细胞的

Fibroblasts have the ability to secret many kinds of extracellular matrix and cytokines relevant to hepatogenic differentiation. So, we investigate whether coculture of hADSCs with fibroblasts without cell-cell contact play a role in hepatic differentiation of hADSCs by RT-PCR analysis of hepatic specific gene expression and real-time PCR analysis of Alb expression.

成纤维细胞能够分泌很多利于干细胞向肝细胞分化的细胞外基质和细胞因子，我们采用非接触共培养的方式对成纤维细胞与人脂肪干细胞共培养能否引发其向肝细胞的分化进行了研究。

In this study, Mono-nuclear cells containing hematopoietic stem/progenitor cells were isolated from umbilical cord blood of normal full-term deliveries, healthy adult and heterocellular HPFH patient bone marrow by density gradient centrifugation. After simple purification and expansion in vitro, MNCs were induced to erythroid cell differentiation using different culture systems and cytokines including Epo, IL-3 and GM-CSF.

本研究首先利用密度梯度离心方法，从正常成人和异细胞型遗传性胎儿血红蛋白持续存在综合症家系患者的骨髓组织、健康足月正常分娩胎儿脐带血中分离得到了含有造血干／祖细胞的单个核细胞(Mono-Nuclear Cell，MNC)，进行初步纯化及体外扩增后，组合使用Epo、IL-3、GM-CSF等细胞因子，将造血干／祖细胞向红系方向进行诱导培养。

The results indicated that there were only single mucosal mast cell sunset in rat mammary gland.They had various shapes.Most of mast cells were located in the interalveolar stroma and intralobular connective tissue.

结果 ：AB- S染色显示，大鼠乳腺只存在黏膜型肥大细胞；无论是泌乳期还是静止期，乳腺肥大细胞大多分布于腺泡间和小叶间结缔组织中，细胞的形态各异，但细胞数量在静止期和泌乳期有显著差异，泌乳期的乳腺肥大细胞数量明显减少(P 。0 1)。

The proliferation of SGC-7901 cell line was markedly inhibited by shRNA-GC-CⅢ（ P .05）. There was changes in cell morphology, such as cell volume reduction, karyopyknosis and chromatin conglomeration. FCM showed that GC-C inhibition caused the appearance of sub-G 0 /G 1 peak which increased from 5.47%（48h after transfection） to 5.63%（72h after transfection）.

转染GC-C shRNA真核表达载体后SGC-7901细胞的增殖能力受到抑制（ P 。05）；细胞形态发生改变，细胞体积缩小，核固缩，染色质浓缩聚集于核膜；流式细胞术检测显示G 1 峰前出现亚二倍体峰，转染48、72h后凋亡率分别为5.47%和5.63%（ P nbsp； 0.05）。

Result 1 Magnetic nanoparticles, magnetic nanoparticles modified with antisense oligodeoxynucleotide of human telomerase reverse transcriptase induced HL-60 tumor cells to apoptosis, we could see typical morphologic change of apoptosis cells: karyopyknosis, chromation"s condensing and aggregation in nuclear, forming crescent-shaped or annulus structures to lean on edge of cell nucleus"s membrane and posing apoptosis body by Atomic Force Microscope, Fluorescence microscope, transmission electron Microscope 2 There was a significant difference compared with control group(p.01), inhibition ratio had significant positive correlation with medication dosage and time ;during 0.8-8μM dosage amplitude, inhibition ratio accrescenced by dosages increasing. However, the inhibition ratio would decrease when dosage over 8-80μM.

结果 1 磁性纳米粒子、修饰有端粒酶反义寡核苷酸的磁性纳米粒子诱导HL-60细胞发生凋亡，原子力显微镜、光学显微镜、荧光显微镜和透射电镜下均观察到HL-60细胞呈现典型的凋亡细胞的形态变化：细胞核固缩，核内染色质浓缩、凝聚、形成新月形或环状结构紧靠在细胞核膜边缘，并形成凋亡小体。2 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子对HL-60肿瘤细胞的生长和增殖有明显的抑制作用，与对照组相比有显著性差异(p＜0.01)，在剂量为0.8-8μmol／L范围内，抑制率随剂量的增加而增加，当剂量超过8μmol／L时，抑制率反而下降；3 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子可增强p53基因的表达活性，引起DNA降解损伤，反向调节细胞周期活动，促使细胞从G0期进入G1期，抑制肿瘤细胞的生长。4 修饰有端粒酶反义寡聚脱氧核苷酸的量子点能通过内吞作用进入HL-60肿瘤细胞的细胞核，可以在细胞内进行定位和促进HL-60肿瘤细胞的凋亡。

It is concluded that CIK cells have high lytic activity against 7721 hepatocellular carcinoma cell line,which can be enhanced by co-culture with DC. DC-CIK cells are highly effective immune cells.

DC与CIK共培养细胞是一种增殖活性和细胞毒活性均高于CIK细胞的免疫活性细胞。

Nestin-positive cells were mainly in the gastric mesenchyma and the neck region of the glands. In mucosa of small intestine, Musashi-1-positive cells and TERT-positive cells were mainly located between cell positions 4 and 7. Oct-4-positive cells were diffusely distributed in the crypts.

小肠黏膜中Musashi-1和TERT阳性细胞主要在距隐窝底部4~7个细胞的位置，Oct-4阳性细胞散在分布于腺体内，Nestin阳性细胞均分布在小肠黏膜间质，无腺体内着色。

Nestin-positive cells were in the mesenchyma of small intestine, none of which in the crypts. In mucosa of descending colon, Musashi-1-positive cells were mainly located between cell positions 1 and 4, and TERT-positivc cells 1 and 5. Oct-4-positive cells were diffusely distributed in the crypts and the mesenchyma. Nestin-positive cells were all in the mesenchyma, none in the crypts.

降结肠黏膜中Musashi-1和TERT阳性细胞分别主要在距隐窝底部1~4个和1~5个细胞的位置，Oct-4阳性细胞散在分布在腺体内和黏膜间质，Nestin阳性细胞分布黏膜间质内，腺体内无表达。

That GER cells which are likely hair cell progenitors could be cultured in vitro and generate stereociliary bundle-like structure when forced to express Hath1 suggests that the misexpression of Hath1 probably can induce the predifferentiation of pure hair cell progenitors into hair cells in vitro.

GER细胞作为毛细胞前体细胞可以实现体外原代培养，在Hath1基因重表达情况下，部分细胞的表面可见不成熟的纤毛样结构，提示Hath1基因也许可以诱导离体培养下的纯的毛细胞前体细胞向毛细胞的初步分化。

In addition of cytomorphologic identification, immunolhistochemic technique of bone morphogenic protein-monoclonal antibody, alkaline Phosphatase staining and concentration assays, as well as osteoblasts reaction to PTH-cAMP were used, according to the biologic character of osteoblasts.

除了在细胞形态学上对其鉴定外，还根据成骨细胞的生物学特性，首次引用了骨形成蛋白单克隆抗体免疫细胞化学技术，以及碱性磷酸酶染色和含量测定，成骨细胞对甲状旁腺激素-cAMP反应等方法，验证了经酶消化分离培养的兔颅骨细胞具有成骨细胞的功能和特性。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。