细胞核内的

The transmition elec-microcope observation indicated that kangshuai Ⅰ prescription can chang the ultramicro fabric of nerve cell in quatar CA1 in hippocampus of the model rats by affecting the cell organs such as karyon、rough endoplasmic reticulum 、chondriosome、dictyosome and lipofuscin, which has similar effect with the original prescription.

透射电镜结果提示抗衰Ⅰ号各剂量组可以改善衰老模型大鼠海马CA1区细胞核、粗面内质网、线粒体、高尔基体、脂褐素体等细胞器的变化，尤以高剂量改善明显，与强力抗老液作用相仿。

Experimental methods: pathological slices: To observe the changes of pathomorphology and measure the circular area of internal elastic lamina , area of neonate intima and vascular lumen, the intimal proliferatory index and percentage, the index of lumen stenosis and positive index of proliferating cell nuclear antigen through the specimens stained by HE and immunohistochemistry.

实验方法：病理切片：进行HE染色和免疫组织化学染色，分别观察病理形态学改变；测定和计算受损血管内弹力膜环绕总面积、新生内膜面积、管腔面积、内膜增生指数、内膜增生百分比、管腔狭窄指数和增殖细胞核抗原阳性指数；观察MMP-2、MMP-3、TIMP-1和PDGF-BB的免疫组化表达。

Results The pathological changes in the nuclei mentioned above included the crimple and ruture of nucleus membrane,mitochondria degeneration and other degenerative structure such as plenty of primary lysosomes,agglomeration of lipochrome,Golgi complex degeneration and so on.

结果上述核团神经元的超微结构均发生病理性改变，神经细胞核膜皱缩，核膜凹凸不整，并有局部断裂；线粒体变性，基质浓度降低及空泡化；粗面内质网和高尔基复合体囊腔扩张变性；大量初级溶酶体及脂褐素集聚；出现了髓样体和多泡体等变性结构。

In result of electron microscope, cells of contrast group have large nuclear, plenty of euchromatin and microvillus, and organells, such as mitochondrion, Endoplasmic reticulum and Golgi apparatus are less.

电镜观察发现未经LTA作用的对照组细胞核大，常染色质丰富，细胞浆中线粒体、内质网、高尔基体等细胞器少，微绒毛较丰富。

"The union of nuclei within and arising from a single cell, as in certain protozoan s and fungi."

某些原生动物或真菌中发生于或在单个细胞内细胞核的接合作用。

Increase the bone marrow cell component while reduce the fat component, abnormal the caryon and cytomembrane structure of bone marrow cell and stromal cell, enrich the ribose body, mitochondrion and rough surfaced endoplasmic reticulum in cytoplasm of several organs and tissues, embody abvious improvement of heart muscle, liver, immnune organs, incretory organs and sexual gland.

增加免疫介导型再障小鼠的骨髓细胞成分，减少脂肪成分。使骨髓细胞和基质细胞的细胞核和细胞膜结构趋于正常，使多种脏器组织的细胞浆内核糖体丰富，线粒体及粗面内质网发达。对心、肝、免疫器官、内分泌器官及性腺等亦有明显的改善作用。

Cytolysosomes increased,cellular nucleus became twisted,rough endoplasmic reticulums swelled,and chondrosome became vacuolization.Dead intervertebral disk cells were located in ?nidi? which were made of multilayer degenerative collagen fibers.

术后8周实验组椎间盘开始出现大量死亡细胞，表现为溶酶体明显增多，细胞核扭曲，粗面内质网扩张，线粒体空泡化，死亡细胞外周的&巢&增厚呈多层排列，致密颗粒增多。

Results: The expression of death receptor 5 (DR5) was certificated in U343, DR5 appeared to be located in intracellular perinuclear compartment.

结果：神经胶质瘤细胞株U343表达死亡受体DR5，DR5分布于细胞内细胞核的周围。

The periostea of both experimental and control side of the mandibular ramus were taken and prepared, 2 of each 5 rabbits in a group were prepared for HE stain detection and 3 for proliferating cell nuclear antigen immunohistochemical detection.Results:1, The newly formed bone was detected on the lateral aspect of mandibular ramus after periosteal distraction. The bone was shaped like a hill. It looked very low and was full of holes at postoperative day 28. With the time of consolidation period lengthened, the newly formed bone matured gradually. X-ray examination showed the new bone shaped like a hill. The average values of new bone height at postoperative days 28,35,42 and 56 were 1.86 + 0.15mm, 2.29 + 0.29mm,3.19 + 0.13mm and 4.70 + 0.45mm. Histological examination of both HE stain and picricacid-fuchsin stain showed the increase in the number of osteoblasts and the change in the orientation of collagen fibers and bone trabecula. There were no significant differences between newly formed bone and original bone on the lateral aspect of mandibular ramus at postoperative day 56 histologically.2 Compared with the control side, the distracted periostea proliferated obviously under the microscope, and the number of periostealcells increased with satiation of cellular nuclear per unit area. The images of PCNA immunohistochemical stain of periosteum showed that the experimental periosteum proliferated obviously after distraction compared with the control side.

结果：骨膜牵张成骨的实验研究南京医科大学硕{学位论文l、骨膜牵张后，可见下领升支外侧的骨皮质上有新骨形成，新骨呈山峰状凸起，术后第28天的新生骨较低平，多孔隙，随着固定时间的延长，新骨逐渐成熟；下领升支前后向切线位X线投照显示新骨呈山峰样隆起；经测量，术后第28、35、42和56天组平均新生骨厚度分别为x.86士0.15mm、2.29士0.29mm、3.19士0.13mm和4.70 土0.45mm；脱钙骨组织的HE染色和不脱钙骨组织的苦味酸一品红染色的组织学观察均显示了新生骨在成骨细胞数量上的增长，以及胶原纤维和骨小梁排列方向上的变化，术后第56天的新生骨在组织学上与原升支骨组织已无明显区别。2、HE染色显示，与对照组相比较，实验侧骨膜增生明显，细胞间排列紧密，单位面积内骨膜细胞数增多，细胞核饱满；骨膜PCNA 免疫组化染色显示，与对照侧相比较，实验侧骨膜在牵张后出现了明显的增生迹象，PCNA阳性细胞分布紧密，单位面积内阳性细胞数较对照组多，靠近骨表面的骨膜中的阳性细胞数更多而且分布更为紧密。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。