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We observed that a majority of dsRNA was localized in the nuclear periphery discontinuously after 24 hours of transfection. Time course showed that the labeled dsRNAs were maintained in cultured silkworm cells for up to 5 days. After six days, the signal was too weak to be detected.

用荧光显微镜观察FAM标记的dsRNA在细胞内的定位,发现在转染24小时后,dsRNA开始越来越多的聚集在细胞核的周围,并呈不连续分布,细胞内的荧光强度也越来越强。dsRNA在家蚕细胞中可以维持5天的时间,但到第6天时细胞内已检测不出荧光信号。

One view is that the nucleocapsids get tegument in the nucleus, and by budding into perinuclear space, they obtain their envelope from the inner lamella of the nuclear membra...

一种意见认为,核衣壳在细胞核内获得被膜,并通过出芽在内层核膜处获得其囊膜后,进入核周间隙,进一步经与核周间隙相连接的内质网,以囊泡转运的方式经高尔基体到达细胞膜,囊泡膜与细胞膜融合,病毒颗粒释放到细胞外。

There were nanobacteria in the nucleus and intracellar and extracellar endothelial cells under transmission electron microscopy after co-culture. The cell vacuolar degeneration, membrane structural necrosis, mitochondrin edema, mitochondrin vacuolar degeneration and lysosome myelinization were found in the endothelial cells.

混合培养后的内皮细胞透射电镜观察见细胞间、细胞内均有纳米细菌存在,细胞核内也可见纳米细菌,细胞空泡变,膜性结构类似坏死,部分线粒体水肿,空泡变,溶酶体呈现髓鞘图像。

Inhibition of prolyl hydroxylase activity or expression can be effected using competitive inhibitors such as dimethyloxalylglycine or siRNA leading to the stabilization of HIF and other beneficial effects.

DMOG是一种脯氨酰羟化酶抑制剂,能够抑制细胞内HIF-1α的降解,继而使细胞核内很多基因(如EPO、VEGF、Ang1和Glut-1等)的转录和表达增高,继而发挥其保护性作用。

Immuno-staining results showed the nuclear signals both in BEL-7402 and HCC tissues, while GFP subcell study also support the interesting distribution pattern.

通过免疫组化研究,发现在肝癌细胞BEL-7402以及肝细胞癌组织切片中,细胞核内均有阳性信号出现,而GFP的亚细胞定位也支持存在TIMP-1的核内分布现象。

The cochleae of animals were dissected. propidium iodide, a dna intercalating fluorescent probe, was used to trace the morphological changes in ohc nuclei. f-actin staining was used to determine missing ohcs. the single strand dna in apoptotic ohcs of guinea pigs and mice, and apoptosis inducing factor and endonuclease g in ohcs of guinea pigs were examined by immunofluorescence method.

分离解剖耳蜗后,用碘化丙啶染色细胞核、pholloidin染色f-actin,免疫荧光抗体分别染色单链dna、核酸内切酶g和凋亡诱导因子(apoptosis inducing factors, aif),制备耳蜗铺片,激光共聚焦显微镜下观察凋亡和坏死毛细胞内的荧光信号变化。

Positive staining of oxytocin and vasopressin were in the cytosol of the neurons and the former gave less intensity,many positive varicosities for vasopressin were also noticed in neurite.

催产素的免疫阳性产物主要在细胞核周围的胞浆即核周质内,而加压素的免疫阳性物质除了在核周质内有很强的反应外,在突起内也可见很强的免疫反应。

Moreover, andrographolide blocked nuclear translocation and DNA binding activity of NF-κB.

此外,穿心莲内酯也会减少 p65 进入细胞核内以及 NF-κB与 DNA 的结合。

The part of a bacterium or virus that contains nucleic acid and is analogous in function to the nucleus of a eucaryotic cell.

在每一个细胞内,都有一个像球一样的部分,这叫细胞核,它组织细胞内的一切活动。

Histologically, the seminiferous tubules contained numerous Sertoli cells and more Sertoli-spermatozoa complexes, accompanied by the depletion of Leydig cells with deeply stained nuclei. Mature spermatozoa were stored up in the epididymis, but only a few in the efferent ducts. In the second place was testicular atrophy(32/120; 26.7%). The seminiferous tubules showed moderate to severe inactivity of spermiogenesis with evidence of only spermatogonia, spermatocytes and Sertoli cells. The Leydig cells were obviously decreased in numbers associated with decrease of lipid droplets in their cytoplasms. Testicular hypoplasia was the third disorders(22/120; 18.3%). Only a few spermatogonia and Sertoli cells appeared without any spermiogenesis. The associated changes was decreased in Leydig cells and fibrous hyperplasia in the interstitium.Epididymal stones were sometimes found(12/120; 10%). Grossly, yellowish-white nodules with various sizes and firm in consistency were observed in the epididymis and the front efferent ducts. Microscopically, the epididymal ducts were dilated with voluminous spermatozoa storage, even showed calcification in severe cases. The deposited calcium salts were stained positively by Von Kossa and Alizarin red methods.Amyloidosis was also detected in 10 roosters(8.3%). Eosinophilic, homogeneous, amyloid-like substances were deposited mainly in the testicular interstitium and the periphery of blood vessels. These substances showed positive reaction by Congo red staining. Five roosters(4.2%)had Marek's lesions in the testis, epididymis and peripheral nerves with infiltration of pleomorphic lymphocytes. Only one case showed epithelial necrosis of seminiferous tubules accompanied by fibrous proliferation in the interstitium.

结果发现,在总共搜集的120个病例中,其中因年老所导致的产精力不佳为最多,占38例(31.7%),於镜下可见大量精虫黏附於Sertoli cell的表面,并可见Sertoli cell数量明显增多而Leydig cell明显减少,且其细胞核呈现浓染的现象,而在其副睪中仍可见到成熟精虫蓄留於管腔中,但在其输精管内却只有少量精虫存在;其次为睪丸萎缩,占32例(26.7%),镜下可见中度至重度无造精作用,其生精小管中只见到精母细胞、精原细胞及Sertoli cell存在,但Leydig cell数量明显减少且其细胞质内的脂质也明显减少;睪丸发育不全,占22例(18.3%),於生精小管内只见到精母细胞及少量Sertoli cell存在,不见造精细胞分化,於生精小管间质可见Leydig cell减少并伴随结缔组织增生;副睪结石,占12例(10%),肉眼下可在副睪及输精管前段见到黄白色大小不一的结节,触感坚硬,於镜下可见副睪管扩张并有大量成熟精虫蓄积,严重时可见钙化现象,以Von Kossa及茜素红染色均呈阳性反应;类淀粉沉著症,占10例(8.3%),镜下在睪丸间质及血管周围可见粉红均质样的物质沉积,以刚果红染色成阳性反应;马立克病,占5例(4.2%),镜下可在睪丸、副睪实质及周边神经内均可见到嗜碱性大小不一的淋巴样细胞浸润;睪丸坏死,占1例(0.8%),镜下可见生精小管上皮细胞坏死脱落及间质结缔组织增生。

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