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In the brain of adult rat, the positive immunohistochemical product of lSL-l (ISL-l-positive) was mainly located in the neuronal nucleus and found in discrete regions except to brain cortex, such as the Purkinje cell layer and the granular cell layer of cerebellum, the granular cell layer and the pyramidal cell layer of hippocampus, the mitral cell layer, the internal and external plexiform layer, the granular cell layer and the granular cell layer of olfactory bulb and so on, and several nuclei of the hypothalamus, midbrain and pons, such as claustrum, anterior olfactory nucleus, accumbens nucleus, caudate-ptamen, pallidum, substantia nigra, striatum, islands of Callaje, mammillary nucleus, anterior pretactal nucleus, habenular nucleus, amygdaloid nucleus, cuneate nucleus, rubral nucleus, gigantocellular reticular nucleus and so on.

在正常成年大鼠脑中,同源框基因islet-1表达产物(ISL-1)免疫组织化学阳性物质广泛分布于除大脑皮层外的神经细胞的细胞核内,ISL-1阳性神经元密集分布于小脑Purkinje细胞层和颗粒细胞层、海马的颗粒细胞层和锥体细胞层、嗅球的内丛层、外丛层、颗粒细胞层及僧帽细胞层等,另外在丘脑、中脑和桥脑的一些重要神经元核团均有分布,如,屏状核、前嗅核、伏核、尾壳核、苍白球、黑质、纹状体、Calleja岛、乳头体核、前顶盖前核、缰核、杏仁核、楔束核、红核网状巨细胞核等。

Results:The AgNOR results of malignant pleural effusion were obviously higher than those in tuberculosis pleural effusion(P.01).There were many dispersion,congregation and commix in the morphological study of AgNOR particles in malignant pleural effusion,but single form in tuberculosis pleural effusions.

结果:癌性积液组AgNOR的10项参数测定指标均与良性组有显著性差异(P.01),并且癌性胸腔积液组癌细胞核内AgNOR颗粒以弥散型、聚集型和混合型为主,而结核性积液组细胞核内AgNOR颗粒以单一型为主。

The distribution of ubiquitin positive dystrophic neurites and neuronal intranuclear inclusions were investigated. Results Morphologically,it was characterized by marked atrophy of bilateral striatum with loss of neurons and mild proliferation of astrocytes.

泛素阳性的营养不良性神经突起和神经细胞核内包涵体主要出现在大脑皮层的Ⅲ~Ⅴ层,神经细胞核内泛素阳性包涵体在大脑额叶前区皮层达22%,依次为顶叶7%、枕叶3%、颞叶1%和扣带回1%。

It turned out that many inclusions were found in the nucleolus and the cytoplasm of PK15 cell. The paracrystalline arrays was found in the inclusion in cytoplasm whose electron dense is deep; The nuclear of PK15 cell dissolved and there were margination of multiple chromatin aggregates and suspected intranuclear viral inclusion in PK15 cell.

细胞质中包涵体电子密度深,位于核膜周围,内有呈晶格状排列的病毒粒子,直径12±2nm;细胞核内染色质消散,在细胞核内发现多个电子密度深的、圆形或环状、由大量异染色质组成的包涵体,内有细微的粒状物质。

Thirdly,The nuclear chromatin of lung carcinoma cells concentrate to periphery, the electron density of cytoplasm increased, in some cells,the heterochromatin of nucleus were blurry,and the nucleole became uniform in electron density, the formatio reticularis of nucleole disappeared.

细胞核的改变:细胞核内染色质边集,基质电子密度增高。有些细胞核内异染色质分界不清,核仁呈均一电子密度,网状结构消失。

MNH6-GFP fusion protein was observed mainly in nucleolus. And this implied MNH6 protein mainly functions in nucleolus.

MNH6-GFP融合蛋白主要在细胞核内表达,说明MNH6蛋白的作用位点主要在细胞核内。

With DGD method, the electron microscopy observation has provided new information on the process of chromatin migration. That is: Firstly, the nucleus moved toward cell wall and a picture characteristic of synizesis stage of meiosis appeared; Secdonly, the transmigration of chromatin occured through CC, and a more extensive region constituted of nuclear skeleton left behind chromatin, which was named as"clear spaces" under light microscopy; Thirdly, at the late stage of synizesis, most of chromatin had gone into adjacent cells through CC and fused into a whole, and the nuclear skeleton left in the former cell gradually mixed with cytoskeleton in cytoplasm.

DGD—包埋去包埋电镜观察表明:百合花粉母细胞中有类似核纤层的结构存在;在染色质穿壁运动过程中,首先是细胞核向细胞壁靠拢,并可留下瞬间的运行轨迹——细胞核后方出现只有细胞骨架而少有细胞器的区域;其后是染色质开始穿壁,在穿壁染色质后方细胞核内出现无染色质仅有密集的核骨架的区域,到了染色质穿壁后期,大部分核物质都已穿至相邻细胞,并彼此融合,而残余在原穿出细胞中的核骨架己逐渐与原细胞的胞质骨架融为一体。

The Ca〓 in nuclear oscillated before GVBD and became stronger when Ca〓 transferred from extranuclear into the nuclear.

在GVBD发生前,细胞核内出现有规律的强度越来越大的波动现象,细胞核周围的Ca〓不断向细胞核内波动传递。

After treated with the samples, the MGC-803 cells are dyed with the fluorescence dye Hoechst 33258. Observed by the fluorescence microscope, the dense dyeing granulometric and nubby blue fluorescence can be seen in the nucleus of MGC-803 treated with AK-9 and AK-11, the nucleus of some cells smash to small fragments.

采用荧光染料Hoechst 33258对经初筛活性样品处理的MGC-803细胞染色,荧光显微镜观察,发现经AK-9及AK-11发酵液处理的细胞,其细胞核内可见浓染致密的颗粒或块状荧光,细胞核发生固缩、碎裂,而对照细胞的细胞核呈均匀弥散荧光,表明这2株菌株可能产生具有诱导细胞凋亡的活性物质。

A large number of necleocapsids were seen in the nuclei and cytoplasm at 12 h post infection, and the peak was at 24 h, but enveloped virions were rarely seen in the nuclei and cytoplasm.

结果表明:RNC在感染VZVJI6h后核内可见散在的核衣壳,12h后细胞核和细胞质内核衣壳明显增多,2 4h达高峰,而细胞核和细胞质内的成熟病毒颗粒较少见。

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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