细胞毒素的

A double salt of platinous cyanide and another cyanide .Of, relating to, or producing a toxic effect on cells.

毒害细胞的，细胞毒素的毒害细胞的，有关细胞毒素的或造成对细胞毒害的

In this paper, the type of Fusarium toxins, biological toxicity and detoxification methods of Fusarium toxins are reviewed.

笔者对镰刀菌产毒素的类型，镰刀菌毒素对植物细胞、动物细胞的毒害以及脱毒方法作一综述。

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

Specifically, we will (1) perform electrophysiological study on the interaction of CTXs with cardiomyocytes stripped of specific GAGs,(2) study the binding of CTXs from spitting cobra venom with corneal tissue and examine the possible involvement of GAGs in the disruption of regular superstructure of collagen fibril and (3) examine the role of polyproline peptide sequence of P-type CTXs in the lytic action of CTXs. The results of this study will not only help delineate the mechanism of cobra CTXs, a long standing problem in the toxicity of cobra venom, but also suggest a new approach to develop CTXs into a potential drug for cancer.

本计划将进行：心脏细胞在去除醣胺基前后的电生理研究及比较了解心脏毒素与眼角膜的结合情形，并探讨醣胺基在维持眼角膜透明度所扮演的角色探讨P形心脏毒素中所含脯胺酸对细胞溶离能力的影响我们预期本研究将可对心脏毒素的作用机制有深入的了解，并寄望在未来能利用所了解的新作用机制，将心脏毒素发展成能控制癌症的新药方。

Analysing ustiloxins inhibiting plant cell mitosis The inhibition experiment of growth of garlic radicle showed that there were more cells in the telophase stage in the garlic radicle dealed with culture filtrate of Ustilaginodea virens than those dealed with H2O. The cell volume of the former was bigger than that of the latter. So it seemed that ustiloxins could inhibit the cell mitosis, but not in the cell elongation.

毒素对植物细胞分裂的抑制作用分析：用大蒜根尖做抑制试验，发现用稻曲病菌培养滤液处理过的大蒜根尖呈分裂末期的细胞数目明显多于相同条件浙江大学硕士学位论文下对照的数目，抑制率为69%，且细胞明显比对照的细胞大，说明毒素抑制根尖细胞的有丝分裂，但不能抑制细胞的伸长。

In this dissertation, firstly, the conditions of isolation and purification of specific toxin fractions produced by Exserohilum turcicum has been studied, and a high-efficient method has been established;secondly, the toxins were analysed by UV-Vis spectrophotometry;then the effect of specific toxin on death rate of corn leaves protoplast was studied by FDA as a tinct reagent;finally, using ANS as a probe and SDS-PAGE to study the membrane protein of the corn leaves with Htl gene. In a word, the aim of the research is to search some existent evidences of the toxin binding site on protoplasmic membrane, and provide proofs for nosogenesis of specific toxin in terms of cytology and the molecular biology.

本研究对玉米大斑病菌1号小种毒素的特异性组分进行分离，建立了高效的特异性毒素分离纯化方法，并对其进行了紫外波谱分析；用荧光素双醋酸酯染色法，测定了毒素特异性组分对原生质体死亡率的影响；进而用1-苯胺基-8-萘磺酸荧光探针标记法和SDS-PAGE电泳法对特异性毒素组分与Htl基因玉米细胞互作过程中的质膜蛋白进行了研究，旨在证明Htl基因玉米细胞质膜上存在特异性毒素组分结合蛋白，为从细胞和分子生物学角度阐明毒素的致病机理提供证据。

Anthraquinones are also reported to have antiprotozoal and cytotoxic activities.

蒽醌也被认为具有抗原生动物的和细胞毒素的行为特征。

The book comprehensively reviews the molecular and cellular biology of the most important microbial toxins with a focus on recent advances in the field, covering topics such as toxin ADP-ribosylation of proteins, toxin inhibition of protein synthesis, modulation of actin cytoskeleton, cytolethal distending toxins, cell-damaging/pore-forming toxins, clostridial neurotoxins, toxins of the immune system, streptococcal and staphylococcal toxins, anthrax toxins, Shiga toxin, Yersinia toxin, mycotoxins, and the regulation of toxin expression, as well as the potential of microbial toxins in bioterrorism.

该书全面评论了最重要的微生物毒素的分子与细胞生物学，重点是这一领域中的最新进展，主题包括蛋白质毒素ADP核糖基化，毒素抑制蛋白质合成，肌动蛋白细胞骨架的调控，细胞致死增加毒素，细胞损伤/孔状毒素，梭菌属的神经毒素，免疫系统的毒素，链球菌和葡萄球菌毒素，炭疽毒素，Shiga毒素，Yersinia毒素，毒枝菌素，和毒素表达调节，以及生物恐怖中微生物毒素的潜在可能性。

We may well explain why ricin-induced apoptosis showed no typical DNA ladder. The presence of laddering in DNA gels implies an apoptosis process, but its absence does not rule out the apoptosis. The ricin 0.05μmol〓 had no effect on the 〓 phase of cell cycle, but induced 〓 arrest. The ricin may affect the balance of synthesis and destruction of proteins that controlling the cell cycle.

检测到DNA梯度表明细胞发生了细胞凋亡，未检测到DNA梯度不能否认凋亡的存在。0.05μmol.L〓的蓖麻毒素对HeLa细胞周期的G〓/G〓期无影响，而诱导了G〓/M期阻滞，蓖麻毒素可能引起一些控制细胞周期蛋白的合成与降解发生紊乱。

The oxidase system includes both membrane-bound and soluble cytosolic proteins. The primary feature of this enzyme is stimulus-dependent activation and which mechanism is the phosphorylation and translocation of a complex of cytosolic factors to the membrane, where they associate with a flavocytochrome enzyme.The fully assembled complex functions as an electron transport system,moving electrons from cytosolic NADPH to molecular oxygen to form superoxide anion（O2-）, which, along with subsequent reactive products called respiratory burst,exerts microbicidal and cytotoxic activities.

此氧化酶体系包括膜结合的和可溶的胞浆蛋白，其主要特征为依赖刺激而活化，机制为复合体的胞浆因子磷酸化并转位至膜，在这里与细胞色素酶相关联，组装完全的复合体在功能上作为一个电子转运体系，从胞浆NADPH转移电子给分子氧形成超氧阴离子（O2-），伴随着接下来的活性物质产生，也叫呼吸爆发，发挥抗菌和抗细胞毒素的活性。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。