细胞溶解酶

Methods: Virus proliferation assay, cell viability assay and Western blot were performed to assess the selective replication and cytolysis of CNHK500 in telomerase positive liver cancer cells Hep3B, HepGⅡ, SMMC7721 and in normal cells.

方法利用病毒增殖实验、细胞活力实验、蛋白印迹分析来检测增殖病毒CNHK500在端粒酶阳性的肝癌细胞株HepGⅡ、Hep3B、SMMC7721及正常细胞中选择性增殖和溶解细胞的特性。

Using cytohischemical staining methods, with the results of comparison the dynamics of proteins and polysaccharides in the anthe wall cells and colule cells of different developmental phases in female and male flowers, the anormogenesis of anther tapetum in the inflecting phase of from bisexual flower to unisexual flower was observed. In microspore developed phase, the tapetum functions of preserving and transportingmutritive material for microspore development and of secreting callosal enzyme for decompositing callosal cell lost no normal guadrant formed, of being abnormal meiose of pollen mother cells, and then, the stamen aborted selectively in female flowers and pistil in male flower.

利用细胞组织化学染色技术，对雌、雄花雄蕊花药壁细胞及花药内细胞发育过程中多糖及蛋白质动态进行了比较，实验中观察到雌花在从两性至单性花转变时期雄蕊绒毡层在整个发育过程中表现异常，在小孢子发育过程中未能起到贮藏、转运营养物质供小孢子发育及适时分泌胼胝质酶溶解胼胝质壁的功能，并且花粉母细胞减数分裂异常而未形成四分体结构，进而导致雌花雄蕊选择性败育，而雄花中雌蕊组织也发生了选择性败育过程。

Results Microscopically, different degrees of hepatocytic degenerative changes and hyperplasic fibrous tissue and typical false lobule formation could be seen. Timm's staining result showed uneven distributed black granular deposits in the hepatocytes. No specific PAS staining was observed. Utrastructurally, the mitochondria were increased in volume and dramatically different in shape. The number of lysomes were increased.

结果 光镜下肝细胞表现为不同程度的退行性变，胶原纤维增生以及典型假小叶形成；Timm's染色阳性，发现不均匀分布黑色颗粒或团块样物质沉积；PAS染色则普遍缺乏特异性染色；超微结构显示线粒体形态多样，体积增大，溶酶体增多，粗面内质网管腔扩张，附着核糖体的脱颗粒，以及胞质水肿，质膜溶解。

The results showed that with the extension of AVM treatment time, the relative neuron survival rate and the mitochondria fumaric reductase activity decreased, the percentage of neuronal apoptosis increased, the mitochondria swelled, the mitochondrial structure was damaged, cristae were broken and dissolved and there were vacuoli in base.

结果显示，随着染毒时间的延长，细胞的相对存活率下降；线粒体琥珀酸脱氢酶活力降低；PI-/Rh123-细胞百分数增加；线粒体肿胀，结构不清晰，嵴断裂、溶解、基质中有片状空腔现象。

METHODS: The rhBMP-2,expressed in Escherichia coli, was washed by Triton X- 100 and further purified by DEAE chromatography.The inclusion bodies were resolved in 8 mol/L urea, and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one - step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP - 2 were tested by induction of the alkaline phosphatase activity in C2C12 cells.

在大肠杆菌中通过温度诱导表达重组人骨形成蛋白-2经过Triton X-100清洗之后，又通过DEAE离子交换层析纯化包涵体，包涵体在8 mol/L尿素变性溶解，在氧化-还原(还原型和氧化型谷胱甘肽)复性系统中，通过简单的稀释复性，通过肝素亲和层析一步纯化法纯化重组人骨形成蛋白-2，最后通过诱导C2C12细胞产生碱性磷酸酶检测重组人骨形成蛋白-2活性。

Plasmin system is a vital enzyme family that function is on thrombulysis and remodeling the extracullur matrix.

纤溶酶系统是人体内重要的酶系，功能在于溶解血栓和重塑细胞外基质。

As one of the most important osmoregulation actor, firstly,Proline plays a keyrole in the osmotic potential adjustment in plant cells. Secondly, Proline can preventNaCl from damaging the structure of biomacromolecule in cells. Thirdly, the highdissolubility and non-rejection capability of Proline can enlarge the solution volumnof the cell, so the concentration of salt will be diminished in cytoplasm and the saltstress will be relieved. Forthly, the accumulation of Proline could prevent thedehydrolysis from sap cavity in cytoplasm. Fifthly, the synthesis of plant chlorophyllwill be diminished under the salt stress, but the Proline can provide request ofchlorophyll synthesis.

脯氨酸作为渗透调节剂对植物体细胞渗透势的变化起重要作用：一，在盐胁迫下，脯氨酸在植物体内是一种重要的渗透调节剂，它是一些高等植物和绿藻抗盐和抗旱的主要渗透剂之一；二，脯氨酸可以保护细胞中的生物大分子的结构，使之不被NaCl破坏，并能维持其完整的水合范围；三，脯氨酸的高度溶解性以及其对植物各种酶活性不抑制性，可以扩大细胞的溶解容积，从而降低细胞质液中盐的浓度，减轻盐的胁迫作用；四，细胞质中积累的大量脯氨酸可以防止液泡对细胞质的脱水作用；五，在盐胁迫下，植物叶绿素的合成受到抑制，叶绿素的合成需要脯氨酸。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。