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Finally, because cnx-1 can further increase the germ cell death in ced-1 mutant, and the phenotype can be rescued by introducing cnx-1::gfp into the cnx-1; ced-1 double mutants, we suggest that cnx-1 acts in the engulfing cells other than the dying cells during the engulfment process of germline apoptosis.

除此之外,我们亦分析了cnx-1突变下ced-1(e1735)品种虫体之生殖母细胞的细胞死亡数量,发现cnx-1突变会额外地再提高ced-1突变下线虫生殖母细胞的死亡数量,且此现象可被外加入之cnx-1::gfp 所消除;这两现象显示出cnx-1在线虫生殖母细胞的细胞尸体吞噬过程亦具有功能。

Changdong" showed a noticeable enhancive effect when it was co-administrated with other anti-tumor agents or when it was co-applied with radiotherapy. The action mechanism study showed that an apparent cytotoxic effect of "Changdong" on ten tumor strains in vitro with a quantity-effect relationship was observed. Immunity experiment showed that "Changdong" could induce proliferation of spleen lymphocyte in mice bearing tumor and could improve NK cell" s activity, whereas has no impact on IL-2" s reproduction. It was also noticed that "Changdong" could induce human liver cancer QGY" s apoptosis in vitro, and it happened mainly on G0-G1 phase.

结果:"长动"对小鼠移植瘤模型和人体肿瘤裸鼠异种移植均有明显抑制作用,量效关系明显:"长动"与放疗和化疗分别联合应用有明显的增效作用:作用机制研究结果:细胞毒性试验研究表明"长动"在体外对10株肿瘤细胞有明显的细胞毒性作用,量效关系明显;免疫学试验表明"长动"可以诱导荷瘤小鼠的脾淋巴细胞增殖,增强NK细胞的活性,对IL-2的产生无明显影响;"长动"在体外可明显诱导人体肝癌QGY细胞凋亡,使细胞阻滞于G0-G1期。

Five models were used to investigate the "Changdong"" s anti-tumor effect: mice S180 sarcoma solid model, mice liver cancer H22 solid model, mice Lewis lung cancer model, as well as human liver cancer QGY and colon cancer LOVO implanted in naked mice; mice liver cancer H22 was also used to evaluate the enhancive effect of "Changdong" when it was co-applied with other anti-tumor agent or with radiotherapy; The "Changdong"" s cytotoxic effect in vitro on such tumors as QGY, LOVO, lung cancer A549, breast cancer MCF-7, as well as mice leukemia P388 was observed through MTT method. And investigate the effect of "Changdong" on proliferation of spleen lymphocyte, on activity of NK cell and on synthesis of IL-2 in mice bearing Lewis lung cancer, respectively. With propidium iodide straining, cell aigptosis and cell circle were analyzed by flow cytometry. Result:"Changdong" has an evident tumor inhibitive effect on mice tumor model as well as human tumor implanted in naked mice with a quantity-effect relationship.

使用小鼠S180肉瘤、小鼠肝癌H22实体瘤和小鼠Lewis肺癌模型,以及人体肝癌QGY和人体肠癌LOVO裸鼠异种移植模型,进行"长动"的抗肿瘤药效学研究:用小鼠肝癌H22进行"长动"合并放疗和化疗的增效作用研究;用MTT法进行"长动"对肝癌QGY、肠癌LOVO、肺癌A549、乳腺癌MCF-7、小鼠白血病P388等10株人体和动物肿瘤细胞的体外细胞毒性作用研究;用淋巴细胞转化试验、小鼠NK细胞活性试验、小鼠胸腺细胞增殖法分别测定"长动"对荷Lewis肺癌小鼠的脾淋巴细胞增殖的影响、自然杀伤细胞活性的影响和对小鼠产生白介素-2(IL-2)的影响;用流式细胞仪PI单染色法进行"长动"对人体肝癌QGY细胞的体外诱导凋亡作用和对肿瘤细胞周期的影响的研究。

Methods: Human colon cancer cell line DLD1 was treated with Bcl-XL siRNA, The protein level of Bcl-XL in the cells was determined by Western blot; the apoptotic ratio and survival condition were detected by flow cytometry assay and trypan blue-stained cell counting with hemocytometer, respectively. Results: The level of Bcl-XL protein in DLD1 cells was obviously down-regulated by Bcl-XL siRNA.

采用人结肠癌细胞株DLD1,利用人工合成的Bcl-XL靶向小分子干扰RNA转染DLD1细胞,通过Western blot法检测转染细胞Bcl-XL蛋白的表达,流式细胞仪检测处理后细胞的凋亡情况,锥虫蓝染色法计数存活细胞。

According to the specialty of the structure,it may be predicted that the function of Kail gene come down to cell-cell adherence and cell-matrix connection.

结构分析提示它在细胞与细胞间粘附、细胞与基质的连接中可能起有一定作用,研究也证实它可以增强肿瘤细胞之间的聚集能力,降低其吞噬能力及侵袭能力,而不影响细胞的增殖力。

Based on the detailed analysis of a variety of factors affecting stability of taxol production in Taxus cells, it was discovered that initial cell density and the relevant concentrations of substrates, elicitors and precursors significantly affected stability of taxol production; farther study showed that addition of elicitors at appropriate cellular state was the key to steadily improve taxol yield, especially, the proper cellular state was at the end of lag phase and at the beginning of the stationary phase, which was also proved by the cellular ultrastructure.

对影响红豆杉细胞紫杉醇产量稳定性的各类因素进行详细分析,发现细胞的接种密度以及与之相适应的培养基的基质浓度、诱导子浓度和前体浓度是影响紫杉醇产量稳定的重要因素;进一步研究发现,选择细胞处于合适的状态时加入诱导子是稳定提高紫杉醇产量的关键,细胞合适状态特别是延迟期末和稳定期初最合适,细胞状态的超微结构观察结果也证明了这一点;同时研究发现采用合适的同步化处理获得的细胞初始周期时相加入诱导子与紫杉醇产量稳定密切相关,且以低温同步化处理得到的细胞周期的分裂中期相的细胞加入诱导子不仅大大提高了紫杉醇的产量,而且诱导子加入时间大大缩短,有利于大大提高紫杉醇生产率。

The cells were passaged no more than 5 generations,and the cell freezing density is about 3×106 cell/ml, sample numbers of each kind are between 30 and 60, conserved numbers are 150-180 ampules,biological characters of fibroblast were analysed according to the request of constructing cell line. The results were as follows: 1 Cultured cells'morpha is typical fibroblast,which is fusiformate or unregular triangle, cells is radiant,flaming or rotative during growing .Qingyuan flesh chicken's cytoplasmic tubercle is much more and shorter in contrast with Zang chicken and Langshan chicken,that can be regarded as different character from others.

细胞冻存代数在5 代以内,冻存密度为3×106个细胞/ml,每个品种的样本含量在30~60 个之间,保存管数达150~180 支,根据建系要求,对成纤维细胞的生物学性状进行分析,所得结果如下: 1 培养细胞形态为典型的成纤维状,为梭形或不规则三角形,细胞在生长时呈放射状、火焰状或旋涡状走形,清远麻鸡的细胞形态与藏鸡和狼山鸡相比,胞质向外伸出的突起较多,且较短。

In this study, we utilized NCCmelan5 and NCCmelb4 melanoblast, two melanoblast cell lines which were previously used to investigate the treatment of vitiligo, to investigate the anti-apoptotic effects of LED (630nm), and ruled out others causes of repigmentation including proliferation, mitochondrial metabolism, melanin production, migration distances of MBs after irradiation, to formulate the theoretical basis LED's treatment of vitiligo.

在此研究中,我们使用研究白斑常用的NCCmelb4 and NCCmelan5两株黑色素母细胞珠来研究LED 光源对黑色素母细胞的抗凋亡的细胞保护功能,并且排除其对於细胞直接造成细胞增生,细胞移动,或黑色素增加的机转,希望为其治疗白斑的应用提供依据。

Use Merge Cells Wizard each time you need to place values from several cells to one cell or when you need to join several cells preserving the values of these cells.

使用合并细胞向导每次你需要的地方,价值从几细胞的一个细胞或当你需要加入几个细胞保存价值的,这些细胞。

Cell biology, which aims to study the cells, is one of the basic topics in life sciences, and it researches on the structure and function of cell and organelle from the cellular, sub-micron and molecular level.

其中,细胞生物学是生命科学的基础学科之一,它是以细胞为研究对象,从细胞的整体水平、亚显微水平以及分子水平等三个层次,研究细胞和细胞器的结构和功能。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。