and from the inside to outside, they become more and more flat, they are connected to granular cells with some space between, besides the spinous process, under normal condition, there is cell tissue fluid that could help the metabolism of cells in between.

棘层:棘细胞自里向外由多角形渐趋扁平,与颗粒细胞相连,各细胞间有一定空隙,除棘突外,在正常情况下,还含有细胞组织液,辅助细胞的新陈代谢。

When electric fusion method was used for nuclear transfer, the fusion rate (46. 0%), cleavage rate (53. 9%) and blastocyte development rate (10.9%) of adult ear fibroblasts were significantly lower than that of fetal fibroblasts (64. 5%, 70.1%, 21. 6% respectively), fetal skin cells (71. 5%, 70.8%, 22. 1% respectively) and ovary granulosa cells (88. 2%, 79. 1%, 25. 5% respectively). There was no significant difference among other donor cells in the cleavage and blastocyst development rate of resconstituted embryos.

当用电融合法进行核移植时,成体耳部成纤维细胞的融合率(46.0%),卵裂率(53.9%)和囊胚发育率(10.9%)均显著低于胎儿成纤维细胞(64.5%,70.1%和21.6%),胎儿皮肤细胞(71.5%,70.8%和22.1%),以及卵巢颗粒细胞(88.2%,79.1%和25.5%);另外三种细胞间的卵裂率,囊胚发育率无显著差异,但卵巢颗粒细胞的融合率显著高于胎儿成纤维细胞和胎儿皮肤细胞(88.2%vs 64.4%,71.5%,P<0.05)。

The height of bulliform cell groups reached 0.18 of leaves thickness, which was another important quantitative characteristic to achieve function of bulliform cells.

泡状细胞的周长面积比仅占普通表皮细胞的0.723,是泡状细胞功能实现的重要数量特征;(2)以细胞面积、周长、等效圆直径、每平方公厘细胞数、周长面积比,可以建立小麦叶片泡状细胞Fisher's线形判别函数,判别正确率达99%;(3)泡状细胞组高占叶厚的比例达到了0.18,是泡状细胞功能实现的又一重要数量特征;(4)相关分析表明,泡状细胞组长、泡状细胞组高、泡状细胞组面积均与泡状细胞距离叶缘的位置呈极显著负相关。

The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞,用100 TCID50柯萨奇B3病毒(CVB3)感染心肌细胞,建立病毒性心肌炎实验模型,然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞,观察心肌细胞的形态和搏动,用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白,四唑蓝比色法测定心肌细胞活性和病毒抑制率,流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

Gambogic acid suppresses the proliferation of Jurkat cells and Raji cells and arrested cell cycle of them at G0/G1 phase.

藤黄酸能抑制Jurkat细胞和Raji细胞增殖,使Jurkat细胞和Raji细胞的细胞周期阻滞于G0/G1期。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。