细胞的

Methods Proliferation,cytotoxicity in vitro and expression of CD11 c of the cells cultured in the two different media were compared.To observe the tumor target cells killed by adˉherent natural killer cellsthrough electroscope.

分别用AIMV及完全培养基培养粘附NK细胞和非粘附NK细胞，比较细胞的增殖能力、杀伤肿瘤细胞的能力、表达粘附分子CD11 c 的能力，并通过电镜对A-NK细胞所杀伤的肿瘤细胞进行形态学观察。

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗；(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力；(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性；(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡；(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型，应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况，明确其靶向性；(6)将荷瘤裸鼠分3组进行放射免疫治疗，分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

The results indicated that the content of cytosolic CaM in cells treated with exogenous Ca2+ has increased indistinctively, while fluorescence intensity in cells treated with TFP decreased. So we believed that exogenous Ca2+ has little effect on the expression of CaM. High concentration of TFP can enter yeast cells and combine to CaM to make it inactive, which is the reason that TFP restrain the growth of yeast cells. 5mmol/L EGTA could completely arrest the cell proliferation of MFP7 after 28h, when the fluorescence intensity in cells wasobviously increased with flow cytometry and LSCM.

被较高浓度TFP处理过的MFP7胞内荧光强度则相对较弱，推测是因为TFP进入胞内与CaM结合从而使其失活，这也是高浓度TFP抑制酵母细胞增殖的原因。5mmol／L EGTA处理28h左右后，酵母细胞的生长被抑制在G1晚期，此时通过细胞流式法和在激光扫描共聚焦显微镜下观察到胞内荧光强度显著高于对照，说明CaM的表达水平在G1后期开始上升；回加10mmol／L Ca~(2+)处理24h后，细胞开始恢复生长，细胞流式法测定胞内荧光强度有所下降，表明多数细胞完成G1／S转换，进入生长对数期。

NGF had obvious cytotoxicity on these cell lines ,which shows to be dose-dependent, especially for MGC-803 and BEL-7404 ,their IC_(50) value at the 24~ hours are 49.9mg/L and 55.46mg/L ; Main features of apoptosis under fluorescent microscope include reduction in volume,nuclear chromatin condensation, intensification under jacinth fluorescence; The early apoptosis rate of pristine cells which detected by FCM increases with NGF dose increasing.

2NGF对4种人肿瘤细胞均有细胞毒性作用，且呈剂量依赖关系，尤其是对MGC-803和BEL-7404的抑制作用更强，作用24h的IC50值分别为49.9mg/L和55.46mg/L，荧光显微镜下可观察到MGC-803凋亡细胞的主要特点为：细胞体积缩小，染色质固缩，橘红色荧光增强；流式细胞仪检测到MGC-803细胞的早期凋亡率随NGF浓度的增高而增加，各浓度组与对照组的早期细胞凋亡率比较有明显差异(P＜0.01)。

Sheep embryo with high-purity,high-quality and high security features to extract the cells from the completion of the injection can be divided into three phases:-Using the most advanced chemical and biological equipment from sheep embryos to extract the liver activity of the protein and remove impurities,heat-sensitive eggs White matter and may result in allergic reactions of various types of risk factors-The adoption of advanced technology to extract the cells separated,thus the effectiveness of different cells,such as chest Gland cells,placental cells,liver cells and so on dozens of different types of-Using the most advanced technology to maintain the freeze-dried cells of live sheep-embryo for the first time the use of only afew hours to keep The cell activity,and now,with the continuous development of science and technology,after the extract ion of the cells have been able to keep to five years.

羊胚胎素具有高纯度、高品质和高安全性的特点，从细胞的提取到针剂的完成可分为三个阶段：-运用最先进的生化磁粉探伤仪，从小羊胚胎的肝脏中提取活性蛋白质，并去除杂质、热敏感蛋白质及可能导致过敏反应的各类危险因子-通过先进的技术对提取的活性细胞进行分离，从而得到具有不同功效的活性细胞，如胸腺细胞、胎盘细胞、肝脏细胞等几十种不同类型-采用目前最先进的冻干技术来保持细胞鲜活性，羊胚胎素在最初使用时只能保持几小时的细胞活性，而如今，随着科学技术的不断发展，提取后的细胞活性已经可以保持到5年。

And the repression of NO-induced cell death with Ppbi-1 overexpression in transgenic lines was apparent even at SNP concentrations up to 1mM.In addition work,we found that most of suspension cells treated with 0.5mM SNP can be stained by Sytox green,some PCD morphologic characteristics such as chromatin condensation and margination can be observed.DNA Ladder was also detected with these cells.As to the control and the cell treated with 0.5mMSNP+20μM Est,the above-mentioned characteristics can\'t be detected.

对0.5mM SNP和0.5mM SNP+20μM Est处理的飞廉转基因细胞培养三天后用特异性核染料sytox染色，0.5mMSNP单独处理的细胞大部分核被染色，而且细胞核出现了核凝聚、以及染色质边集等凋亡细胞的特征；提取细胞DNA电泳分析，观察到了DNA LADDER，这说明，该细胞可能已经发生凋亡，而Est诱导Ppbi-1基因表达的细胞均未出现这些凋亡特征。

The freezing technology of papanicolaou test is one meliorative technique based on papanicolaou test, the technique emphasizes on every process of sampling,smearing and staining, the most important step is the fixed smears should be soaked into the restoring-liquid of cells morphology based on the theory of penetration and expansion principle,thus achieve the purpose to cells restoring their normal appearance,to promoting cells staining and to improving cells transparency and clarity, it will be favorable to improve the cells recognition rate,the positive rate,the specification,sensitivity and decrease leaking rate and misdiagnosis rate.

冷冻式巴氏涂片技术是建立在传统巴氏涂片基础上的一种改良技术，该法在制片的过程中强调注重取材制片的全过程，其核心步骤是把固定好后的细胞玻片置入细胞形态修复液中，利用渗透膨胀的原理对已干枯萎的细胞进行修复，从而达到恢复细胞形态的目的，促进细胞着色，提高细胞透明度和清晰度，有利于对病变细胞的识别和提高对宫颈病变细胞的阳性检出率、特异度和灵敏度，降低漏诊误诊率。

PC12 cells were exposed to RA with different concentrations for 72 h and the lengh of neurite and max diameter of PC12 cells were observed at 24,48,and 72 h respectively. The expression of MAP2 was detected by immunocytochemistry after PC12 cells were exposed to RA for 72 h.

不同浓度的RA组均诱导PC12细胞72 h，在24 、48 和72 h分别观察细胞的突起长度和细胞最大直径的变化情况；在诱导72 h后利用免疫细胞化学技术，观察不同剂量RA作用后PC12细胞向MAP2阳性细胞分化的情况。

PC12 cells were exposed to NGF with different concentrations for 72 h and the lengh of neurite and max diameter of PC12 cells were observed at 24,48,and 72 h, respectively. The expression of MAP2 was detected by immunocytochemistry after PC12 cells were exposed to NGF for 72 h .

不同浓度的NGF组均诱导PC12细胞72 h，在24、48和72 h分别观察细胞的突起长度和细胞最大直径的变化情况；在诱导72 h后利用免疫细胞化学技术，观察不同剂量NGF作用后PC12细胞向MAP2阳性细胞分化的情况。

Using GSA-〓 lectin histochemistry, the first appearance of microglia occurred at E13, usually stained as scattered amoeboid cells. From then on, the number of microglia increased steadily, reached a peak at postnatal day 7 (P7), and then reduced gradually to adult level. Generally the morphological form of microglia undergoes transformation from early rounded or ameboid to adult resting ramified as they differentiate during development.

使用凝集素GSA-〓亲和组织化学染色，本实验最早观察到小胶质细胞在胚胎13天（E13），为散在分布的圆形或阿米巴样细胞，随后细胞数量逐渐增多；总体而言小胶质细胞的形态遵循着由圆形或阿米巴样逐渐转向纤细分枝状的演变规律，不同阶段细胞形态各有其特点，在不同区域也存在差异；小胶质细胞的数量在生后第七天（P7）达到高峰，其后开始逐步降低直至成年。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。