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(1) In our research,frequent expression of BSAP in H/RS cell of classical Hodgkins disease provides further evidence for its B-cell origin,and the expression of BSAP on H/RS cells also can be in favor of the identification of H/RS cells and can distinguish HL from ALCL.(2) Expression of BSAP is located in nucleoli of cells,while that of CD20 in membrane.

结果 (1)33例cHL中,30/33(90.91%)H/RS细胞和33/33(100%)背景淋巴细胞BSAP表达阳性,而H/RS细胞10/33(30.30%)CD20阳性,1/33(3.03%)CD45RO阳性,31/33(93.94%)CD30阳性,25/33(75.75%)CD15阳性;(2)33例HL石蜡刮片组织中有16例(48.48%)出现IgH基因重排阳性,显微切割的19管H/RS细胞有14管(73.68%)扩增阳性,而12管背景淋巴细胞有2管(16.67%)扩增阳性;(3)测序分析周新华第一军医大学病理学教研室获得了一段免疫球蛋白重链可变区的重排片断;(4)原位PCR结果发现杂交的阳性颗粒不仅出现在探针来源的同一病例也出现在不同病例的HillS细胞核上,同时在部分周围背景淋巴细胞核上也见到阳性颗粒。

YC2 can increase the expression of GR in the liver cell nucleus, and improve the hyperfunction of hypothalamicpituitaryadrenal axis.

本实验中阴虚大鼠模型血浆CORT含量明显升高,肝组织细胞核内GR表达明显减少,而YC2能提高阴虚大鼠肝组织细胞核内GR表达,改善下丘脑垂体肾上腺轴功能亢进状态,减轻激素副作用。

The YC2 can increase the expression of GR in the liver cell nucleus, and improve the hyperfunction of hypothalamicpituitaryadrenal axis.

本实验中阴虚大鼠模型血浆CORT含量明显升高,肝组织细胞核内GR表达明显减少,而YC2能提高阴虚大鼠肝组织细胞核内GR表达,改善下丘脑垂体肾上腺轴功能亢进状态,减轻激素副作用。

After the nucleus of the sperm has united with the nucleus of the egg, the result of their union, the zygote, begins a process of internal division.

受精以后数小时内,也就是精子的细胞核与卵子的细胞核融合以后,所形成的这个受精卵开始内部的分裂过程。

We also have observed some organs pathological changes of juvenile Jian Carp, such as pancreas acinous gland and zymogen granules of acinous gland cell disappearing, intercellular substance hyperplasia and inflammatory cells soakage, hepatatrophia, liver cell granular or vacuolar degeneration and necrosis, karyolysis or pyknosis, glycogen granules decreasing, metanephros atrophy, metanephric canaliculus epithelium granular or vacuolar degeneration and necrosis, mitochondrion swelling and mitochondrion cristae disappearing, karyolysis, distal convolutal tubule microvilli desquamating, spleen marrow cell degeneration and necrosis, intercellular substance of spleen hyperplasia, spleen atresia, blood corpuscle disappearing.

后肾土黄色、淡褐色或苍白色,肾小管上皮细胞肿胀、颗粒或水泡变性、坏死,细胞内有大量血细胞流出,线粒体肿胀,嵴结构消失,细胞核溶解,肾间质甲状腺滤泡和拟淋巴细胞增生,远曲小管微绒毛脱落、管道细胞界限不清。心脏肌纤维肿胀、颗粒或空泡变性,严重的肌纤维溶解、变细或断裂,肌纤维间水肿、炎性细胞浸润,部分心肌细胞核浓缩。脾脏暗褐色,脾髓质细胞变性、坏死,拟淋巴细胞明显减少,淋巴细胞岛少见、岛中细胞成份减少,黑素巨噬细胞中心减少、体积缩小,脾脏网状基质水肿,脾窦闭锁,血细胞减少。

Following the puncture of zona pellucida with two holes by injection pipette that contained donor nuclei or cells, the injection pipette was pulled back to the perivitelline space while the negative pressure was increased in the holding pipette until the polar body and karyoplasm were wiped off completely. Then a reconstructed embryo was completed by the direct injection of the donor nucleus or cell without pulling out the injection pipette.

首先以预先吸有细胞核或细胞的注射针在固定于持卵针上的卵母细胞透明带上穿刺两个孔,然后一边缓慢地将注射针回拔至卵周隙中,一边逐渐增加持卵针中的负压,直至极体与目标核质被完整吸入持卵针中而完成去核,最后在不拔出注射针的情况下直接注射细胞核或完整细胞进而完成重构胚的构建。

Observ ed with transmission electron microscope and scanning electron microscope, t he apoptotic nuclei showed the typical morphological feathers of apoptosis: cond ensation and margination of chromatin, bubbling of nuclear membrane and apoptoti c bodies.

在透射电子显微镜和扫描电子显微镜下都观察到凋亡的细胞核呈现典型的凋亡形态学特征,例如染色质的凝集及趋边化,细胞核表面形成空泡化结构并有大量的凋亡小体结构出现。

It turned out that many inclusions were found in the nucleolus and the cytoplasm of PK15 cell. The paracrystalline arrays was found in the inclusion in cytoplasm whose electron dense is deep; The nuclear of PK15 cell dissolved and there were margination of multiple chromatin aggregates and suspected intranuclear viral inclusion in PK15 cell.

细胞质中包涵体电子密度深,位于核膜周围,内有呈晶格状排列的病毒粒子,直径12±2nm;细胞核内染色质消散,在细胞核内发现多个电子密度深的、圆形或环状、由大量异染色质组成的包涵体,内有细微的粒状物质。

Nuclear transfer technique is important not only for the study of nuclear-cytoplasminteraction,but also has extensive applications in human clinics and animal husbandry.

细胞核移植技术不仅是研究细胞核与细胞质相互作用的重要手段,而且是在医学和农业等领域都将有着广泛的应用前景的一项重要胚胎工程技术。

Nuclear transfer technique is important not only for the study of nuclear-cytoplasm interaction, but also has extensive applications in human clinics and animal husbandry. it has been proved that porcine somatic cloning is the most difficult among all mammalian species.

细胞核移植技术不仅是研究细胞核与细胞质相互作用的重要手段,而且是在医学和农业等领域都将有着广泛的应用前景的一项重要胚胎工程技术。

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