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细胞核

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Results The endothelial cell and fibroblast of valves and vessel walls in experimental group were completely decellularized, no cell fragments were retained within the matrix scaffold; collagen fiber and elastin fiber had been preserved with intact structure and wavily arrayed; deoxyribonucleic acid content of valves and vessel walls in experimental group were decreased by 97.58%, 97.25% compared with that of control group.

结果 实验组瓣膜及血管壁中内皮细胞、成纤维细胞去除完全,无细胞核碎片;瓣膜及血管壁胶原纤维和弹力纤维呈波浪状排列、整齐,结构完整;瓣膜、血管壁的基因组脱氧核糖核酸含量分别较对照组下降97.58%和97.25%。

We first found out the preparation of intact budding yeast nuclei suitable for substrates for replication in Xenopus egg extracts.

我们首先摸索出了一个制备适用于爪蟾卵复制底物的完整芽殖酵母细胞核的方法。

TGF-β familyis one type of morphogen. Once binding with the receptors, they transfer the signal into thenucleus through Smads, and regulated the transcription of target genes.

TGF-β家族成员就是一种重要的形态发生因子,它们与靶细胞的受体结合后,通过Smad家族蛋白将信号传递到细胞核里,调节靶基因的转录。

In the dark, COP1 accumulates in the nucleus and represses photomorphogenic development, and the light diminishes the abundance of COP1 and abrogates its activity.

当植物在暗环境下生长时,COP1蛋白聚集在细胞核内,抑制光形态的建成,而在光环境下,COP1蛋白则分散到细胞质中,解除其抑制作用,恢复光形态建成。

At day 20, some of the acini collapsed, and the epithelial cells of the duct were damaged.

由10μg·L-1 B\P处理20d时,在鳃回血管中发现血细胞坏死后残留的固缩细胞核,鳃丝的上皮细胞排列不规则甚至断裂,部分消化腺泡崩解,鳃丝结构和消化导管上皮结构损害严重;消化盲囊组织结构的损伤比鳃更为严重。

The mice were immediately anatomized and the volume and weight of tumors were determined. Immunohistochemistry was used to determine the expression of PCNA protein and nm23-H1 protein of these cells.

接种6周后脱臼处死全部裸鼠,测量移植瘤的体积和重量,采用免疫组织化学法检测各组中增殖细胞核抗原和nm23-H1蛋白的表达。

The cochleae of animals were dissected. propidium iodide, a dna intercalating fluorescent probe, was used to trace the morphological changes in ohc nuclei. f-actin staining was used to determine missing ohcs. the single strand dna in apoptotic ohcs of guinea pigs and mice, and apoptosis inducing factor and endonuclease g in ohcs of guinea pigs were examined by immunofluorescence method.

分离解剖耳蜗后,用碘化丙啶染色细胞核、pholloidin染色f-actin,免疫荧光抗体分别染色单链dna、核酸内切酶g和凋亡诱导因子(apoptosis inducing factors, aif),制备耳蜗铺片,激光共聚焦显微镜下观察凋亡和坏死毛细胞内的荧光信号变化。

Five hours after the noise exposure, the cochleae were harvested. mitochondria energetic function, succinate dehydrogenase activity, in the hair cells of cochleae was evaluated with a colorimetric assay using blue tetrazolium monosodium salt. the sdh-labeled organs of corti were double stained with propidium iodide, a dna intercalating fluorescent probe used to visualize the morphologic viability of hair cell nuclei.

采用琥珀酸脱氢酶(succinate dehydrogenase,sdh)染色法进行耳蜗基底膜细胞线粒体染色,细胞核dna荧光染料碘化丙啶(propidium iodide,pi)双重染色耳蜗基底膜,以未受噪声暴露动物为对照,显微镜下观察噪声暴露后耳蜗基底膜核固缩和核肿胀毛细胞琥珀酸脱氢酶染色的变化。

Under fluorescence microscope, cells treated with curcumin showed shrunk or distorted nucleus with fluorescence conglomerated.

姜黄素作用后,Hoechst染色显示A549/DDP细胞核缩小变形,致密浓染荧光成团块分布。

The structure inside the cells of trains A2 and A5 changed clearly in different cultural conditions. PHB(poly β hydioxybutyric acid) was convolved in A2 and this material has poteintial value in the future.

经JEOL 1 0 0CX透射电镜观察,A2、A5菌在不同培养条件下细胞内部结构发生了明显改变。A2菌细胞核中含有的聚β羟基丁酸,具有巨大的应用价值和开发潜力

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