细胞核

Nuclear translocation of protein PDX-1 in different groups: Indirect immunofluorescence showed PDX-1 was mainly located in cytosolic slightly in the basical station, while translocated to nucleoplasmic greatly followed by stimulated with 16.7mM glucose.

为量化这种核转位程度，每组计数100个细胞并分别记数有荧光信号在核内表达的细胞数N及荧光信号在核周围表达的细胞核数C，比较各组的N／C值。

Fig.7 Western blot analysis indicated NuMA in nuclear matrix proteins of EC1 and EC18 cells.

图7 EC1、EC18血液细胞核基质蛋白中NuMA免疫印迹分析。1。

Yantai Normal College,264025,ChinaABSTRACT Most epithelial nuclei of Nuttallia olivacea are elliptic in shape and the chromatin is filiform.Most nuclei of connective tissue are round and have Feulgen positive granules.

摘 要 紫彩血蛤的鳃上皮细胞核多为椭圆形，核内染色质呈丝状；结缔组织细胞的核多为圆形，部分核内有块状Feulgen阳性颗粒。

Results There was significant negative correlation between the Nogo-A expression and the morphological change of tumor tissues according to immunohistochemistry. Western-blot analysis also indicated that the gray value of Nogo-A protein band in the oligodendroglioma group was significantly higher than that in the anaplastic oligodendroglioma group.

结果 免疫组织化学结果显示，Nogo-A免疫阳性染色的光密度值与肿瘤细胞核分裂、核异型比例呈明显的负相关，对蛋白印迹试验结果进行光密度分析也发现少突胶质细胞瘤中的Nogo-A蛋白条带灰度值明显高于间变型少突胶质细胞瘤中的灰度值。

Methods Thirty-nine patients with oligospermia were included in the trail. Cell blocks of seminal fluid were made and were observed under light microscope. Expression of PCNA and P16, incidence of apoptosis in spermatogenic cells were detected by immunohistochemistry and in situ 3'DNA end labeling technique.

39例原因不明少精子症患者留取精液，制备细胞块切片，采用免疫组化技术观察生精细胞增殖细胞核抗原及P16的表达；采用原位3'羟基末端标记法观察生精细胞凋亡。15例正常精液作为对照。

The results are as follows:(1) immunoreactive neurons of NPY in medulla oblongata of Taihe silky fowl, New jianghan domestic chicken are mainly localized in the inferior olivary nucleus.

结果如下：(1)在泰和乌鸡和江汉鸡的延髓中，NPY阳性神经元主要存在于下橄榄核，网状核及中缝核也少量出现；(2)在泰和乌鸡和肉鸽的小脑中，NPY阳性神经元主要存在于小脑皮质的蒲肯野氏细胞层，且以小叶顶端的蒲肯野氏细胞阳性明显，而小脑皮质的分子层、颗粒层未见阳性反应细胞；(3)泰和乌鸡延髓的网状细胞核非常发达；泰和乌鸡小脑皮质分3层，由外至内依次为分子层、蒲肯野氏细胞层和颗粒层；小脑分10叶，Ⅰ、Ⅱ和Ⅹ为单叶，其余各叶都分为2～3个小叶；(4)非洲鸵鸟迷走神经运动背核和疑核特别发达，孤束核吻侧部不发达。

Methods After ZTO injection acted on human ovarian cancer SKOV3 cells, the changes in the nucleus were observed by fluorescence microscope, oncosis index was counted by projection electron microscope, the situation of cell DNA breakage was observed by agarose gel electrophoresis, and cell Bcl-2 gene expression was observed by immunohistochemical method.

以不同浓度莪术油注射液作用人卵巢癌SKOV3细胞后，通过荧光显微镜观察细胞核变化、透射电镜计算细胞胀亡指数、琼脂糖凝胶电泳观察细胞DNA断裂情况、免疫组化观察细胞Bcl-2基因的表达。

This data suggested that the retardation of MK endoreduplication during ontogenesis might be related to intrinsic modification of MK progenitor cells.

该结果提示胎儿巨核细胞核内复制的延迟可能与其祖细胞的内在修饰第四军医大学硕士论文论文摘要特征有关。

As the maturation of nucleus and cytoplasm, oolemma is also matured progressively.

同细胞核与细胞质成熟一样，细胞膜也存在成熟问题。

In conclusion, these results suggested that (1) the ageing-associated decline in fertility of female KM mouse was due to a decrease in both quantity and quality of oocytes;(2) the ooplasm from young mice did not correct the meiotic errors of aged mice, and the ooplasm from aged mice did not induce abnormal segregation of meiotic chromosome of young mice, indicating that meiotic anomalies found in the oocytes of aged mice might be related to nucleus or chromosomes and relevant factors;(3) a critical nucleocytoplasmic ratio was essential for normal maturation and segregation of meiotic chromosomes of oocytes and development to 2-cell embryos.

本研究结果表明：（1）昆明白小鼠与衰老相关的生育力下降是其卵母细胞数量减少和质量下降的综合结果；（2）年轻小鼠卵母细胞细胞质不能纠正老龄小鼠GV的减数分裂错误，老龄小鼠卵母细胞细胞质也不诱导年轻小鼠的GV发生减数分裂错误，老龄小鼠卵母细胞减数分裂异常很可能与细胞核或染色体及其相关因素有关；（3）关键的核质比对卵母细胞正常的成熟、减数分裂染色体分离及2-细胞期胚的发育是绝对必需的。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。