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In addition, the role of the RER in protein body forming, the role of amyloplast envelope in amyloplast proliferating, the relations between nucleus and cytoplasm of starchy endosperm cells in rice, and the variation of nucleus in plant PCD, the roles of plasmalemma invagination, ER, Ca〓 and phosphatases during endosperm development were discussed.

本文对粗面内质网在蛋白体形成中的作用,淀粉质体被膜在淀粉质体增殖中的作用,淀粉胚乳细胞的核质关系,植物PCD中细胞核的变化,以及质膜内陷、内质网、Ca〓和磷酸水解酶在胚乳发育中的作用等进行了讨论。

In conclusion, we detailedly investigated the variation of membrane system (mainly plasmalemma, amyloplast envelope, ER) with the potassium permanganate fixative method, nucleus and organelles with routinely fixative method, and their functions with cytochemical method (localization of phosphatases and Ca〓).

综上所述,本文运用高锰酸钾固定法研究了膜系统(主要指质膜、淀粉质体被膜、内质网)的变化,运用常规固定制样研究了细胞核和细胞器的变化,采用细胞化学方法(Ca〓和磷酸水解酶的定位)探索了细胞器和细胞核的功能。

Calcium antimonite deposits, which indicates calcium distribution, were mainly localized in cellmembrane and vacuoles, as a storeroom of Ca2+, and smaller amounts of calcium p recip itates randomly resided in mitochon2dria, cytop lasm and nucleus.

结果表明,对照生长条件下(14 mmol·L-1 NO3-),黄瓜根系分生区细胞内Ca2+主要出现在细胞膜和小液泡内,线粒体、细胞质和细胞核及核仁内也有少量的较小颗粒钙沉淀。

After the nucleus of the sperm has united with the nucleus of the egg, the result of their union, the zygote, begins a process of internal division.

受精以后数小时内,也就是精子的细胞核与卵子的细胞核融合以后,所形成的这个受精卵开始内部的分裂过程。

The ultrastructure characters of pollen grain in Brassica napus are:1There are vacuoles in cytoplasm but no starch grain in uniceelluar pollengrain.2The veget- ative nucleus of bicellular pollen grain is generally spheroidal;while the generative cell is spinde-shaped,with a big nucleus,thin layer of cytoplasmand few cell orgens,no cell wall.3In three-celled pollen grain the sperm cell is separated from the cytoplasm of vegetative cell by two layers of plasmic membrances.

Brassica napus的花粉粒,在不同发育时期超微结构的特征如下:1单胞花粉粒有一个球形的细胞核和明显的核仁,细胞质内出现液泡,缺少典型的淀粉粒。2双胞花粉粒的营养核多为球形。生殖细胞纺锤形,无细胞壁,细胞核比例大,细胞质呈薄层,细胞器少。3三胞花粉粒有一个裂片状的营养核和两个纺锤形的精细胞,精细胞无壁,以两层质膜与营养细胞的细胞质相隔。

The location and structures of sex-pheromone-producing gland in female H.insularis were studied by EAG,GC,SEM,and TEM.These studies showed that thegland situate in the intersegmental membrane between the eighth and ninthabdominal segments,and is an eversible abdominal fold;Many plump cones disturbon the surface of the gland.The glandular cells of 2-day old virgin female H.insularis are arranged in one layer,among which the central cells are columnarepithelial cells and flat on two sides.The nucleus is irregular elliptical.There isevident conjugation between cells and the involution is more in the basal membraneof cell.Microvilli are distributed on the cytoplasmic membrane and linked withendocuticle on which there are many layers of chitin,and the outer cuticule is staineddeeper.The cell contains bubbles,mitochondria,glycogen deposits,roughendoplasmic reticulum and smooth endoplasmic reticulum.

结合触角电位、毛细管气相色谱、扫描电镜、透视电镜等技术对小线角木蠹蛾雌蛾腹尖末端不同组织部位提取物的测定分析以及腺体位置和形态结构的观察发现:小线角木蠹蛾性信息素分泌腺位于腹部末端8~9节之间,是一个由节间膜特化而成的上皮结构,为一可外翻的腹褶,腺体表面分布着饱满的锥形体,羽化后2天未交尾的雌蛾腺体细胞呈单层排列,腹面中央由密集的柱形上皮细胞组成,细胞排列向两侧延伸至背部,其形状由柱形逐渐变为扁平形,细胞核为椭圆形,细胞与细胞间有明显的胞连接,细胞基底膜基褶较多,质膜上分布着微绒毛,并与内表皮连接,内表皮上有多层几丁质,外角质层染色较深,细胞质中含有空泡、线粒体、脂质粒、粗面内质网和光面内质网。

The sex pheromone-producing gland of Ancylis.sativa Liu is amodified intersegmental membrane as a dorsal bag between eighth and ninthabdominal segments.

对枣粘虫雌蛾性信息素腺体的扫描和透射电镜观察表明,枣粘虫性信息素腺体是由第八、九腹节间的节间膜特化而成,是位于背部的一个囊状结构,它由两部分构成,前部的方形囊状体和后部的三角形囊状体,二者之间有一突起的脊;雌蛾静止时,腺体随第八和第九腹节一起嵌缩于第七腹节内,求偶时,腹部末端外伸,腺体细胞表皮外露,释放性信息素;腺体细胞呈方形,其表面覆盖几丁质表皮,表皮顶端无孔;细胞核很大,呈椭圆形,由双层核膜包被,位于细胞中下部,内有丰富的染色质;细胞质内有丰富的内质网,其内还有大量的大小不等的脂肪滴,以及线粒体和溶酶体等细胞器。

The injured gill cells had a different ultrastructure, with endoplasmic reticulum dilated and vesiculated, mitochondrial cristaes partially disintegrated, gill fibre cuticles denatured and dropped. The ultrastructural damages to hepatopancreatic cells were characterized by the decrease of microvilli, swelling and breaking up of mitochondria, expansion of endoplasmic reticulum, disruption and vacuolization of nucleus, tumefaction of nuclear membrane and increase of lipid droplets. As for the toxicological changes of myocardial cells, the mitochondria were swelling, having a lot of vacuolus and disintegrating, while the myofibrils were irregular with endoplasmic reticulum dissolved.

鳃细胞的损害表现为鳃丝水肿,细胞器溶解,角质层变薄,线粒体、内质网的肿胀、解体;肝胰脏细胞的主要损害特征为肝管微绒毛减少,线粒体水肿解体,内质网扩张,细胞核空泡化,核膜水肿和脂肪滴增加;心脏细胞的毒理变化为线粒体内嵴肿胀、瓦解,肌原纤维不规则,内质网溶解。

The cell in acidic situation presented a degenerative chang-ment,such as an increased lipid drop and empty cavity in the cell plasma,a py-knotic nuclei,the dilatation of the endoplasmic reticulum and Golgi saccules,mi-tochondrial swelling the number of ribose decrease,and the ribose dissociated inthe plasma,a lot of swallowing cavity,the fibric material decreased accompany-ing with the acid being stronger.

随培养基pH逐渐下降,上述过程因受酸性阻抑而延缓,细胞增殖速度也受影响,处于酸性环境中的软骨细胞呈退行性变化,表现为细胞浆内脂质颗粒与小泡增多、细胞核皱缩、核仁浓染、内质网线粒体膨胀、核糖体数目减少,且与粗面内质网结合的少,游离在胞浆中的多。

To study the effects on the body of animals after incorporation of 3H-TdR and its mechanism, the laws of distribution A retention and absorbed dose in blood , thymus, spleen, femur, heart, lung,kidney, liver, brain , spermary , small intestine and muscle were detected by liquid scintillation counter after 3H-TdR was injected into the tail vein of mouse; the process of dynamic incorporation of in the lymphocytic cell nucleus of blood , thymus , spleen and the nucleus of femur was monitored; the absorbed dose in the cell nucleus was estimated; the influence to RNA"s synthesizing and cell"s survival was observed using the technology of CLSM; A segment of Egr-1 gene was cloned with reverse transcriptase- polymerase chained reaction, the influence to the expression of Egr-1 gene in the spleen cells was observed.

为了探讨有机结合氚内污染对生物机体的影响及其作用机理,本课题应用均相液体闪烁测量技术研究了氚标记胸腺嘧啶核苷(~3H-TdR)经尾静脉注入小鼠机体后,在血液、脑、胸腺、心脏、肺脏、肝脏、脾脏、肾脏、小肠、睾丸、肌肉和股骨共12种组织器官的生物动力学分布规律及其吸收剂量;应用纸片法液体闪烁测量技术研究了~3H-TdR内污染在外周血、胸腺和脾脏淋巴细胞及骨髓细胞的动态滞留规律,估算了细胞核的吸收剂量;应用共聚焦显微镜观察了~3H-TdR内污染对脾细胞核酸合成及其存活率的作用,应用反转录PCR技术研究了~3H-TdR内污染对脾细胞早期生长反应基因-1(Egr-1)转录表达的影响。

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