细胞浆移动
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Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.
结果 (1)ATP对U937细胞的增殖有按摩明显的阻抑作用,加药48h后增殖的抑制率可达43%以上;(2)ATP处理的U937细胞周期发生改变,G1期细胞数按摩明显增多,ATP作用24h时G1期前出现亚二倍体峰——凋亡峰,凋亡细胞数为3.4%,作用48h时凋亡细胞数增多为22.7%;(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜,逐渐向核膜外移动,进入胞浆内再移向质膜内外面,紧贴质膜外面再逐步脱离细胞体,进入细胞基质中,成为游离的凋亡小体。
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Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.
结果 (1)ATP对U937细胞的增殖有明显的阻抑作用,加药48h后增殖的抑制率可达43%以上;(2)ATP处理的U937细胞周期发生改变,G1期细胞数明显增多,ATP作用24h时G1期前出现亚二倍体峰——凋亡峰,凋亡细胞数为3.4%,作用48h时凋亡细胞数增多为22.7%;(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜,逐渐向核膜外移动,进入胞浆内再移向质膜内外面,紧贴质膜外面再逐步脱离细胞体,进入细胞基质中,成为游离的凋亡小体。
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The observation with histomorphology and phase contrast microscope: osteoclasts were large in size, multinucleus, foam-like cytoplasm with vacuole and pseudopodia, and movable. Acid Phosphatase reaction is positive.
经组织形态学、光镜和相差显微镜观察,破骨细胞具有胞体大,多核,胞浆泡沫状间有空泡,胞浆有伪足样突起,并可移动,酸性磷酸酶反应阳性。
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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.
从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。
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The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.
集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐
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The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.
血精的原因很,以良性病变为主。