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To 7 days after transduction, cells were observed and recorded with fluorescence microscope, the expression levels of report gene EGFP in tumor cells were examined by using flow cytometry and Western blotting, the expression of report genes luciferase was analyzed with luminometer to obtain the relative light units.

用荧光显微镜观察EGFP阳性细胞,流式细胞仪检测EGFP阳性细胞的百分率和单个细胞平均荧光强度,Western印迹检测EGFP蛋白表达的变化以比较各组病毒转导效率。

The cytotoxic activity of spleen cells to EL9611 cells targets from the erythroleukemia-bearing mice were significantly higher than the Concanavalin A lymphoblast targets after the transplantation.

荷红白血病小鼠syn-BMT及免疫治疗后,长期生存的小鼠的脾细胞对 EL961靶细胞的细胞毒活力显著高于其对 CAB靶细胞的细胞毒活力(P=0.000)。

The weight and protein content of the microsomes from different evolutionary animal and tissue cells were first determined, and the weight of microsome varies obviously and the protein content of microsome in unit is almost the same.

本文还首次系统比较了不同组织细胞和不同进化程度动物细胞的微粒体获得率及蛋白含量,得到了不同细胞微粒体获得率明显不同、单位重量微粒体蛋白含量却基本相同的结果,表明不同细胞ER含量不同,而单位ER内蛋白含量基本相同。

By the test of clonal growth in soft agar medium , we compared the ability of cloning and proliferating among these three kinds of cells. The cell cycle was analyzed by FCM and morphosis of cells was observed under transmission eletron microscope.

通过细胞生长曲线,比较卵巢癌细胞系HO-8910与8910-p93、8910一pCDNA3.1(转染空载体ncDNA3.l)的增殖情况;通过软琼脂克隆形成试验比较这3种细胞的克隆形成能力和增殖能力;利用流式细胞仪检测这3种细胞的细胞周期的改变情况:利用透射电镜观察转染p93基因的细胞的超微结构变化;利用裸鼠移植瘤实验比较HO七与8910个93在动物体内生长状况。

He type of ossein in group B was mainly type Ⅰ collagen while that in group A type Ⅱ and it exhibited as a process of fiber formation in group C. The reason for why TGF-β〓 can promote ossification induced by BMP is that can promote the differentiation and proliferation of mesenchymal cells to supply more target cells for BMP, promote the differentiation and proliferation of mature osteocytes and chondrocytes, inhibit metalloproteinase to decrease the destruction of bone tissue and promote calcification of bone matrix.

GF-〓能促进BMP骨诱导作用主要因其能促进间充质细胞的分裂增殖为BMP提供丰富的靶细胞、促进成熟骨及软骨细胞的分裂增殖、促进骨细胞产生Ⅰ型胶原而抑制软骨细胞产生Ⅱ型胶原,抑制金属蛋白酶的活性以减少组织破坏、促进基质钙化等作用而使骨生成能力增加,提示它和BMP具有协同作用。

Its expression reach to peak in G1/S conversion, and in GO phase, differentiation or apolexis, the mRNA levels of MCM proteins declined to so little that can not be detected.

MCM蛋白在增殖细胞中表达水平高,在静止期细胞或分化好的细胞中不表达或水平很低,提示MCM蛋白可以作为增殖细胞的特异标志。

But there were no statistical differences in these factors between AGEs group and control ones after RAGE were interrupted by siRNA.Conclusion In this study model,the phenomena of increased cell cycle relative factors and inhibited cell proliferation might be one of the endogenous compensating mechanisms in keratinocytes treated with AGEs.This mechanism was correlated with RAGE signal pathway.

在上述体外模型下,表皮角质形成细胞的周期调控因子升高,而增殖却下降且凋亡增加,可能是AGEs干预后细胞内启动内源性代偿调控机制,且这种调控机制与其受体途径相关;AGEs也可能还通过以上细胞周期调控因子以外的其他途径或机制影响细胞生物学功能。

The materials were first fixed in different fixatives, embedded in paraffin, sectioned serially into 4fi thickness and stained with dyes of safranin and orange G, Heidenhain's iron haematoxylin combined with Mallory's connective tissue stain, buffered Magn-Lillie eosin methyl blue, Feulgen's reaction and methyl green-pyronin.

3凡有核仁外移的细胞,绝大部分为嫌色细胞,而在嗜色细胞中,尤其是在嗜酸性的细胞中极少见到这种现象,因此核仁外移和细胞的种类之间存在着一定的关系。

Attached spindle-shaped cells were detached and labeled with a series of antibodies against blood vessel endothelial-specific markers.

从健康成人外周血中分离内皮祖细胞接种於添加VEGF,bFGF,IGF-1,EGF的M199培养基中,观察细胞集落、梭形贴壁细胞的出现时间和特异性细胞标志表达情况。

Results The hela cells which transfected with HSV-TK and IL-12 gene was obviously changed in appearance after 24 hours which added GCV, but the normal control cells growthed prosperity. The survival rate of the hela cells which transfected with HSV-TK and IL-12 gene was fall-off with the plus of tile GCV. The killing effect of GCV for the hela cells transfected with HSV-TK and IL-12 gene was more strengthener than that for the normal cells.

结果 加入GCV后体外培养联合基因转染的Hela细胞,24h后细胞明显发生形态上改变,正常对照组细胞生长旺盛;随着GCV剂量的加大,转染联合基因的Hela细胞的存活率呈逐渐下降的趋势,GCV对转染细胞的杀伤作用较对正常细胞的杀伤作用明显增强。

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推荐网络例句

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。