细胞毒素的

By sequencing randomly-selected clones from the library and usingbioinformatics, 73 novel expressed sequence tags were obtained, in which therewere 24 full-length cDNAs, including cytolysin gene, neurotoxin genes, and greenfluorescence gene. Functional study on these novel genes is an ongoing task in ourlaboratory.

对文库克隆进行序列测定和生物信息学分析，已经获得了73个海葵新表达序列标签，有24个全长cDNA，其中包括海葵细胞毒素、海葵神经毒素、绿色荧光蛋白等具有开发应用价值的新基因。

Objective: To study the expression and purification of a fusion protein of ricin A chain and green fluorescent protein.

目的：表达蓖麻毒素A链与绿色荧光蛋白的融合蛋白，用於蓖麻毒素A链在细胞内的转运机理研究。

We also analyzed the processes of secretion of Ustiloxin A in culture medium and in false smut ball in the field by immuno-gold labeling.

通过免疫细胞化学标记，我们分析了稻曲病菌分泌毒素的行为过程，并且分析了田间稻曲球中的毒素的分泌情况。

The objective of this study is to picture the signal tranduction pathway of apoptosis induced by MC so as to further investigate the impact of MC on transcription, expression and regulation of apoptosis gene and the related biological effects; illustrate the toxicological mechanism of MC; as well as discover the new biomarkers invovled.

本研究工作的目的是通过功能基因组分析的技术平台，探讨微囊藻毒素诱导肝组织细胞凋亡的信号转导通路，以便在此基础上，进一步深入研究该毒素对凋亡功能基因在转录、表达、调控水平上的影响，及由此引发的生物学效应；揭示微囊藻毒素的凋亡毒理机制，同时发现新的生物标记物。

Mucous membrane protective screen is formed by the join between cell of alvine mucous membrane and cell, atrophy of cell of alvine mucous membrane, wither dies, cellular connective can send alvine path to connect flabbily appear a gender to increase, provide passageway; chemical protective screen for the bacterium inside bowel and toxin the digestive juice that shows epithelial excretive mucus and alvine memory are in bowel and digest enzymatic the chemistry that waits for material to reach its to develop.

黏膜屏障由肠黏膜细胞和细胞间的连接构成，肠黏膜细胞萎缩、凋亡、细胞连接的松弛可致肠道通透性增加，为肠内细菌及毒素提供通道；化学屏障指肠上皮细胞分泌的黏液及肠内存在的消化液和消化酶等物质及其发挥的化学功能。

In this paper, the perturbations of CTX-IX, which is a kind of cardiotoxin isolated from Naja naja atra, on artificial liposome, reconstituted system of dellpidated ATPase and phospholipld and mouse lymphacyte were investigated in order to understand the membrane toxlcology of CTX-IX in view of molecular dynamics of membrane, give clues for searching new toxic polypeptides that could kill the harmful cells and establish a example for the study of lipld-protein interaction and corresponding significance in membrane function.

本文研究了广东中华眼镜蛇毒中分离得到的心脏毒素CTX-IX和人工磷脂水化聚集体、ATP酶-磷脂重组体系和小鼠脾细胞之间的相互作用，试图从膜分子动力学角度理解心脏毒素的膜毒理，为杀伤细菌等有害细胞的毒性肽的寻找提供线索，在理论上亦为脂蛋白相互作用与膜结构功能的关系研究提供一个范例。

To obtain the tervalent fusion toxin gene, three toxin gene fragments from three species of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio mimicus were connected with the flexible linker using overlap extension PCR. The three toxin gene fragments respectively encode the mature proteins of the thermostable direct hemolysin of V. parahaemolyticus, the cytotoxin of V. vulnificus and the heat-labile hemolysin of V.

采用柔性Linker和重叠延伸PCR方法将三个毒素基因，即副溶血性弧菌的耐热型直接溶血素基因tdh、创伤弧菌的溶细胞毒素基因vvhA和拟态弧菌的热不稳定型溶血素基因vmhA的成熟蛋白基因片段进行串联，得到三联融合毒素基因tdh-vvhA-vmhA。

However, the site of translocation step is not clear.

蓖麻毒素进入细胞浆的过程被认为是毒素中毒的关键步骤。

GRPRs may be potential carriers for cytotoxins, immunotoxins or radioactive compounds.

这里GRPRs可以作为细胞毒素、免疫毒素或者有放射活性化合物的有效传递体。

Antinociception and VGSC-modulation of BmK IT2 and BmK AS—1. The results from nociceptive flexion reflex and plantar radiation heat showed: BmK IT2 (a depressant insect-selective neurotoixn) and BmK AS-1 (a new-type β-1ike neurotoxin) have significant antinociceptive effects on rat peripheral nerve system. The antinociceptive effects of BmK IT2 on the carrageenan-inflamed rats were significantly stronger than those on the normal rats. Whole-cell patch-clamping showed: Both TTX-S and TTX-R Na〓 currents induced from rat DRG neurons were significantly inhibited by BmK IT2 and BmK AS—1, and BmK IT2 was more sensitive to TTX-R than TTX-S component. All the results suggested: the peripheral antinociception of BmK IT2 and BmK AS—1 might be attributed to the inhibitory effects of them on the TTX-S and TTX-R Na〓 channel located on the peripheral ends of rat DRG neurons with small diameter.

中文题名特异性钠通道调制剂－长链蝎神经毒素的药理功能研究副题名外文题名 The study of pharmacological function of the specific modulators on voltage-gate Na+ channel-long chain scorpion neurotoxin 论文作者谭智勇导师吉永华研究员学科专业生理学研究领域\研究方向学位级别博士学位授予单位中国科学院上海生命科学研究院学位授予日期2002 论文页码总数93页关键词神经毒素毒理学电压门控钠通道东亚钳蝎钠通道调制剂馆藏号BSLW /2003 /R338 /22 本论文工作利用细胞和行为药理技术系统地研究了东亚钳蝎长链神经毒素的抗伤害性效应、对电压门控钠通道的电生理调制作用、改变胞内离子浓度的效应，并分析了相关的药理功能机制。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。