细胞核

A digitalized study on the morphology and spatial organization of the nuclei in the neurogenetic SVZ of adult mammalian brain is accomplished in the present study, providing a basis for further investigation in this field.

此外，本研究实现了成年哺乳动物脑内神经生发区SVZ细胞核形态及其空间位置的数字化，可作为进一步研究SVZ神经生发的基础。

Cytolysosomes increased,cellular nucleus became twisted,rough endoplasmic reticulums swelled,and chondrosome became vacuolization.Dead intervertebral disk cells were located in ?nidi? which were made of multilayer degenerative collagen fibers.

术后8周实验组椎间盘开始出现大量死亡细胞，表现为溶酶体明显增多，细胞核扭曲，粗面内质网扩张，线粒体空泡化，死亡细胞外周的&巢&增厚呈多层排列，致密颗粒增多。

In the past twelve years there have been hundreds of studies showing these nonthermal effects--- such as DNA damage and nonrepair, opening of the blood-brain barrier (allowing toxins and pathogens to reach the brain), lowered immunity, decreased melatonin levels, effects on stress proteins, formation of micronuclei (aberrations in cell nuclei which are often markers for cancer), changes in calcium metabolism affecting communication between cells, changes in brainwave patterns as seen on EEG's, plus effects observed on many different systems of the body.

在过去十二个月里，已有数以百计的研究表明这些非热效应---如DNA损伤和nonrepair ，开放血脑屏障（允许毒素和病原体到达大脑），降低免疫力，降低褪黑激素水平，对应激蛋白，形成的微（畸变细胞核往往是癌症的标志），改变钙代谢的影响细胞之间的沟通，改变脑电波模式脑电图上看到的，加上效果观察许多不同的系统的机构。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

The appearance of DNA ladder from the ovules in which pollen chamber formation was undergoing, together with TUNEL assay and ultrastructural changes, demonstrated that the nucellar cell death, doomed to bring about pollen chamber, was a process of programmed cell death .

在贮粉室的形态发生过程中，相关珠心细胞的DNA电泳出现DNA ladder、其细胞核被TUNEL反应所标记以及它们的超微结构变化都说明，参与贮粉室形成的珠心细胞的死亡是一种细胞程序性死亡（programmed cell death，PCD）。

It was observed by means of electronic microscope that toxinresulted in the apparent change in ultrastructure of rapeseed leafincluding the damage of chloroplast membrane,dilated,disorderedgrana lamellae;the damage of mitochondria membrance andeventually vacuolated mitochondria;the contract of cell nuclein.

电镜观察发现：菌核菌产生的毒素引起油菜叶片超显微结构显著变化，主要包括叶绿体膜破坏，基质片层肿胀，排列紊乱，形成空腔，最终叶绿体解体；线粒体膜破坏，最后空泡化；细胞核质凝缩。

Actin has been identified in nuclear skeleton, nucleolar matrix and chromosome scaffold of these plants.

结果显示，三种植物细胞核骨架、核仁骨架和染色体骨架上存在肌动蛋白。

There were occasional enlarged hyperchromatic nuclei with one or more prominent nucleoli.

偶尔也可见到增大深染的具有一个或多个显著核仁的细胞核。

The nuclei of the Paget's cells are atypical and, though not seen here, often have prominent nucleoli.

Paget氏细胞的细胞核有异型性而且常有显而易见的核仁。

Through a time correlation analysis of spontaneous intensity fluctuations,this technique in conjunction with EGFP as a probe is capable of determining nucleoplasmic viscosity in terms of Stokes-Einstein equation as well as its corresponding analysis of the diffusion coefficient for EGFP in the nucleus.

研究中利用FCS技术测定绿荧光蛋白分子在细胞核内的扩散系数，并且根据Stokes-Einstein关系式计算出核浆粘滞系数，同时实现了对特定生理条件下核浆粘滞系数的跟踪测量。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。