细胞的

Objective: To investigate the effects of sulindac on the growth and motility of ovarian cancer cell SKOV3 and its involved mechanism. Methods: In order to detect the effects of sulindac on cultured SKOV3, sulindac with different concentration and untreated control were set up. The changes in morphology of SKOV3 were observed by phase contrast microscopy. MTT colorimetric assay was used to study the effects of sulindac on SKOV3 cell growth. Motility of SKOV3 was determined by scarification assay.

目的：研究舒林酸对卵巢癌SKOV3细胞的生长和细胞运动的影响以及对基质金属蛋白酶2（MMP2）、环氧合酶-2(COX-2)表达的影响：方法：将不同浓度的舒林酸加入培养液中观察其对SKOV3细胞形态的影响，采用MTT法观察舒林酸对SKOV3细胞生长的影响，通过划痕试验观察舒林酸对SKOV3细胞运动的影响，利用免疫细胞化学方法研究舒林酸对SKOV3细胞MMP2、COX-2表达的影响。

Methods: in order to detect the effects of sulindac on cultured skov3, sulindac with different concentration and untreated control were set up. the changes in morphology of skov3 were observed by phase contrast microscopy. mtt colorimetric assay was used to study the effects of sulindac on skov3 cell growth. motility of skov3 was determined by scarification assay.

目的：研究舒林酸对卵巢癌skov3细胞的生长和细胞运动的影响以及对基质金属蛋白酶2（mmp2）、环氧合酶-2(cox-2)表达的影响：方法：将不同浓度的舒林酸加入培养液中观察其对skov3细胞形态的影响，采用mtt法观察舒林酸对skov3细胞生长的影响，通过划痕试验观察舒林酸对skov3细胞运动的影响，利用免疫细胞化学方法研究舒林酸对skov3细胞mmp2、cox-2表达的影响。

Nonspecific cytotoxic cell is the evolutionary precursor of mammalian natural killer cell in teleostean fish, which was mainly separated from blood and lymphoid organ as the first barrier of defense to protect the animal against bacteria, virus and protozoa parasitism, it plays an important role in nonspecific immunity and also has a function of killing tumor cells in vitro.

自然杀伤细胞进化上的前体细胞在鱼类中被称作非特异性细胞毒性细胞，主要来源于血液和淋巴器官，是防御细菌、病毒、寄生性原生动物等入侵的第一道防线，在非特异性免疫中起重要作用，还具有体外杀伤肿瘤细胞的功能。

Then we transfected transitorily the recombinant of green fluorescent protein gene and middle molecular weight neurofilament cDNA into wide type N2a (N2a/wt) and N2a/tau40 to observe the effect of tau accumulation on GFP-NFM fusion protein transport in cellular processes in living cells. At last we used an apoptotic inducer, camptothecin (an inhibitor of topoisomerase-1) to treat N2a/wt and N2a/tau40 cell lines, and compared their apoptotic response.

主要结果如下：一、tau蛋白过度表达和聚积对细胞形态的影响：倒置显微镜下观察两种细胞的形态，发现N2a/wt细胞的突起多而长，而N2a/tau40细胞胞体变圆，突起明显缩短；免疫印迹结果显示转染了tau40的细胞内tau的免疫反应约增加14倍，免疫荧光结果显示N2a/tau40细胞胞体内呈现出较强的红色荧光，tau主要分布在核周和突起起始部分的胞质内，而N2a/wt细胞内的荧光很弱。

According to our results, since BaP increased the expression of COX2 and pp38 to reduce the osteoblast differentiation and this phenomenon can reverse by treatment of NS398, but not by treatment of SB203580, suggesting that COX2 activation plays an important role on osteoblast differentiation. Adlay could increase osteoblast differentiation, in co-culture system, and reverse the osteoporotic status in mice, would be a helpful healthy food for enhance osteoblast differentiation. However, about, TBT, we didn't have enough data to support its effect on the differentiation of osteoblast or osteoclast.

所以依据我们所做出的实验结果，虽然苯骈芘会增加COX2和pp38的蛋白表现，但造骨细胞的分化作用仍下降，但若同时添加NS398，则分化能力有恢复现象，这或许可推测说COX2活性在抑制造骨细胞分化上扮演了一个重要角色；薏苡仁在共同细胞培养可以增加造骨细胞的分化作用，且对骨质疏松状态的小鼠具有缓解的能力，因此或许是能促进造骨细胞分化的营养食品；至於三丁基锡由於实验的数据不多，推测其或许并不影响造骨细胞和破骨细胞的分化作用。

Methods:The effect of chrysin on HIV1 infection and replication in vitro were detected by cell fusion of MT2 cells and HIV1ⅢB/H9 cells labeled calceinAM and the inhibition study of syncytia formation and p24 production monitored in culture supernatant of MT2 cells infected by HIV1ⅢB, respectively. Flurescence conjugated monoclonal antibodies and flow cytometry were used to detect the expression of CD69 by activated CD4+T in response to PHA.

以CR对CalceinAM标记的慢性感染HIV1ⅢB的H9细胞（H9/HIV1ⅢB）和MT2细胞的融合、对HIV1诱导的MT2细胞形成合胞体及对感染HIV1的MT2细胞产生p24抗原的影响，探讨其抗HIV1活性；以PHA刺激人外周血单个核细胞，利用流式细胞术，检测CD4+T细胞CD69表达的百分率，分析CR对免疫活化的影响。

Then the cell proliferation activity was detected using MTT colorimetrics, the cell cycle and apoptosis after LPS stimulation using flow cytometry, and expressions of caspase3, Bcl2, Bax using immunocytochemical staining.

mg/L的LPS作用于细胞，采用MTT染色法检测LPS对细胞增殖的影响；采用流式细胞术检测细胞周期及凋亡；采用免疫组织化学染色技术检测细胞的caspase3、Bcl2、Bax表达变化。

EGF remarkably stimulated the dermal sheath fibroblasts, dermal Tibroblasts and demal papilla cells. Effection on the dermal sheath fibroblasts was the strongest. bFGF, GM-CSF, insulin and hydrocortisone had not effective on them.

EGF对真皮鞘细胞、真皮成纤维细胞和毛乳头细胞有显著的促生长作用，其中对真皮鞘细胞的作用最强，而bFGF、GM-CSF、胰岛素+氢化可的松对三种细胞没有明显的作用。

Hyalinocytes can phagocytize the granulocytes which have expelled their granules. In hyalinocytes, ACP was found synthesized in Golgi apparatus and was involved in the endocellular digestion process.

透明细胞可以吞噬排放颗粒后的颗粒细胞，透明细胞中由高尔基体合成的酸性磷酸酶等溶酶体酶主要用于透明细胞的细胞内消化作用。

General characteristics of cells, its chemical constituents ( protein, nucleic acid, carbohydrate, lipid), cell structure , cell cycle, cell division, four kinds of tissues (epithelial, connective, muscular, and nervous), organ, ten organ systems (dermal, skeletal, muscular, digestive, respiratory, circulatory, excretory, endosecretory, nervous, and reproductive).

细胞的一般特征，细胞的化学组成(蛋白质，核酸，糖类，脂类)，细胞的结构，细胞周期，细胞分裂(无丝分裂，有丝分裂，减数分裂)，四大组织(上皮组织，结缔组织，肌肉组织，神经组织)，器官，十大系统(皮肤系统、骨骼系统、肌肉系统、消化系统、呼吸系统、循环系统、排泄系统、内分泌系统、神经系统和生殖系统)。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。