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Methods: Virus proliferation assay, cell viability assay and Western blot were performed to assess the selective replication and cytolysis of CNHK500 in telomerase positive liver cancer cells Hep3B, HepGⅡ, SMMC7721 and in normal cells.

方法利用病毒增殖实验、细胞活力实验、蛋白印迹分析来检测增殖病毒CNHK500在端粒酶阳性的肝癌细胞株HepGⅡ、Hep3B、SMMC7721及正常细胞中选择性增殖和溶解细胞的特性。

The success of the new treatment, which uses a phage enzyme called lysin to kill S. pneumonia, suggests that the strategy could significantly reduce the incidence of acute otitis media in the United States.

这种利用一种称为细胞溶解素的噬菌体酶抑制肺炎链球菌的新治疗方法的成功,意味着这种方法可能会明显减少美国急性中耳炎的发生率。

Using cytohischemical staining methods, with the results of comparison the dynamics of proteins and polysaccharides in the anthe wall cells and colule cells of different developmental phases in female and male flowers, the anormogenesis of anther tapetum in the inflecting phase of from bisexual flower to unisexual flower was observed. In microspore developed phase, the tapetum functions of preserving and transportingmutritive material for microspore development and of secreting callosal enzyme for decompositing callosal cell lost no normal guadrant formed, of being abnormal meiose of pollen mother cells, and then, the stamen aborted selectively in female flowers and pistil in male flower.

利用细胞组织化学染色技术,对雌、雄花雄蕊花药壁细胞及花药内细胞发育过程中多糖及蛋白质动态进行了比较,实验中观察到雌花在从两性至单性花转变时期雄蕊绒毡层在整个发育过程中表现异常,在小孢子发育过程中未能起到贮藏、转运营养物质供小孢子发育及适时分泌胼胝质酶溶解胼胝质壁的功能,并且花粉母细胞减数分裂异常而未形成四分体结构,进而导致雌花雄蕊选择性败育,而雄花中雌蕊组织也发生了选择性败育过程。

The data shoewed that esculetin, resveratrol enhanced LPL expression up to about 1.2 ~3.5 fold compared with the control group. In addition to fibrates, however, no effect on LPL expression by curcumin was observed. We concluded that esculetin and resveratrol enhanced LPL expression on CHO-K1 cell. We hope that we can apply these drugs to mutant form LPL, furthermore apply theses drugs into clinical treatment.

利用药物处理有转染带有完整人类脂蛋白解脂酶启动子和脂蛋白解脂酶cDNA 序列(-1715 ~+1643)质体的CHO-K1细胞,从细胞培养液以及细胞溶解物中发现,除了curcumin之外所有药物都可以使得细胞培养液以及细胞溶解液内的脂蛋白解脂酶活性有1.2至3.5倍的上升,由此结果可知除了curcumin外,esculetin和白藜芦醇以及其他fibrate药物可降低三酸甘油脂血症。

Brane, such as synoviocytes, macrophages, osteoclasts and TNF-αvv acts on different kinds of cell in synovial memchondrocytes, which can produce metalloproteinase,collagenase, stromelysin and so on, further induce pannus formation, joint inflammation, bone erosion and cartilage degradation.

TNF-α可作用于RA关节组织中的滑膜细胞、巨噬细胞、软骨细胞和破骨细胞,使这些细胞活化,产生金属蛋白酶、胶原酶、基膜溶解酶等,导致局部炎症反应和血管翳形成,进一步引起软骨破坏和骨侵蚀。

A part of microbial cells would autolyzed to release the intercellular alkanedegrading enzymes when they were cultured on alkane. Thus alkane could be degraded and synthesized alkane derivative with surface activity extracellularly. This surface-active agent would improve alkane emulsifying, and those unautolyzed cells could utilize the emulsified alkane sequentially.

微生物细胞在烃类基质中培养时,为了适应其疏水性环境以更好地摄取烷烃,会通过一部分细胞的自溶解体而使得胞内烃类代谢酶等释放,这样烃类在胞外被代谢生成具有表面活性的烷烃衍生物,该表面活性产物将促进烃类的乳化,使得剩余的未自溶解体的细胞可以借助生物表面活性剂而摄取降解烃类。

The results showed that with the extension of AVM treatment time, the relative neuron survival rate and the mitochondria fumaric reductase activity decreased, the percentage of neuronal apoptosis increased, the mitochondria swelled, the mitochondrial structure was damaged, cristae were broken and dissolved and there were vacuoli in base.

结果显示,随着染毒时间的延长,细胞的相对存活率下降;线粒体琥珀酸脱氢酶活力降低;PI-/Rh123-细胞百分数增加;线粒体肿胀,结构不清晰,嵴断裂、溶解、基质中有片状空腔现象。

Firstly, the acute cytotoxicity of profenofos on FG-9307 cells was studied, it was found that the cell growth rate was markedly reduced by profenofos at all the concentrations tested; and that the fine structures of the cells were also altered by profenofos, as evidenced by dilation of nuclear membranes and mitochondria cristae, and presence of enlarged lysosomes with engulfed organelles and numerous vacuoles in the cytoplasm.

丙溴磷对FG-9307的急性细胞毒性研究结果发现:1)在所有实验浓度下,细胞的生长速度均受到抑制。2)细胞的超微结构被丙溴磷所改变,最明显的特征则是线粒体损伤严重,嵴肿胀,部分溶解破裂;核膜肿胀,有溶解;细胞内的电子密度高的溶酶体增多;内质网的膜溶解,核糖体从粗面内质网上脱落下来,游离于胞质中。

Plasmin system is a vital enzyme family that function is on thrombulysis and remodeling the extracullur matrix.

纤溶酶系统是人体内重要的酶系,功能在于溶解血栓和重塑细胞外基质。

As one of the most important osmoregulation actor, firstly,Proline plays a keyrole in the osmotic potential adjustment in plant cells. Secondly, Proline can preventNaCl from damaging the structure of biomacromolecule in cells. Thirdly, the highdissolubility and non-rejection capability of Proline can enlarge the solution volumnof the cell, so the concentration of salt will be diminished in cytoplasm and the saltstress will be relieved. Forthly, the accumulation of Proline could prevent thedehydrolysis from sap cavity in cytoplasm. Fifthly, the synthesis of plant chlorophyllwill be diminished under the salt stress, but the Proline can provide request ofchlorophyll synthesis.

脯氨酸作为渗透调节剂对植物体细胞渗透势的变化起重要作用:一,在盐胁迫下,脯氨酸在植物体内是一种重要的渗透调节剂,它是一些高等植物和绿藻抗盐和抗旱的主要渗透剂之一;二,脯氨酸可以保护细胞中的生物大分子的结构,使之不被NaCl破坏,并能维持其完整的水合范围;三,脯氨酸的高度溶解性以及其对植物各种酶活性不抑制性,可以扩大细胞的溶解容积,从而降低细胞质液中盐的浓度,减轻盐的胁迫作用;四,细胞质中积累的大量脯氨酸可以防止液泡对细胞质的脱水作用;五,在盐胁迫下,植物叶绿素的合成受到抑制,叶绿素的合成需要脯氨酸。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。