细胞的

The hilling activing of CD3AK+ cells showed significantly higher to K562/VCR cells than to K562 cells, while there was no difference of cytotoxicity between K562 /HHT and K562 cells.

4CD3AK+细胞对K562/VCR细胞的杀伤活性强于对敏感细胞K562，其对K562/HHT细胞的杀伤活性与其对K562细胞的杀伤活性比较，差异无显著性。

METHODS Human IL 6 gene was reconstructed in retrovirus vector and transferred into incasing cells PA317 by lipofectamine mediated method. The clones of the cells transferred with hIL 6 were selected by G418. Targeted NIH3T3 cells were infected with the virus granules secreted from PA317 and also selected by G418. The insertion and expression of hIL 6 gene in NIH3T3 cells were analysed with Southern blot and Northern blot. RESULTS Human IL 6 retrovirus vector (pZIPIL-6) was successfully reconstructed.

利用重组载体构建技术将质粒pUCIL 6 cDNA的目的片段连接于逆转录病毒载体上，并以脂质体介导的方法将重组载体转染包装细胞PA317，以G418筛选克隆细胞，浓缩克隆细胞上清以制备重组病毒液，继之感染NIH3T3细胞后，进行Southern blot和Northern blot分析，检测目的基因在靶细胞的整合与转录水平。

In vivo, CXCR4-A549 and A549 cells were injected subcutaneously into nude mice(each group in 6).Four weeks later the mice were scarified and the tumors in situ and the lungs were taken out to be examined histologically.(3) Th cell cycle distribution was measured by flow cytometry to analyse the effect of SDF-1/CXCR4 in the cell proliferation and atoposis. The changes of the intracellular calcium concentration after SDF-1 activation were aslo measured by flow cytometry. Western blot was used to anlayse the phosphorylation of AKT and ERK, and the expression of MMP-2 and VEGF-C.

3为探讨SDF-1/CXCR4在肺癌侵袭和转移中的作用机制：①利用流式细胞仪检测CXCR4-A549与A549两组细胞的增殖和凋亡，分析上调CXCR4的表达后，对细胞生长周期的影响；②利用流式细胞仪检测两组细胞钙离子内流强度，分析SDF-1/CXCR4对钙离子通道活性的调控；③采用Western blot实验检测两组细胞PI3K/AKT、MAPK/ERK相关信号通路的活性，分析SDF-1/CXCR4可能激活的下游信号分子；④采用Western blot和RT-PCR检测两组细胞肿瘤转移相关基因VEGF-C、MMP-2的表达，探讨SDF-1/CXCR4与VEGF-C、MMP-2的相互作用关系。

In vivo treated sera from ATG-treated patients produced positive test results for T CDC and T/B FXM, while the B-cell CDC crossmatch remained negative.

来自于ATG治疗的病人血清测试发现，引起T细胞的补体依赖细胞毒性反应和 T/B的流式细胞计数阳性结果相关，而同时，B细胞补体依赖细胞毒性交叉配血保持阴性。

The -300V and -500V electrets were applied on 3T3 cells for 24 and 48h, respectively, then the surface charge density on 3T3 cells and the intracellular free Ca2+ concentration and the number of apoptosis were measured by means of cell electrophoresis and flow cytometry method, respectively.

利用Fluo—3/AM Ca2+指示剂，采用流式细胞术测定3T3细胞内钙离子浓度的变化和3T3细胞的凋亡量，探讨驻极体诱导3T3细胞凋亡与细胞表面电荷密度和游离钙浓度变化之间的关系。

CD34+ cells were harvested from G-CSF mobilized adult blood and umbilical cord blood and incubated onto fibronectin/gelatin-coated Petric dishes in the presence of recombinant human vascular endothelial cell growth factorand basic fibroblast growth factor.

毛宁为证明出生后血液循环中存在内皮细胞的前体细胞，从G-CSF动员的成人外周血及脐带血中分离CD34+细胞，将之接种于纤连蛋白与明胶铺板的培养皿上，培养体系中加入重组人内皮细胞生长因子和重组人碱性成纤维细胞生长因子。

In this essay, the culture methods of corneal epithelial cell and keratocytes have been studied. The method of rabbit corneal epithelial cells has been improved in order to solve the problem that the cells moved slowly and could not form monolayer when the traditional tissue nubbles cultivation was adopted.

本文对兔角膜上皮细胞和基质细胞的培养方法进行了研究，解决了传统角膜上皮细胞组织块贴壁培养时细胞迁出慢，不易形成单层的难题，建立了兔角膜上皮细胞原代培养的方法。

The results show that: 1 supplementation of protein to maturation media improves cumulus expansion in vitro compared to the protein-free control, but cumulus expansion is not necessarily related to oocyte nuclear maturation in pigs, and cumulus expansion is not the criterae for determination of nuclear maturation of pig oocytes, but only the exclusion of the first polar body; 2 exposure of pig COCs to hormone supplements for 23-24 hours improved cumulus expansion but had no significant effect on nuclear maturation compared to that for 46-48 hours; 3 under our research conditions, supplementation of different proteins into different maturation media has different effects on porcine oocyte nuclear maturation, but has no significant effect on subsequent embryonic development after IVF; 4 the nuclear maturation rates of pig oocytes matured in mTCM+pFF and mNCSU+pFF are superior than that in mNCSU+FCS; 5 different maturation media have no effect on pig oocyte cumulus expansion and subsequent embryonic development after IVF.

结果显示：(1)在成熟液中添加蛋白质可以加强卵丘细胞的扩散，但猪卵母细胞的核成熟与其周围的卵丘细胞扩展没有必然的联系，卵丘细胞扩散或成放射状不宜作为猪卵母细胞核成熟的标准，只有排出第一极体才能作为猪卵母细胞核成熟的标志；(2)在猪COCs的46-48小时成熟培养的后23-24小时阶段去除成熟液中的激素不但可以保证卵母细胞的核成熟率，而且可加强卵丘细胞的扩散；(3)在现有实验条件下，在mTCM和mNCSU中添加10％pFF与在mNCSU中添加10％FCS相比可获得较高的猪卵母细胞核成熟率；(4)在不同的成熟液中添加不同的蛋白质对猪卵母细胞核成熟率的影响效果不一样，但对体外受精后的早期胚胎发育影响不明显；(5)成熟液种类对猪卵母细胞的卵丘细胞扩散和体外受精后的早期胚胎发育无显著影响。

METHODS The inhibitory effect of nobiletin on proliferation of SMMC-7721 cells was assessed by MTT analysis and growth curve assay. Morphological changes under microscope were observed by Giemsa staining. Hepatic cancer H22 model in mice was performed through subcutaneous inoculation of Right-Sidedness Axilla of KM mice. Different dosages of nobiletin 125, 250, 500mgkg^(-1 were injected by intragastric gavage everyday.

不同浓度的川陈皮素作用于人肝癌细胞SMMC-7721细胞，用MTT法、细胞生长曲线实验研究其对SMMC-7721细胞的生长抑制作用，Giemsa染色观察细胞形态变化，初步研究其体外抑瘤作用；建立小鼠H22肝癌移植性实体瘤模型，研究川陈皮素对肝癌的体内抑制作用。

Objective: Comparative and extended research among the newly found cell, microglia cell and atypical cell in the rat organ of Corti after aminoglycoside intoxication; to further explore quality, origin, and the relationship between the newly found cell and the neural stem cell or nestin progenitor cell.

目的：对大鼠耳蜗药物损伤后出现的新细胞与小胶质细胞、非典型细胞进行比较和扩展研究，探讨新细胞的代表性及其起源、转归、以及与神经干细胞或nestin前体细胞的关系。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。